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Multifunctional aggregates for precise cellular analysis

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Abstract

Precisely analyzing the target materials in living cells can reveal the essence and mystery of life at a deeper level, which will provide reliable theoretical basis for the occurrence, development, treatment and prognosis of major diseases. However, because living cells are in the dynamic process of metabolism, there are several challenges existed in accurate analysis, including subcellular compartment heterogeneity, plasma membrane interface barrier, and cell cycle regulation. In this regard, our group has designed and synthesized a series of multifunctional aggregates by mainly integrating the peptide elements, nucleic acid elements and aggregation elements to overcome the barriers. This article summarizes the latest developments of multifunctional aggregates for precise cell analysis by our group, and systematically introduces them according to different design concepts and targeting dimensions, such as space, efficiency as well as time. We hope this work could contribute to analyzing the biomarkers in cells through constructing the multifunctional aggregates, understanding the operation mechanism of cells, finally inspiring technology breakthroughs in biomedical fields.

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References

  1. Etzioni R, Urban N, Ramsey S, McIntosh M, Schwartz S, Reid B, Radich J, Anderson G, Hartwell L. Nat Rev Cancer, 2003, 3: 243–252

    Article  CAS  PubMed  Google Scholar 

  2. Carlton JG, Jones H, Eggert US. Nat Rev Mol Cell Biol, 2020, 21: 151–166

    Article  CAS  PubMed  Google Scholar 

  3. Mayor S, Pagano RE. Nat Rev Mol Cell Biol, 2007, 8: 603–612

    Article  CAS  PubMed  Google Scholar 

  4. Artus J, Cohen-Tannoudji M. Mol Cellular Endocrinol, 2008, 282: 78–86

    Article  CAS  Google Scholar 

  5. Malumbres M, Barbacid M. Nat Rev Cancer, 2009, 9: 153–166

    Article  CAS  PubMed  Google Scholar 

  6. Zhang P, Cui Y, Anderson CF, Zhang C, Li Y, Wang R, Cui H. Chem Soc Rev, 2018, 47: 3490–3529

    Article  CAS  PubMed  Google Scholar 

  7. Wang W, Hu Z. Adv Mater, 2019, 31: 1804827

    Article  CAS  Google Scholar 

  8. Choi J, Majima T. Chem Soc Rev, 2011, 40: 5893–5909

    Article  CAS  PubMed  Google Scholar 

  9. Sun H, Ren J, Qu X. Acc Chem Res, 2016, 49: 461–470

    Article  CAS  PubMed  Google Scholar 

  10. Meng HM, Liu H, Kuai H, Peng R, Mo L, Zhang XB. Chem Soc Rev, 2016, 45: 2583–2602

    Article  CAS  PubMed  Google Scholar 

  11. Watson JD, Crick FHC. Nature, 1953, 171: 737–738

    Article  CAS  PubMed  Google Scholar 

  12. Zhao W, Ali MM, Brook MA, Li Y. Angew Chem Int Ed, 2008, 47: 6330–6337

    Article  CAS  Google Scholar 

  13. Huang Y, Liu X, Shi M, Zhao S, Hu K, Chen ZF, Liang H. Chem Asian J, 2014, 9: 2755–2760

    Article  CAS  PubMed  Google Scholar 

  14. Luo J, Xie Z, Lam JWY, Cheng L, Tang BZ, Chen H, Qiu C, Kwok HS, Zhan X, Liu Y, Zhu D. Chem Commun, 2001, 1740–1741

  15. Jiang R, Dai J, Dong X, Wang Q, Meng Z, Guo J, Yu Y, Wang S, Xia F, Zhao Z, Lou X, Tang BZ. Adv Mater, 2021, 33: 2101158

    Article  CAS  Google Scholar 

  16. Qian J, Tang BZ. Chem, 2017, 3: 56–91

    Article  CAS  Google Scholar 

  17. Xia F, Wu J, Wu X, Hu Q, Dai J, Lou X. Acc Chem Res, 2019, 52: 3064–3074

    Article  CAS  PubMed  Google Scholar 

  18. Hu J-, Jiang W, Yuan L, Duan C, Yuan Q, Long Z, Lou X, Xia F. Aggregate, 2021, 2: 48–65

    Article  Google Scholar 

  19. Dai J, Wu X, Ding S, Lou X, Xia F, Wang S, Hong Y. J Med Chem, 2020, 63: 1996–2012

    Article  CAS  PubMed  Google Scholar 

  20. Cheng Y, Sun C, Ou X, Liu B, Lou X, Xia F. Chem Sci, 2017, 8: 4571–4578

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Grivennikov SI, Greten FR, Karin M. Cell, 2010, 140: 883–899

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Mantovani A, Allavena P, Sica A, Balkwill F. Nature, 2008, 454: 436–444

    Article  CAS  PubMed  Google Scholar 

  23. Guo H, Callaway JB, Ting JPY. Nat Med, 2015, 21: 677–687

    Article  PubMed  PubMed Central  Google Scholar 

  24. Cheng Y, Dai J, Sun C, Liu R, Zhai T, Lou X, Xia F. Angew Chem Int Ed, 2018, 57: 3123–3127

    Article  CAS  Google Scholar 

  25. Dowdy SF. Nat Biotechnol, 2017, 35: 222–229

    Article  CAS  PubMed  Google Scholar 

  26. Whitehead KA, Langer R, Anderson DG. Nat Rev Drug Discov, 2009, 8: 129–138

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Cheng Y, Sun C, Liu R, Yang J, Dai J, Zhai T, Lou X, Xia F. Angew Chem Int Ed, 2019, 58: 5049–5053

    Article  CAS  Google Scholar 

  28. Wei Y, Tang T, Pang HB. Nat Commun, 2019, 10: 3646

    Article  PubMed  PubMed Central  Google Scholar 

  29. Pang HB, Braun GB, Ruoslahti E. Sci Adv, 2015, 1: e1500821

    Article  PubMed  PubMed Central  Google Scholar 

  30. Islam MA, Xu Y, Tao W, Ubellacker JM, Lim M, Aum D, Lee GY, Zhou K, Zope H, Yu M, Cao W, Oswald JT, Dinarvand M, Mahmoudi M, Langer R, Kantoff PW, Farokhzad OC, Zetter BR, Shi J. Nat Biomed Eng, 2018, 2: 850–864

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Fan W, Yung B, Huang P, Chen X. Chem Rev, 2017, 117: 13566–13638

    Article  CAS  PubMed  Google Scholar 

  32. Wang Y, Kohane DS. Nat Rev Mater, 2017, 2: 17020

    Article  CAS  Google Scholar 

  33. Stewart MP, Sharei A, Ding X, Sahay G, Langer R, Jensen KF. Nature, 2016, 538: 183–192

    Article  CAS  PubMed  Google Scholar 

  34. Panariti A, Miserocchi G, Rivolta I. Nanotechnol Sci Appl, 2012, 5: 87–100

    CAS  PubMed  PubMed Central  Google Scholar 

  35. Yang J, Dai J, Wang Q, Cheng Y, Guo J, Zhao Z, Hong Y, Lou X, Xia F. Angew Chem Int Ed, 2020, 59: 20405–20410

    Article  CAS  Google Scholar 

  36. Cheng Y, Huang F, Min X, Gao P, Zhang T, Li X, Liu B, Hong Y, Lou X, Xia F. Anal Chem, 2016, 88: 8913–8919

    Article  CAS  PubMed  Google Scholar 

  37. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. CA-A Cancer J Clin, 2018, 68: 394–424

    Article  Google Scholar 

  38. Lheureux S, Braunstein M, Oza AM. CA-A Cancer J Clin, 2019, caac.21559

  39. Dai J, Cheng Y, Wu J, Wang Q, Wang W, Yang J, Zhao Z, Lou X, Xia F, Wang S, Tang BZ. ACS Nano, 2020, 14: 14698–14714

    Article  PubMed  Google Scholar 

  40. Yi X, Hu JJ, Dai J, Lou X, Zhao Z, Xia F, Tang BZ. ACS Nano, 2021, 15: 3026–3037

    Article  CAS  PubMed  Google Scholar 

  41. Zhen S, Yi X, Zhao Z, Lou X, Xia F, Tang BZ. Biomaterials, 2019, 218: 119330

    Article  CAS  PubMed  Google Scholar 

  42. Yi X, Dai J, Han Y, Xu M, Zhang X, Zhen S, Zhao Z, Lou X, Xia F. Commun Biol, 2018, 1: 202

    Article  PubMed  PubMed Central  Google Scholar 

  43. Coffman JA. Dev Cell, 2004, 6: 321–327

    Article  CAS  PubMed  Google Scholar 

  44. Nishitani H, Lygerou Z. Genes Cells, 2002, 7: 523–534

    Article  CAS  PubMed  Google Scholar 

  45. Kar S. Cell Syst, 2016, 2: 8–10

    Article  CAS  PubMed  Google Scholar 

  46. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PLC, Coviello GM, Wright WE, Weinrich SL, Shay JW. Science, 1994, 266: 2011–2015

    Article  CAS  PubMed  Google Scholar 

  47. Lou X, Zhuang Y, Zuo X, Jia Y, Hong Y, Min X, Zhang Z, Xu X, Liu N, Xia F, Tang BZ. Anal Chem, 2015, 87: 6822–6827

    Article  CAS  PubMed  Google Scholar 

  48. Zhuang Y, Zhang M, Chen B, Duan R, Min X, Zhang Z, Zheng F, Liang H, Zhao Z, Lou X, Xia F. Anal Chem, 2015, 87: 9487–9493

    Article  CAS  PubMed  Google Scholar 

  49. Zhuang Y, Xu Q, Huang F, Gao P, Zhao Z, Lou X, Xia F. ACS Sens, 2016, 1: 572–578

    Article  CAS  Google Scholar 

  50. Zhuang Y, Huang F, Xu Q, Zhang M, Lou X, Xia F. Anal Chem, 2016, 88: 3289–3294

    Article  CAS  PubMed  Google Scholar 

  51. Zhuang Y, Shang C, Lou X, Xia F. Anal Chem, 2017, 89: 2073–2079

    Article  CAS  PubMed  Google Scholar 

  52. Wu X, Wu J, Dai J, Chen B, Chen Z, Wang S, Wu F, Lou X, Xia F. Natl Sci Rev, 2021, doi: https://doi.org/10.1093/nsr/nwaa306

  53. Li J, Gao H, Liu R, Chen C, Zeng S, Liu Q, Ding D. Sci China Chem, 2020, 63: 1428–1434

    Article  CAS  Google Scholar 

  54. Ou H, Dai S, Liu R, Ding D. Sci China Chem, 2019, 62: 929–932

    Article  CAS  Google Scholar 

  55. Ni X, Zhang X, Duan X, Zheng HL, Xue XS, Ding D. Nano Lett, 2019, 19: 318–330

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key R&D Program of China (2020YFA0211200), the National Natural Science Foundation of China (22090050, 21974128, 21874121, 52003257), and Hubei Provincial Natural Science Foundation of China (2019CFA043, 2020CFA037).

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Authors and Affiliations

  1. State Key Laboratory of Biogeology and Environmental Geology, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, China

    Jing-Jing Hu, Xiaoqi Dong, Wenlian Jiang, Fan Xia & Xiaoding Lou

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  1. Jing-Jing Hu
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  2. Xiaoqi Dong
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  3. Wenlian Jiang
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  4. Fan Xia
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  5. Xiaoding Lou
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Correspondence to Xiaoding Lou.

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Conflict of interest The authors declare no conflict of interest.

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Hu, JJ., Dong, X., Jiang, W. et al. Multifunctional aggregates for precise cellular analysis. Sci. China Chem. 64, 1938–1945 (2021). https://doi.org/10.1007/s11426-021-1051-9

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