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Transcriptional responses of mu-, pi- and omega-class glutathione S-transferase genes in the hepatopancreas of Cipangopaludina cahayensis exposed to microcystin-LR

  • Article
  • Marine & Freshwater Biology
  • Published:
Chinese Science Bulletin

Abstract

The glutathione S-transferases (GSTs) play important roles in detoxification of microcystins (MCs), but the responses of GSTs to MC-LR have not been well characterized in freshwater gastropod, Cipangopaludina cahayensis. In the present study, we cloned full-length cDNAs of mu- and pi-class GSTs (GSTM and GSTP) and partial cDNA of omega-class GST (GSTO), and determined the transcriptional responses of the three GST genes to different concentrations of MC-LR (0, 1, 10 and 100 μg/L) in C. cahayensis. The full-length cDNAs of GSTM and GSTP of C. cahayensis were 813 and 820 bp, containing an open reading frame (ORF) of 648 bp (encoding 215 amino acids) and 624 bp (encoding 207 amino acids), respectively. The mRNA expression of GSTM and GSTO significantly decreased after exposure to 10 μg/L MC-LR, and the mRNA expression of GSTP significantly decreased after 100 μg/L MC-LR exposure. This might contribute to the detoxication of MCs in C. cahayensis, which is consistent with its sedentary life and filter-feeder status. The mRNA expression of the three GST isoforms in C. cahayensis could be used as biomarkers for water contamination.

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Acknowledgments

This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2012BAD25B04), the National Natural Science Foundation of China (31272641, 31172420), and the Fundamental Research Funds for the Central Universities (2013SC13).

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  1. College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, 430070, China

    Ling Li, Xu-Fang Liang, Shan He, Guangyu Li, Zhengyong Wen, Wenjing Cai & Dan Shen

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  1. Ling Li
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  2. Xu-Fang Liang
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Correspondence to Xu-Fang Liang.

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Li, L., Liang, XF., He, S. et al. Transcriptional responses of mu-, pi- and omega-class glutathione S-transferase genes in the hepatopancreas of Cipangopaludina cahayensis exposed to microcystin-LR. Chin. Sci. Bull. 59, 3153–3161 (2014). https://doi.org/10.1007/s11434-014-0305-7

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