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In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay

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Abstract

The discovery and application of analyte-triggered fluorophore generation or fluorogenic reaction are significant and beneficial to the development of novel fluorescence (FL) analysis method. In this study, for the first time, we have reported a fluorogenic reaction to prepare fluorescent silicon-containing polymer dots (Si-PDs) by simply mixing N-[3-(trimethoxysilyl)propyl]ethylenediamine (DAMO) and hydroquinone (HQ) in aqueous solution at ambient temperature. Inspired by the alkaline phosphatase (ALP)-catalyzed hydrolysis of the substrate sodium 4-hydroxyphenyl phosphate (4-HPP) into HQ and the resultant HQ-controlled intense green Si-PDs generation, we have established a straightforward ALP activity assay by innovatively employing commercially available 4-HPP as the substrate. More significantly, the specific preparation method, clear formation mechanism and excellent performance enable the Si-PDs as well as its generation process to develop facile and attractive FL immunoassay. With the help of the universal ALP-based enzyme-linked immunosorbent assay (ELISA) platform and corresponding antibody, a convenient and conceptual ALP-based fluorescent ELISA has been constructed and applied in sensing cardiac troponin I (cTnI), a well-known biomarker of acute myocardial infarction. Our research via in situ formation of fluorescent nanomaterials has great potential application in ALP activity assay, inhibitor screening, and disease diagnosis.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2016YFA0201301), the National Natural Science Foundation of China (21435005, 21627808, 21974132), the Youth Innovation Promotion Association, CAS (2018258) and Open Project of State Key Laboratory of Supramolecular Structure and Materials (sklssm2019023).

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Guoyong Liu, Jiahui Zhao, Mengxia Yan, Shuyun Zhu, Jian Sun & Xiurong Yang

  2. University of Science and Technology of China, Hefei, 230026, China

    Guoyong Liu, Mengxia Yan & Xiurong Yang

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Jiahui Zhao

  4. College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, China

    Shuyun Zhu

  5. Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China

    Wenchao Dou

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  1. Guoyong Liu
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  2. Jiahui Zhao
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  3. Mengxia Yan
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  4. Shuyun Zhu
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  6. Jian Sun
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Correspondence to Jian Sun or Xiurong Yang.

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11426_2019_9690_MOESM1_ESM.doc

In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay

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Liu, G., Zhao, J., Yan, M. et al. In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay. Sci. China Chem. 63, 554–560 (2020). https://doi.org/10.1007/s11426-019-9690-7

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  • DOI: https://doi.org/10.1007/s11426-019-9690-7

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