Abstract
Co-immobilization of enzymes and microorganism is an effective way to enable cells to use nonmetabolizable substrates and accelerate reaction rate of overall process. Herein, a facile strategy to separately co-immobilize β-glucosidase (BG) and yeast cells on non-woven fabrics was developed. The BG was firstly in situ entrapped into poly(ethylene glycol) (PEG) network grafted on non-woven fabrics by visible light induced living/controlled graft polymerization. Then re-graft polymerization was performed on the as-formed BG loaded layer by taking advantage of living-grafting polymerization on its surface to in situ encapsulate yeast cells into the second PEG network layer. This layered structure of co-immobilization avoided possible interference between enzyme and cells. Viability assay of yeast cells demonstrated that most of cells were viable after immobilization. While immobilized BG showed decreased Vmax compared to free BG, indicating that entrapping BG into inner PEG network layer restricted its accessibility with substrates. This co-immobilization sheet could successfully convert cellobiose to ethanol and a maximum of 98.6% bioethanol yield can be obtained after 48 h of simultaneous saccharification and fermentation (SSF). The co-immobilization sheet showed excellent reusability and could still reach more than 60% of original ethanol yield after reusing for 7 batches. Compared with the mixed co-immobilization, the sequential layered immobilization in this system showed better stability and higher ethanol yield.
Similar content being viewed by others
References
Cannella D, Jørgensen H. Biotechnol Bioeng, 2014, 111: 59–68
Paulova L, Patakova P, Branska B, Rychtera M, Melzoch K. Biotech Adv, 2015, 33: 1091–1107
Sarkar N, Ghosh SK, Bannerjee S, Aikat K. Renew Energy, 2012, 37: 19–27
Meng X, Ragauskas AJ. Curr Opin Biotech, 2014, 27: 150–158
Alvira P, Tomás-Pejó E, Ballesteros M, Negro MJ. Bioresource Tech, 2010, 101: 4851–4861
Payne CM, Knott BC, Mayes HB, Hansson H, Himmel ME, Sandgren M, Ståhlberg J, Beckham GT. Chem Rev, 2015, 115: 1308–1448
Holtzapple M, Cognata M, Shu Y, Hendrickson C. Biotechnol Bioeng, 1990, 36: 275–287
Dekker RFH, Wallis AFA. Biotechnol Bioeng, 1983, 25: 3027–3048
Chauve M, Mathis H, Huc D, Casanave D, Monot F, Lopes Ferreira N. Biotechnol Biofuels, 2010, 3: 3–8
Teugjas H, Väljamäe P. Biotechnol Biofuels, 2013, 6: 104
Castro RCA, Roberto IC. Appl Biochem Biotechnol, 2014, 172: 1553–1564
Cao LC, Wang ZJ, Ren GH, Kong W, Li L, Xie W, Liu YH. Biotechnol Biofuels, 2015, 8: 202
Gupta VK, Kubicek CP, Berrin JG, Wilson DW, Couturier M, Berlin A, Filho EXF, Ezeji T. Trends Biochem Sci, 2016, 41: 633–645
Goldemberg J. Biotech Biofuels, 2008, 1: 6
Kossatz HL, Rose SH, Viljoen-Bloom M, van Zyl WH. Process Biochem, 2016, 53: 10–16
Saha BC, Nichols NN, Qureshi N, Kennedy GJ, Iten LB, Cotta MA. Bioresour Tech, 2015, 175: 17–22
de Barros EM, Carvalho VM, Rodrigues THS, Rocha MVP, Gonçalves LRB. Chem Eng J, 2016, 307: 939–947
Brethauer S, Robert Lawrence S, Michael Hans-Peter S. Bioresource Tech, 2017, 237: 135–138
Tran CTH, Nosworthy N, Bilek MMM, McKenzie DR. Biomass Bioenergy, 2015, 81: 234–241
Watanabe I, Miyata N, Ando A, Shiroma R, Tokuyasu K, Nakamura T. Bioresource Tech, 2012, 123: 695–698
Wirawan F, Cheng CL, Kao WC, Lee DJ, Chang JS. Appl Energy, 2012, 100: 19–26
Choi IS, Lee YG, Khanal SK, Park BJ, Bae HJ. Appl Energy, 2015, 140: 65–74
Karagöz P, Özkan M. Bioresource Tech, 2014, 158: 286–293
Chen CC, Wu CH, Wu JJ, Chiu CC, Wong CH, Tsai ML, Lin HTV. Process Biochem, 2015, 50: 1509–1515
Zhou Y, Pan S, Wei X, Wang L, Liu Y. Bioresources, 2013, 8: 2605–2619
Martino A, Pifferi PG, Spagna G. Process Biochem, 1996, 31: 287–293
Hahn-Hägerdal B. Biotechnol Bioeng, 1984, 26: 771–774
Grosová Z, Rosenberg M, Gdovin M, Sláviková L, Rebroš M. Food Chem, 2009, 116: 96–100
Staniszewski M, Kujawski W, Lewandowska M. J Food Eng, 2009, 91: 240–249
Giordano RLC, Trovati J, Schmidell W. Appl Biochem Biotechnol, 2008, 147: 47–61
Bandaru VVR, Somalanka SR, Mendu DR, Madicherla NR, Chityala A. Enzyme Microbial Tech, 2006, 38: 209–214
Zhu X, Ma Y, Zhao C, Lin Z, Zhang L, Chen R, Yang W. Langmuir, 2014, 30: 15229–15237
Bradford MM. Anal Biochem, 1976, 72: 248–254
Ma J, Luan S, Song L, Jin J, Yuan S, Yan S, Yang H, Shi H, Yin J. ACS Appl Mater Interfaces, 2014, 6: 1971–1978
Yan S, Luan S, Shi H, Xu X, Zhang J, Yuan S, Yang Y, Yin J. Biomacromolecules, 2016, 17: 1696–1704
Klis FM. Yeast, 1994, 10: 851–869
Figueira JA, Sato HH, Fernandes P. J Agric Food Chem, 2013, 61: 626–634
Albino Gomes A, Pazinatto Telli E, Miletti LC, Skoronski E, Gomes Ghislandi M, Felippe da Silva G, Borba Magalhães ML. Biotech Appl Biochem, 2018, 65: 246–254
Kazan A, Heymuth M, Karabulut D, Akay S, Yildiz-Ozturk E, Onbas R, Muderrisoglu C, Sargin S, Heils R, Smirnova I, Yesil-Celiktas O. Eng Life Sci, 2017, 17: 714–722
Carvalho Y, Almeida JMAR, Romano PN, Farrance K, Demma Carà P, Pereira N, Lopez-Sanchez JA, Sousa-Aguiar EF. Appl Biochem Biotechnol, 2017, 182: 1619–1629
Zhang L, Ma Y, Zhao C, He B, Zhu X, Yang W. Ind Eng Chem Res, 2016, 55: 6354–6364
Olofsson K, Bertilsson M, Lidén G. Biotechnol Biofuels, 2008, 1: 7
Acknowledgements
This work was supported by the National Natural Science Foundation of China (51521062, 51103009, 51473015), the Innovation and Promotion Project of Beijing University of Chemical Technology and the Beijing Natural Science Foundation (2162035).
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Rights and permissions
About this article
Cite this article
He, B., Zhu, X., Zhao, C. et al. Sequential co-immobilization of β-glucosidase and yeast cells on single polymer support for bioethanol production. Sci. China Chem. 61, 1600–1608 (2018). https://doi.org/10.1007/s11426-018-9319-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11426-018-9319-1