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Hyphenated Technique of LC-PDA -MS/MS for Phytochemical Profiling of Ficus deltoidea

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Crystallizing Ideas – The Role of Chemistry

Abstract

Ficus deltoidea is one of the widely used traditional herbal medicines in Malaysia. However, scientific data for this herb are very limited, particularly its phytochemical profile. The complexity of the phytochemical profile has hindered the development of this herb to wider application. Recent advancement of hybrid technology in analytical tools has eased the work of phytochemical profiling by simultaneous separation and identification using liquid chromatography integrated with photo diode array and tandem mass spectrometer (LC-PDA-MS/MS ). This hyphenated technique was successfully used in the present study. A wide range of phytochemicals including amino acids, organic acids, alkaloids, terpenoids, polyphenols, and their derivatives have been identified. The marker compound, isovitexin (apigenin) was identified at the retention time 17.5 min in the negative ion mode of mass spectrometry. Besides flavone (apigenin), this non-targeted mass screening detected isoflavones (genistein), flavanone (naringenin), flavonol (kaempferol), and flavanol (catechin) in the leaves extract of the plant. Several organic acids and phenolic acids which are very important for plant physiology and defense mechanism were also found in this study. The identification was carried out by matching the fragmentation pattern of the compound to the literature data. The presence of product ions which are the characteristic fragments for a particular compound was used for phytochemical identification. In other words, the fragmentation pattern of phytochemical is similar to the fingerprint of human beings.

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Acknowledgments

The author would like to acknowledge the financial support of Exploratory Research Grant Scheme (ERGS 4L114) from the Ministry of Education, Malaysia. I also appreciate the help of Dr. Manjunatha Devagondanahalli Hadagali who is a post-doctoral researcher in the laboratory preparing the plant crude extract for analysis.

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Authors and Affiliations

  1. Metabolites Profiling Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia, UTM Skudai, 81310, Johor Bahru, Johor, Malaysia

    Lee Suan Chua

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  1. Lee Suan Chua
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Correspondence to Lee Suan Chua .

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Editors and Affiliations

  1. Dep. of Chemistry, Fac. of Science, University of Mauritius, Reduit, Mauritius

    Ponnadurai Ramasami

  2. Dept of Chemistry, Faculty of Scien, University of Mauritius, Reduit, Mauritius

    Minu Gupta Bhowon

  3. Dept. of Chemistry, Faculty of Scie, University of Mauritius, Reduit, Mauritius

    Sabina Jhaumeer Laulloo

  4. Reduit, Mauritius

    Henri Li Kam Wah

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Chua, L.S. (2016). Hyphenated Technique of LC-PDA -MS/MS for Phytochemical Profiling of Ficus deltoidea . In: Ramasami, P., Gupta Bhowon, M., Jhaumeer Laulloo, S., Li Kam Wah, H. (eds) Crystallizing Ideas – The Role of Chemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-31759-5_4

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