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Hierarchical recognition on the taxonomy of Nitzschia closterium f. minutissima

  • Articles
  • Marine Biology
  • Published:
Chinese Science Bulletin

Abstract

Nitzschia closterium f. minutissima, a marine eukaryotic unicellular diatom, originally classified as Bacillariophyta/Bacillariophyceae/Bacillariales/Bacillariaceae/Nitzschia, is one of the most important feed sources in mariculture. In this study, its morphological features were examined under DIC Microscopy (differential interference contrast microscope); its pigments and fatty acids composition were analyzed by using High Performance Liquid Chromatography (HPLC) and gas chromatography (GC); the complete Actin cDNA, part 18S rDNA, complete ITS1 and ITS2 sequences, part 28S rDNA sequences, and a putatively encoding Δ5 fatty acid desaturase gene were cloned respectively and further functioned in transgenic yeast. The sequence alignments were separately conducted using the related sequences from Nitzschia closterium f. minutissima, Cylindrotheca closterium (Bacillariophyta/Bacillariales/Bacillariaceae/Cylindrotheca) and Phaeodactylum tricornutum (Naviculales) with ClustalX 1.83. No distinct difference was discovered between N. closterium f. minutissima and P. tricornutum in both biochemical and molecular level. Their identity was more than 99.6% among 18S rDNA, 5.8S rDNA and actin-gene sequences, and is up to 98.6% even among ITS1 and ITS2 sequences. Their Δ5 desaturase similarity was 99.4%. However, the lower similarity was disclosured between N. closterium f. minutissima and Cylindrotheca closterium, which shared less than 40% identity in the ITS1 and ITS2 sequences. So, N. closterium f. minutissima should not be placed in Bacillariales, Bacillariaceae, Nitzschia, but in Naviculales, Phaeodactylaceae, Phaeodactylum, and it was actually a strain of P. tricornutum.

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Authors and Affiliations

  1. Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Shi Juan, Pan KeHou & Zhu BaoHua

  2. Key Laboratory of Bioresources and Eco-environment of Ministry of Education, School of Life Sciences, Sichuan University, Chengdu, 610064, China

    Wang XiaoQing, Chen Fang, Zhou Mi & Qing RenWei

Authors
  1. Shi Juan
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  2. Pan KeHou
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  3. Wang XiaoQing
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  4. Chen Fang
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  5. Zhou Mi
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  6. Zhu BaoHua
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  7. Qing RenWei
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Corresponding authors

Correspondence to Pan KeHou or Qing RenWei.

Additional information

Supported by the National Basic Research Program of China (Grant No. 2005CCA02400)

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Shi, J., Pan, K., Wang, X. et al. Hierarchical recognition on the taxonomy of Nitzschia closterium f. minutissima. Chin. Sci. Bull. 53, 245–250 (2008). https://doi.org/10.1007/s11434-008-0080-4

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  • DOI: https://doi.org/10.1007/s11434-008-0080-4

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