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Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms

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Abstract

Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four Mg GSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and π were the most often observed interactions between BDE-47 and the GSTs.

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Authors and Affiliations

  1. Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai, 264003, China

    Fei Li  (李斐), Huifeng Wu  (吴惠丰), Qing Wang  (王清) & Jianmin Zhao  (赵建民)

  2. Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China

    Xuehua Li  (李雪花)

Authors
  1. Fei Li  (李斐)
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  2. Huifeng Wu  (吴惠丰)
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  3. Qing Wang  (王清)
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  4. Xuehua Li  (李雪花)
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  5. Jianmin Zhao  (赵建民)
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Correspondence to Huifeng Wu  (吴惠丰).

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Supported by the National Natural Science Foundation of China (No. 21107136) and the International Foundation for Science (No. F/5230-1)

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Li, F., Wu, H., Wang, Q. et al. Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms. Chin. J. Ocean. Limnol. 33, 705–713 (2015). https://doi.org/10.1007/s00343-015-4127-5

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