Abstract
Sponges (phylum Porifera) are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both ecological and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Reverse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water—E. coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was exposed to E. coli (1.1−107 CFU mL−1), V. anguillarum II (1.2−106 CFU mL−1) and V. alginolyticus (3.6−105 CFU mL−1) for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was observed on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The results demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
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Fu, W., Zhang, J., Zheng, C. et al. Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria. Chin. Sci. Bull. 58, 766–776 (2013). https://doi.org/10.1007/s11434-012-5400-z
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DOI: https://doi.org/10.1007/s11434-012-5400-z