Abstract
Long-term preservation of human platelets will greatly reduce the risk of their shortage. Lyophilization has been proved feasible for this purpose. For the recovery of lyophilized platelets, rehydration is an important process. In this paper, the rehydration processes for 1 mL and 2 mL samples were studied. The effects of prehydration duration (15, 30, 60, 90, 120 and 150 min) in 37°C water vapor and the concentration of rehydration solution (25%, 50%, 75%, 100% platelet-poor plasma) on the recovery rate, MPV (mean platelet volume) and PDW (platelet distribution width) were investigated. The mass changes during the prehydration process were weighed. The optimized rehydration conditions are as follows: (1) for 1 mL sample, the prehydration duration was 15 min and for 2 mL sample the prehydration duration was 90 min; (2) the rehydration solution was 75% platelet-poor plasma. Under optimized conditions, the morphology of the rehydrated platelets kept normal and their ultrastructures kept intact, their aggregation capacity to thrombin (1 U/mL) was 82.8% of the fresh ones. These results will be helpful for designing the freeze-drying protocols for human platelets.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Kim S G, Chung C H, Kim Y K, et al. Use of particulate dentin- plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac Implants, 2002, 17: 86–94
Marx R E, Carlson E R, Eichstaedt R M, et al. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 1998, 85: 638–646
Bhanot S, Alex J C. Current applications of platelet gels in facial plastic surgery. Facial Plastic Surg, 2002, 18: 27–33
Bock M, Schleuning M, Heim M U, et al. Cryopreservation of human platelets with dimethyl-sulfoxide: Changes in biochemistry and cell function. Transfusion, 1995, 35: 921–924
Bakaltcheva I, O’sullivan A M, Hmel P, et al. Freeze-dried whole plasma: Evaluating sucrose, trehalose, sorbitol, mannitol and glycine as stabilizers. Thrombosis Res, 2007, 120: 105–116
Zhou X L, He H, Liu B L, et al. Effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized red blood cells. Cryo Lett, 2008, 29: 285–292
Shuja F, Shults C, Duggan M, et al. Development and testing of freeze-dried plasma for the treatment of trauma-associated coagulopathy. J Trauma, 2008, 65: 975–985
Fleming J A, Hoekstra E J, Moniaga V, et al. Reuse prevention syringes for reconstitution of lyophilized vaccines: Operational study and UNICEF plans for expanding introduction. Int J Occup Environ Health, 2009, 15: 9–13
Zhou J, Liu J H. Progress of research on lyophilized preservation of Platelets (in Chinese). J Exp Hematol, 2004, 12: 542–545
Read M S, Reddick R L, Bode A P, et al. Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: Potential for long-term storage of dried platelets for transfusion. Proc Natl Acad Sci USA, 1995, 92: 397–401
Wolkers W F, Walker N J, Tablin F, et al. Human platelets loaded with trehalose survive freeze-drying. Cryobiology, 2001, 42: 79–87
Wolkers W F, Walker N J, Tamari Y, et al. Towards a clinical application of freeze-dried human platelets. Cell Preserv Technol, 2003, 1: 175–188
Tang M K, Wolkers W F, Crowe J H, et al. Freeze-dried rehydrated human blood platelets regulate intracellular pH. Transfusion, 2006, 46: 1029–1037
Zhou X L, Zhu H, Zhang S Z, et al. Freeze-drying of human platelets: Influence of saccharide, freezing rate and cell concentration. Cryo Lett, 2007, 28: 187–96
Han Y, Quan G B, Liu X Z. Efect of reconstitution solutions on the recovery of lyophilized red blood cells (in Chinese). Chin J Blood Transfusion, 2004, 17: 10–12
Shu Z Q, Liu Z, Chen W W, et al. The rehydration injury study of freeze-dried red blood cells during rehydration. In: International Con-gress of Refrigeration, Beijing. 2007
Fan J L, Xu X G, Zhang S Z, et al. Experimental study on rehydration conditions of freeze-dried platelets. J Zhejiang Univ-SC A, 2009, 10: 697–703
Chen H. The assay sense of MPV, PCT and PDW of hemophiles’ platelets (in Chinese). Appl J General Practice, 2006, 4: 479
Wolkers W F, Tablin F, Crowe J H. From anhydrobiosis to freeze- drying of eukaryotic cells. Comp Biochem Physiol A, 2002, 131: 535–543
Acker J P, Fowler A, Lauman B, Crowe J H. Survival of desiccated mammalian cells: Beneficial effects of isotonic media. Cell Preserv Technol, 2002, 1: 129–140
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Rights and permissions
This article is published under an open access license. Please check the 'Copyright Information' section either on this page or in the PDF for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team.
About this article
Cite this article
Fan, J., Xu, X., Zhang, S. et al. Optimization study on the rehydration process of lyophilized human platelets. Chin. Sci. Bull. 56, 455–460 (2011). https://doi.org/10.1007/s11434-011-4381-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11434-011-4381-7