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Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of Pseudomonas aeruginosa

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Abstract

The aiiA gene from Bacillus thuringiensis was cloned into the Pseudomonas/E. coli shuttle vector and transformed into Pseudomonas aeruginosa strain PAO1. Western blotting showed that the AiiA protein was expressed in PAO1. After induction by IPTG for 6 h and 18 h, expression of the aiiA gene in PAO1 completely degraded the quorum sensing autoinducers N-acylhomoserine lactones (AHLs): N-oxododecanoyl-L-homoserine lactone (OdDHL) and N-butyryl-L-homoserine lactone (BHL). The reduced amount of AHLs in PAO1 was also correlated with decreased expression and production of several virulence factors such as elastase and pyocyanin. AiiA expression also influenced bacterial swarming motility. Most importantly, our studies indicated that aiiA played significant roles in P. aeruginosa biofilm formation and dispersion, as observed by the differences of the biofilm formation on liquid and solid surfaces, and biofilm structures under a scanning electron microscope.

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Authors and Affiliations

  1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Wang Yao, Dai Yue, Zhang Yong, Hu YangBo, Yang BaoYu & Chen ShiYun

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Dai Yue, Zhang Yong & Hu YangBo

Authors
  1. Wang Yao
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  2. Dai Yue
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  3. Zhang Yong
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  4. Hu YangBo
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  5. Yang BaoYu
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  6. Chen ShiYun
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Correspondence to Chen ShiYun.

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These authors contributed equally to this work

Supported by the National Natural Science Foundation of China (Grant No. 30570020) and Natural Science Foundation of Hubei Province of China (Grant No. 2004ABA120)

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Wang, Y., Dai, Y., Zhang, Y. et al. Effects of quorum sensing autoinducer degradation gene on virulence and biofilm formation of Pseudomonas aeruginosa . SCI CHINA SER C 50, 385–391 (2007). https://doi.org/10.1007/s11427-007-0044-y

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  • DOI: https://doi.org/10.1007/s11427-007-0044-y

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