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Deletion of an electron donor-binding subunit of the NDH-1 complex, NdhS, results in a heat-sensitive growth phenotype in Synechocystis sp. PCC 6803

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  • Marine & Freshwater Biology
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Chinese Science Bulletin

Abstract

In cyanobacteria and higher plants, NdhS is suggested to be an electron donor-binding subunit of NADPH dehydrogenase (NDH-1) complexes and its absence impairs NDH-1-dependent cyclic electron transport around photosystem I (NDH-CET). Despite significant advances in the study of NdhS during recent years, its functional role in resisting heat stress is poorly understood. Here, our results revealed that the absence of NdhS resulted in a serious heat-sensitive growth phenotype in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Furthermore, the rapid and significant increase in NDH-CET caused by heat treatment was completely abolished, and the repair of photosystem II under heat stress conditions was greatly impaired when compared to that of other photosynthetic apparatus in the thylakoid membrane. We therefore conclude that NdhS plays an important role in resistance to heat stress, possibly by stabilizing the electron input module of cyanobacterial NDH-1 complexes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370270), National Basic Research Program of China (2009CB118500), Shanghai Natural Science Foundation (14ZR1430000) and Project of Shanghai Education Committee (12ZZ132).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Life Sciences and Technology, Harbin Normal University, Harbin, 150025, China

    Jiaohong Zhao & Zijian Qiu

  2. College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, China

    Jiaohong Zhao, Fudan Gao, Quanxi Wang & Weimin Ma

Authors
  1. Jiaohong Zhao
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  2. Fudan Gao
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  3. Zijian Qiu
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  4. Quanxi Wang
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  5. Weimin Ma
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Corresponding authors

Correspondence to Quanxi Wang or Weimin Ma.

Additional information

Jiaohong Zhao and Fudan Gao contributed equally to this work.

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Zhao, J., Gao, F., Qiu, Z. et al. Deletion of an electron donor-binding subunit of the NDH-1 complex, NdhS, results in a heat-sensitive growth phenotype in Synechocystis sp. PCC 6803. Chin. Sci. Bull. 59, 4484–4490 (2014). https://doi.org/10.1007/s11434-014-0596-8

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