Abstract
The mechanisms of oxygen evolution and carbon fixation in oxygenic organisms depend on the equal distribution of excitation energy to photosystems I and II, which is regulated by a mechanism referred to as light-state transition. In this work, a novel mechanism, energy spillover from PS I to PS II referred to as “inverse spillover”, was revealed besides “mobile phycobilisome (PBS)” and the “spillover” of energy from PS II to PS I in cyanobacteria. Under continuous illumination with blue light, time-dependent 77-K fluorescence spectra demonstrated heterogeneous kinetics for the PBS and photosystem components, indicating that inverse spillover and mobile PBS work successively to regulate the excitation to a balanced distribution in cyanobacterial cells under blue light. Inverse spillover and mobile PBS occur under both 100 and 300 μmol m−2 s−1 blue-light conditions but they are accelerated under the latter.
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References
Allen J F, Forsberg J. Molecular recognition in thylakoid structure and function. Trends Plant Sci, 2001, 6: 317–326
Bellafiore S, Barneche F, Peltier G, et al. State transitions and light adaptation require chloroplast thylakoid protein kinase STN7. Nature, 2005, 433: 892–895
Depege N, Bellafiore S, Rochaix J D. Role of chloroplast protein kinase Stt7 in LHCII phosphorylation and state transition in Chlamydomonas. Science, 2003, 299: 1572–1575
Allen J F. State transitions—A question of balance. Science, 2003, 299: 1530–1532
Allen J F, Holmes N G. A general model for regulation of photosynthetic unit function by protein phosphorlation. FEBS Lett, 1986, 202: 175–181
Murata N. Control of excitation transfer in photosynthesis. I. Light-induced change of chlorophyll a fluorescence in Porphyridium cruentum. Biochim Biophys Acta, 1969, 172: 242–251
Bruce D, Brimble S, Bryant D A. State transitions in a phycobili some-less mutant of the cyanobacterium Synechococcus sp. PCC 7002. Biochim Biophys Acta, 1989, 974: 66–73
Federman S, Malkin S, Scherz A. Excitation energy transfer in aggregates of Photosystem I and Photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: Can assembly of the pigment-protein complexes control the extent of spillover? Photosynth Res, 2000, 64: 199–207
Li Y, Zhang J, Xie J, et al. Temperature-induced decoupling of phycobilisomes from reaction centers. Biochim Biophys Acta, 2001, 1504: 229–234
Li D, Xie J, Zhao Y, et al. Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation. Biochim Biophys Acta, 2003, 1557: 35–40
Li D, Xie J, Zhao J, et al. Light-induced excitation energy redistribution in Spirulina platensis cells: “Spillover” or “mobile PBSs”? Biochim Biophys Acta, 2004, 1608: 114–121
Li H, Li D, Yang S, et al. The state transition mechanism-Simply depending on light-on and -off in Spirulina platensis. Biochim Biophys Acta, 2006, 1757: 1512–1519
Yang S, Zhang R, Hu C, et al. The dynamic behavior of phycobilisome movement during light state transitions in cyanobacterium Synechocystis PCC6803. Photosynth Res, 2009, 99: 99–106
Porra R J, Thompson W A. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents; verification of the concentration of chlorophyll standards by absorption spectroscopy. Biochim Biophys Acta, 1989, 975: 384–394
Zhao G, Han K. Site-specific solvation of the photoexcited protochlorophyllide a in methanol: Formation of the hydrogen-bonded intermediate state induced by hydrogen-bond strengthening. Biophysical J, 2008, 94: 38–46
Zhao G, Han K. Site-selective photoinduced electron transfer from alcoholic solvents to the chromophore facilitated by hydrogen bonding: A new fluorescence quenching mechanism. J Phys Chem B, 2007, 111: 8940–8945
Bald D. Supramolecular architecture of cyanobacterial thylakoid membranes: How is the phycobilisome connected with the photosystems? Photosynth Res, 1996, 49: 103–118
Kaňa R, Prášil O, Komárek O. GC papageorgiou, govindjee. Spectral characteristic of fluorescence induction in a model cyanobacterium Synechococcus sp. (PCC 7942). Biochim Biophys Acta, 2009, 1787: 1170–1178
Zhang R, Xie J, Hu C, et al. Hydrogen ions could directly regulate the oligomerization state of photosystem I in intact Spirulina platensis cells. Chin Sci Bull, 2008, 53: 317–320
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Xu, X., Zhang, R., Xie, J. et al. New mechanism revealed for light-state transition in cyanobacterium Arthrospira platensis according to 77-K fluorescence kinetics. Chin. Sci. Bull. 57, 1261–1265 (2012). https://doi.org/10.1007/s11434-011-4962-5
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DOI: https://doi.org/10.1007/s11434-011-4962-5