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RadA: A protein involved in DNA damage repair processes of Deinococcus radiodurans R1

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Chinese Science Bulletin

Abstract

RadA is highly conserved in bacteria and belongs to the RecA/RadA/Rad51 protein superfamily found in bacteria, archaea and eukarya. In Archaea, it plays a critical role in homologous recombination process due to its RecA-like function. In Escherichia coli, it takes part in conjugational recombination and DNA repair but is not as important as that of archaea. Using PSI-BLAST searches, we found that Deinococcus radiodurans RadA had a higher similarity to that of bacteria than archaea and eukarya. Disruption of radA gene in D. radiodurans resulted in a modestly decreased resistance to gamma radiation and ultraviolet, but had no effect on the resistance to hydrogen peroxide. Complementation of the radA disruptant by both E. coli radA and D. radiodurans radA could fully restore its resistance to gamma radiation and ultraviolet irradiation. Further domain function analyses of D. radiodurans RadA showed that the absence of the zinc finger domain resulted in a slightly more sensitive phenotype to gamma and UV radiation than that of the radA mutant, while the absence of the Lon protease domain exhibited a slightly increased resistance to gamma and UV radiation. These data suggest that D. radiodurans RadA does play an important role in the DNA damage repair processes and its three different domains have different functions.

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Authors and Affiliations

  1. Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China

    Zhou Qing, Zhang Xinjue, Xu Hong, Xu Bujin & Hua Yuejin

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  1. Zhou Qing
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  2. Zhang Xinjue
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  3. Xu Hong
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  4. Xu Bujin
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  5. Hua Yuejin
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Correspondence to Hua Yuejin.

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Zhou, Q., Zhang, X., Xu, H. et al. RadA: A protein involved in DNA damage repair processes of Deinococcus radiodurans R1. CHINESE SCI BULL 51, 2993–2999 (2006). https://doi.org/10.1007/s11434-006-2209-7

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  • DOI: https://doi.org/10.1007/s11434-006-2209-7

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