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The application of CRISPR-Cas in disease diagnosis and treatment

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Abstract

Clustered regularly interspaced short palindromic repeat (CRISPR) has been gaining much attention in the modern medical field and has been widely used for the diagnosis and treatment of diseases in recent years. In this review, we will introduce the application of CRISPR in disease diagnosis and treatment, including its use in detecting pathogens, gene mutations, and genetic diseases, as well as its application in gene therapy for single-gene diseases, cancer, viral infectious diseases, and cardiovascular diseases. Additionally, we will discuss the potential future directions and challenges of CRISPR in the diagnosis and treatment of diseases, and provide a thorough overview of the ways in which CRISPR is used for diagnosing and treating diseases.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21907077, 92153303, 21721005, 91940000) and Fundamental Research Funds for the Central Universities (2042023kf0118).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Wuhan University, Wuhan, 430071, China

    Yafen Wang

  2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China

    Zhiying Wang, Yin Liu & Xiang Zhou

  3. Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, 430071, China

    Xiang Zhou

  4. Cross Research Institute of Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    Xiang Zhou

  5. Department of Hematology of Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    Yin Liu & Fuling Zhou

  6. Department of Chemistry, College of Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Zhiying Wang

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Correspondence to Fuling Zhou or Yafen Wang.

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Wang, Z., Liu, Y., Zhou, F. et al. The application of CRISPR-Cas in disease diagnosis and treatment. Sci. China Chem. 66, 2734–2742 (2023). https://doi.org/10.1007/s11426-023-1765-0

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