Abstract
Cyclin B is an important regulator of the cell cycle G2 to M phase transition. The silkworm genomic database shows that there are two Cyclin B genes in the silkworm (Bombyx mori), BmCyclin B and BmCyclin B3. Using silkworm EST data, the cyclin B3 (EU074796) gene was cloned. Its complete cDNA was 1665 bp with an ORF of 1536 bp derived from seven exons and six introns. The BmCyclin B3 gene encodes 511 amino acids, and the predicted molecular weight is 57.8 kD with an isoelectric point of 9.18. The protein contains one protein damage box and two cyclin boxes. RNA interference-mediated reduction of BmCyclin B and BmCyclin B3 expression induced cell cycle arrest in G2 or M phase in BmN-SWU1 cells, thus inhibiting cell proliferation. These results suggest that BmCyclin B and BmCyclin B3 are necessary for completing the cell cycle in silkworm cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Minshull J, Blow J, Hunt T. Translation of cyclin mRNA is necessary for extracts of activated Xenopus eggs to enter mitosis. Cell, 1989, 56: 947–956
Murray A, Kirschner M. Cyclin synthesis drives the early embryonic cell cycle. Nature, 1989, 339: 275–286
Westendorf J, Swenson K, Ruderman J. The role of cyclin B in meiosis I. J Cell Biol, 1989, 108: 1431–1444
Glotzer M, Murray A W, Kirschner M W. Cyclin is degraded by the ubiquitin pathway. Nature, 1991, 349: 132–138
King R W, Deshaies R J, Peters J M, et al. How proteolysis drives the cell cycle. Science, 1996, 274: 1652–1658
King R W, Glotzer M, Kirschner M W. Mutagenic analysis of the destruction signal of mitotic cyclins and structural characterization of ubiquitinated intermediates. Mol Biol Cell, 1996, 7: 1343–1357
Jacobs H W, Knoblich J A, Lehner C F. Drosophila Cyclin B3 is required for female fertility and is dispensable for mitosis like Cyclin B. Genes Dev, 1998, 12: 3741–3751
Pines J, Hunter T. Human cyclin A and cyclin B1 are differentially located in the cell and undergo cell cycle dependent nuclear transport. J Cell Biol, 1991, 115: 1–17
Jackman M, Firth M, Pines J. Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus. EMBO J, 1995, 14: 1646–1654
Gallant P, Nigg E A. Identification of a novel vertebrate cyclin: Cyclin B3 shares properties with both A-type and B-type cyclins. EMBO J, 1994, 13: 595–605
Kreutzer M A, Richards J P, De Silva-Udawatta M N, et al. Caenorhabditis elegans cyclin A-type and B-type genes: A cyclin A multigene family, an ancestral cyclin B3 and differential germline expression. J Cell Sci, 1995, 108: 2415–2424
Sigrist S, Jacobs H, Stratmann R, et al. Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3. EMBO J, 1995, 14: 4827–4838
Takahashi M, Niimi T, Ichimura H, et al. Cloning of a B-type cyclin homolog from Bombyx mori and the profiles of its mRNA level in non-diapause and diapause eggs. Dev Genes Evol, 1996, 206: 288–291
Baluchamy S, Gopinathan K P. Characterization of a cyclin homolog from Bombyx mori nucleopolyhedrovirus. Virus Res, 2005, 108: 69–81
Pan M H, Cai X J, Liu M, et al. Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori. Tissue Cell, 2010, 42: 42–46
Tamura K, Dudley J, Nei M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24: 1596–1599
Dhawan S, Gopinathan K P. Cell cycle events during the development of the silk glands in the mulberry silkworm Bombyx mori. Dev Genes Evol, 2003, 213: 435–444
Zhang C D, Li F F, Chen X Y, et al. DNA replication events during larval silk gland development in the silkworm, Bombyx mori. J Insect Physiol, 2012, 58: 974–978
Perdrix-Gillot S. DNA synthesis and endomitoses in the giant nuclei of the silkgland of Bombyx mori. Biochimie, 1979, 61: 171–204
Zimmet J, Ravid K. Polyploidy: occurrence in nature, mechanisms, and significance for the megakaryocyte-platelet system. Exp Hematol, 2000, 28: 3–16
Gage L P. Polyploidization of the silk gland of Bombyx mori. J Mol Biol, 1974, 86: 97–108
Edgar B A, Orr-Weaver T L. Endoreplication cell cycles: more for less. Cell, 2001, 105: 297–306
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Pan, M., Hong, K., Chen, X. et al. BmCyclin B and BmCyclin B3 are required for cell cycle progression in the silkworm, Bombyx mori. Sci. China Life Sci. 56, 360–365 (2013). https://doi.org/10.1007/s11427-013-4459-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-013-4459-3