Skip to main content
SpringerLink
Log in

The molecular mechanism of photodynamic therapy to fibrosis: Regulation on the pyridinoline cross-link formation in collagen

  • Articles/Chemical Biology
  • Published:
Chinese Science Bulletin

Abstract

Fibrotic processes in tissues are characterised by an excessive accumulation of collagen containing increased levels of pyridinoline cross-links, which has been taken as an important criterion to diagnose the fibrosis. Hypocrellin B (HB) is a natural photosensitizer with excellent photodynamic activity but very low dark-toxicity. In the current work, main purpose was to clarify if photodynamic action of HB could be applied to regulating the level of pyridinoline cross-link in collagen. To use gelatin as a model collagen, it was observed that the pyridinoline cross-link formation was effectively degraded by HB photodynamic action in the gelatin, suggesting the level of pyridinoline cross-link formation in collagen may be photodynamically regulated to a normal level. Furthermore, mechanism of the photodynamic action of HB was studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Webber J, Herman M, Kessel D, et al. Photodynamic treatment of neoplastic lesions of the gastrointestinal tract. Langenbeck Arch Surg, 2000, 385: 299–304

    Article  Google Scholar 

  2. Zhang J, Cao E H, Li J F, et al. Photodynamic effects of hypocrellin A on three human malignant cells by inducing apoptotic cell death. J Photochem Photobiol B: Biol, 1998, 43: 106–111

    Article  Google Scholar 

  3. Liu F G, Gu Y, Yu C Q, et al. Effect of photosensitizer photobleaching on vascular selectivity of photodynamic therapy (in Chinese). Chin J Laser Med Surg, 2002, 11: 137–140

    Google Scholar 

  4. Wang K, Gu Y, Liu F G, et al. Several question of Photodynamic Therapy treatment the Port wine stains in clinic (in Chinese). Chin J Laser Med Surg, 2002, 11: 190–192

    Google Scholar 

  5. Hulson J B, Zhou J, Chen J, et al. Hypocrellin, from Hypocrella bambuase, is phototoxic to human immunodeficiency virus. Photochem Photobiol, 1994, 60: 253–255

    Article  Google Scholar 

  6. Diwu Z J. Novel therapeutic and diagnostic applications of hypocrellins and hypericins. Photochem Photobiol, 1995, 61: 529–539

    Google Scholar 

  7. Kraus G A, Zhang W J, Febr M J, et al. Research at the interface between chemistry and virology: development of a molecular flashlight. Chem Rew, 1996, 96: 523–535

    Article  Google Scholar 

  8. Jiang L J. The structure of hypocrellins, character, photoreaction and the reaction mechanism (II). The reaction of hypocrellins. Chinese Sci Bull, 1990, 35: 1681–1690

    Google Scholar 

  9. Jiang L J, He Y Y. Photophysic, photochemistry and photobiology of hypocrelline. Chinese Sci Bull, 2000, 45: 2019–2032

    Google Scholar 

  10. Zhao B Z, Xie J, Zhao J Q. A novel water-soluble nanoparticles of hypocrellin B and their interaction with a model protein-C-phycocyanin. BBA-Gen Subjects, 2004, 1670: 113–120

    Article  Google Scholar 

  11. Huang N Y, Liu F G, Gu Y. Animal experiment of the port wine stains’ treatment by photodynamic therapy with two dosage form of hypocrellin B (in Chinese). Acta Laser Biol Sin, 2007, 16: 359–363

    Google Scholar 

  12. Dennis A H, David R E. Molecular site specificity of pyridinoline and pyrrole cross-links in type I collagen of human bone. J Biol Chem, 1996, 271: 26508–26516

    Article  Google Scholar 

  13. Trojanowska M, LeRoy E C, Krieg B E T. Pathogenesis of fibrosis: Type I collagen and the skin. J Mol Med, 1998, 76: 266–274

    Article  Google Scholar 

  14. Cleutjens J P, Verluyten M J, Smiths J F, et al. Collagen remodeling after myocardial infarction in the rat heart. Am J Pathol, 1995,147: 325–338

    Google Scholar 

  15. Milani S, Herbst H, Schuppan D, et al. In situ hybridization for procollagen type I, III and IV mRNA in normal and fibrotic rat liver; evidence for predominant expression in nonparenchymal liver cells. Hepatology, 1989, 10: 84–92

    Article  Google Scholar 

  16. Tsukamoto H, Matsuoka M, Blaner W, et al. Ito cell activation during progression of alcoholic liver fibrosis. In: Wisse E, Knook D L, McCuskey R S, eds. Cells of the Hepatic Sinusoid. Leiden: Kupffer Cell Foundation, 1991, 3: 453–456

    Google Scholar 

  17. van der Slot-Verhoevena A J, van Dura E A, Attema J, et al. The type of collagen cross-link determines the reversibility of experimental skin fibrosis. BBA-Mol Basis Dis, 2005, 1740: 60–67

    Google Scholar 

  18. Bailey A J, Bazin S, Sims T J, et al. Characterization of the collagen of human hypertrophic and normal scars. Biochim Biophys Acta, 1975, 405: 412–421

    Google Scholar 

  19. Last J A, King T E, Nerlich A G, et al. Collagen crosslinking in adult patients with acute and chronic fibrotic lung disease. Molecular markers for fibrotic collagen. Am Rev Respir Dis, 1990, 141: 307–313

    Google Scholar 

  20. van der Slot A J, Zuurmond A M, van den Bogaerdt A J, et al. Increased formation of pyridinoline cross-links due to higher telopeptide lysyl hydroxylase levels is a general fibrotic phenomenon. Matrix Biol, 2004, 23: 251–257

    Article  Google Scholar 

  21. Hayasaka A, Ilda S, Suzuki N, et al. Pyridinuline collagen cross-links in patients with chronic viral hepatitis and cirrhosis. J Hepatol, 1996,24: 692–698

    Article  Google Scholar 

  22. Robins S P, Stewart P, Astbury C, et al. Measurement of the cross linking compound, pyridinoline, in urine as an index of collagen degradation in joint disease. Ann Rheum Dis, 1986, 45: 969–973

    Article  Google Scholar 

  23. Ištok R, Bély M, Stančiková M, et al. Evidence for increased pyridinoline concentration in fibrotic tissues in diffuse systemic sclerosis. Clin Exp Dermatol, 2001, 26: 545–547

    Article  Google Scholar 

  24. Brinckmann J, Neess C M, Gaber Y, et al. Different pattern of collagen cross-links in two sclerotic skin diseases: Lipodermatosclerosis and circumscribed scleroderma. J Invest Dermatol, 2001, 117: 269–273

    Article  Google Scholar 

  25. Donnelly R F, McCarron P A, Cassidy C M, et al. Delivery of photosensitisers and light through mucus: Investigations into the potential use of photodynamic therapy for treatment of pseudomonas aeruginosa cystic fibrosis pulmonary infection. J Control Release, 2007,117: 217–226

    Article  Google Scholar 

  26. Statius van Eps R G, Mark L L, Schiereck J, et al. Photodynamic therapy inhibits the injury-induced fibrotic response of vascular smooth muscle cells. Eur J Vasc Endovasc Surg, 1999,18: 417–423

    Article  Google Scholar 

  27. Diestelhorst M, Grisanti S. Photodynamic therapy to control fibrosis in human glaucomatous eyes after trabeculectomy—A clinical pilot study. Archives of ophthalmology, 2002, 120: 130–134

    Google Scholar 

  28. Eastoe J E. The amino acid composition of mammalian collagen and gelatin. Biochem J, 1955, 61: 589–600

    Google Scholar 

  29. Cole C G B, Roberts J J. Gelatine fluorescence and its relationship to animal age and gelatine colour. SA J Food Sci Nutr, 1996, 8: 139–143

    Google Scholar 

  30. Uchiyama A, Ohishi T, Takahashi M, et al. Fluorophores from aging human articular cartilage. J Biochem, 1991, 110: 714–718

    Google Scholar 

  31. Moriguchi T, Fujimoto D. Crosslink of collagen in Hypertrophic Scar. J Invest Dermatol, 1979, 72: 143–145

    Article  Google Scholar 

  32. David R E, Ian R D, Kirk V. Collagen cross-linking in human bone and articular cartilage. Biochem J, 1988, 252: 495–500

    Google Scholar 

  33. Song L M. Study on the interaction of pigment/bio-molecules and recognition to the microenvironments of bio-molecules by the spectral responses of Hypocrellin B (in Chinese). Doctor Dissertation. Beijing: Institute of Beijing Chemistry, Chinese Academy of Sciences, 2006

    Google Scholar 

  34. Zhao B Z. Study on the interaction of pigment/protein and the clinical application of hypocrellin photodynamic drugs (in Chinese). Doctor Dissertation. Beijing: Institute of Beijing Chemistry, Chinese Academy of Sciences, 2005

    Google Scholar 

  35. Robins S P. Cross-linking of collagen Isolation, structural characterization and glycosylation of pyridinoline. Biochem J, 1983, 215: 167–173

    Google Scholar 

  36. Robins S P, Duncan A. Cross-linking of collagen Location of pyridinoline in bovine articular cartilage at two sites of the molecule. Biochem J, 1983, 215: 175–182

    Google Scholar 

  37. Song L M, Xie J, Zhang C X, et al. Recognition of various biomolecules by the environment-sensitive spectral responses of hypocrellin B. Photochem Photobiol Sci, 2007, 6: 683–688

    Article  Google Scholar 

  38. Song L M, Zhao B Z, Xie J, et al. Interactions of hypocrellin B with hyaluronan and photo-induced interactions. BBA-Gen Subjects, 2006, 1760: 333–339

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Natural Pharmaceutical and Chemical Biology of Yunnan Province, Honghe University, Mengzi, 661100, China

    JuCheng Zhang, Wei Liu, ZhongZhou Yi, Rui Chen, Ying Li & Yong Min

  2. School of Science, Honghe University, Mengzi, 661100, China

    JuCheng Zhang, Wei Liu, ZhongZhou Yi, Rui Chen, Ying Li & Yong Min

Authors
  1. JuCheng Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ZhongZhou Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rui Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ying Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yong Min
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei Liu.

Additional information

Supported by the Natural Science Foundation of Yunnan Province (Grant No. 2005C0078M) and Scientific Research Startup Project Foundation of Honghe University (Grant No. XSS07012)

About this article

Cite this article

Zhang, J., Liu, W., Yi, Z. et al. The molecular mechanism of photodynamic therapy to fibrosis: Regulation on the pyridinoline cross-link formation in collagen. Chin. Sci. Bull. 54, 2230–2234 (2009). https://doi.org/10.1007/s11434-009-0420-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-009-0420-z

Keywords

Search

Navigation