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Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast

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Abstract

Influenza A virus NS1 protein is an important regulatory factor with multiple functions and contributes greatly to viral pathogenesis. In the present study, transcription-activating potential of NS1 from different influenza A virus subtypes was examined in yeast two-hybrid system. The bait vectors containing different NS1 genes, along with an empty prey vector, were transformed into yeast AH109(for growth assay on QDO plate and α-galactosidase assay), and Y187(for β-galactosidase assay). AH109 transformants with NS1 gene from H1N1, H5N1, and H9N2 viruses grew vigorously on the QDO plate and secreted high level of α-galactosidase. Also, Y187 bearing the above NS1 genes exhibited enhanced β-galactosidase activity. Nevertheless, H3N2-NS1-transformed AH109 and Y187 yeasts did not grow on QDO plate and secrete β-galactosidase, respectively. These findings denote the remarkable variation in NS1 proteins from different influenza A virus subtypes on the transcription-stimulating capability in yeast.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Shantou University Medical College, Shantou, 515041, China

    WeiZhong Li, GeFei Wang, Jun Zeng, DanGui Zhang, Heng Zhang, XiaoXuan Chen, YouYing Chen & KangSheng Li

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  1. WeiZhong Li
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  2. GeFei Wang
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  3. Jun Zeng
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  4. DanGui Zhang
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  5. Heng Zhang
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  6. XiaoXuan Chen
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  7. YouYing Chen
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  8. KangSheng Li
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Correspondence to KangSheng Li.

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Supported by National Natural Science Foundation of China(Grant No. 30771988), and Funds for Outstanding Young Scientists in Universities of Guangdong Province (Grant No. LYM08056)

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Li, W., Wang, G., Zeng, J. et al. Differential transcription-activating capability of NS1 proteins from different influenza virus subtypes expressed in yeast. SCI CHINA SER C 52, 545–550 (2009). https://doi.org/10.1007/s11427-009-0072-x

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  • DOI: https://doi.org/10.1007/s11427-009-0072-x

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