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Enhanced production of poly-3-hydroxybutyrate by recombinant Escherichia coli containing NAD kinase and phbCAB operon

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Abstract

With the help of Tn5 transposon technique, gene yfjB encoding NAD kinase in Escherichia coli (E. coli) was inserted into chromosome of recombinant E. coli polyhydroxybutyrate (PHB) containing PHB synthesis operon integrated in the host genome. After successful transposition of an extra yfjB gene copy into genome, the selected recombinant named E. coli PHBTY4 showed stronger NAD kinase activity than that of E. coli PHB. Shake flask studies suggested that both cell dry weight and PHB accumulation were significantly increased in E. coli PHBTY4 compared with that of the control. E. coli PHBTY4 produced approximately 23 g/L PHB compared with its control which synthesized only 10 g/L PHB when grown under the same conditions in a 6 L fermentor after 32 h of cultivation. In addition, E. coli PHBTY4 maintained high genetic stability during the cultivation processes. These results revealed a practical method to construct genetically stable strains harboring extra NAD kinase gene to enhance NADP(H)-dependent bio-reactions.

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Authors and Affiliations

  1. Center for Synthetic and Systems Biology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China

    Shaoqin Zhang, Lei Fang, Yingying Guo & Guo-Qiang Chen

  2. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Zhengjun Li

  3. Center for Nano and Micro Mechanics, Tsinghua University, Beijing, 100084, China

    Guo-Qiang Chen

Authors
  1. Shaoqin Zhang
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  2. Lei Fang
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  3. Zhengjun Li
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  4. Yingying Guo
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  5. Guo-Qiang Chen
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Corresponding authors

Correspondence to Yingying Guo or Guo-Qiang Chen.

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These authors contributed equally to this work.

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Zhang, S., Fang, L., Li, Z. et al. Enhanced production of poly-3-hydroxybutyrate by recombinant Escherichia coli containing NAD kinase and phbCAB operon. Sci. China Chem. 59, 1390–1396 (2016). https://doi.org/10.1007/s11426-016-0194-x

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  • DOI: https://doi.org/10.1007/s11426-016-0194-x

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