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Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

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Abstract

To understand the development of rice leaf blades, we identified a new rolled-leaf mutant, w32, from indica cultivar IR64 through EMS mutagenesis. The mutant showed a stable rolled-leaf phenotype throughout the life cycle. Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64. Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene. To determine the location of the gene, bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7. The rolled-leaf gene, tentatively named rl11(t), is likely a new gene as no other rolled-leaf genes have been identified near the region. By developing new SSR and InDel markers, the gene was delimited to a 52 kb region near the end of the short chromosome arm. Further fine mapping and cloning of the gene are currently underway.

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Authors and Affiliations

  1. Chinese National Center for Rice Improvement/National key Laboratory for Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    YongFeng Shi, Jie Chen, WenQiang Liu, QiNa Huang & JianLi Wu

  2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310016, China

    YongFeng Shi, QiNa Huang & Bo Shen

  3. International Rice Research Institute, Metro Manila DAPO Box 7777, Manila, Philippines

    Hei Leung

Authors
  1. YongFeng Shi
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  2. Jie Chen
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  3. WenQiang Liu
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  4. QiNa Huang
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  5. Bo Shen
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  6. Hei Leung
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  7. JianLi Wu
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Correspondence to JianLi Wu.

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Supported by the National High Technology Research and Development Program of China (Grant Nos. 2006AA10Z1E8 and 2006AA100101)

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Shi, Y., Chen, J., Liu, W. et al. Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.). SCI CHINA SER C 52, 885–890 (2009). https://doi.org/10.1007/s11427-009-0109-1

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  • DOI: https://doi.org/10.1007/s11427-009-0109-1

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