Abstract
Oxidative stress, regarded as a negative effect of free radicals in vivo, takes place when organisms suffer from harmful stimuli. Some viruses can induce the release of reactive oxygen species (ROS) in infected cells, which may be closely related with their pathogenicity. In this report, chaetocin, a fungal metabolite reported to have antimicrobial and cytostatic activity, was studied for its effect on the activation of latent Epstein-Barr virus (EBV) in B95-8 cells. We found that chaetocin remarkably up-regulated EBV lytic transcription and DNA replication at a low concentration (50 nmol L−1). The activation of latent EBV was accompanied by an increased cellular ROS level. N-acetyl-L-cysteine (NAC), an ROS inhibitor, suppressed chaetocin-induced EBV activation. Chaetocin had little effect on histone H3K9 methylation, while NAC also significantly reduced H3K9 methylation. These results suggested that chaetocin reactivates latent EBV primarily via ROS pathways.
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Acknowledgements
Shilun Zhang, Juan Yin and Jiang Zhong conceived and designed the study and manuscript writing. Shilun Zhang and Jiang Zhong contributed to the data processing and manuscript editing. All authors contributed to the discussion and provided comments on the manuscript.
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Zhang, S., Yin, J. & Zhong, J. Chaetocin reactivates the lytic replication of Epstein-Barr virus from latency via reactive oxygen species. Sci. China Life Sci. 60, 66–71 (2017). https://doi.org/10.1007/s11427-016-0286-7
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DOI: https://doi.org/10.1007/s11427-016-0286-7