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Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis

Science China Life Sciences volume 59, pages 622–626 (2016)Cite this article

Abstract

In the Dongting water system, the Carassius auratus (Crucian carp) complex is characterized by the coexistence of diploid forms (2n=100, 2nCC) and polyploid forms. Chromosomal and karyotypic analyses have suggested that the polyploid C. auratus has a triploid (3n=150, 3nCC) and a tetraploid origin (4n=200), respectively. However, there is a lack of direct genetic evidence to support this conclusion. In this paper, analysis of the 5S rDNA chromosomal locus revealed that the 3nCC is of triploid origin. Analysis of the species-specific chromosomal centromere locus revealed that 3nCC individuals possess three sets of C. auratus-derived chromosomes. Our results provide direct cytogenetic evidence suggesting that individuals with 150 chromosomes are of autotriploid origin within the C. auratus complex. It marks an important contribution to the study of polyploidization and the evolution of vertebrates.

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Affiliations

  1. Key Laboratory of Protein Chemistry and Fish Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Qinbo Qin, Juan Wang, Min Hu, Shengnan Huang & Shaojun Liu

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  1. Qinbo Qin
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  2. Juan Wang
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  3. Min Hu
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  4. Shengnan Huang
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  5. Shaojun Liu
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Correspondence to Shaojun Liu.

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Qin, Q., Wang, J., Hu, M. et al. Autotriploid origin of Carassius auratus as revealed by chromosomal locus analysis. Sci. China Life Sci. 59, 622–626 (2016). https://doi.org/10.1007/s11427-016-5040-7

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  • DOI: https://doi.org/10.1007/s11427-016-5040-7

Keywords

  • autotriploids
  • chromosomal loci
  • polyploidization
  • evolution