Abstract
The interaction of lumichrome (LC) as an endogenous fluorescence probe with nucleobases, nucleosides and nucleic acids has been studied by UV-visible absorption, fluorescence spectroscopy, polarized fluorescence and viscosity. The fluorescence of LC was strongly quenched by a series of nucleic acids and their precursors. The influences of the medium, temperature and salt effects on LC-nucleobase and LC-nucleic acid complexes were investigated. The influences of polarized fluorescence, thermal denaturation and viscosity on LC-ctDNA interaction were examined. The results demonstrate that the main binding model of LC-ctDNA includes strong intercalating into the DNA helix chain.
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Li, H., Jiang, Z. & Zhang, R. Fluorescence quenching and the binding interaction of lumichrome with nucleic acids. Chin. Sci. Bull. 55, 2829–2834 (2010). https://doi.org/10.1007/s11434-010-4008-4
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DOI: https://doi.org/10.1007/s11434-010-4008-4