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The role of JDP2 in the differentiation of neuroblastoma SH-SY5Y cells

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  • Molecular Biology
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Chinese Science Bulletin

Abstract

c-Jun dimerization protein JDP2 is a member of the AP-1 family that serves as a repressor in gene transcription. To study the role of JDP2 in neuroblastoma cells, we have established a stably transfected SH-SY5Y cell line that carries jdp2 gene. Our results showed that JDP2 could promote neuronal differentiation of SH-SY5Y cells. The SY5Y-JDP2 cells exhibited lower percentage of cells in S phase and retarded growth rate than its parental cells that were accompanied by a higher expression of p21 and an intense reduction of the cyclin D1. In conclusion, the inhibitory role of JDP2 on neuroblastoma cell growth could be critical for the cells to be withdrawn from cell cycle and to proceed differentiation.

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Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Department of Molecular Biology & Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China

    Shen JinHua, Wu Meng, Wu NingHua, Zhang Ye & Shen YuFei

  2. Division of Hematology & Oncology, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, 44106, USA

    Shen JinHua

Authors
  1. Shen JinHua
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  2. Wu Meng
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  3. Wu NingHua
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  4. Zhang Ye
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  5. Shen YuFei
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Corresponding authors

Correspondence to Zhang Ye or Shen YuFei.

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Contributed equally to this work

Supported by the National Natural Science Foundation of China (Grant Nos. 90408007 and 30470376)

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Shen, J., Wu, M., Wu, N. et al. The role of JDP2 in the differentiation of neuroblastoma SH-SY5Y cells. Chin. Sci. Bull. 53, 227–232 (2008). https://doi.org/10.1007/s11434-008-0107-x

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  • DOI: https://doi.org/10.1007/s11434-008-0107-x

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