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Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides

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Abstract

This paper reports a simple method for immobilization of acetylcholinesterase (AChE) on one-dimensional (1D) gold (Au) nanoparticles for detection of organophosphorous (OP) insecticides. 1D Au nanoparticles were prepared by electrodeposition in the pores of an alumina template which was subsequently removed by 2.0 M NaOH solution. They were characterized by XRD and FESEM. The immobilized AChE retained its biological activity and catalyzed the hydrolysis of acetylthiocholine to form thiocholine, which was subsequently oxidized to produce detectable signals. Based on the inhibition toward the enzymatic activity of AChE by OP insecticides, sensitive detection of methamidophos (an OP insecticide) was performed. Under optimal conditions, the sensors could be used for the determination of methamidophos ranging from 0.004 to 24 μg/mL with the detection limit of 0.001 μg/mL. The developed OP insecticide biosensors exhibited satisfactory stability and reproducibility. This work demonstrated that 1D Au nanoparticles could serve as an ideal carrier for immobilization of AChE to fabricate the corresponding biosensor.

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biofunctional Materials, Nanjing Normal University, Nanjing, 210097, China

    YanRong Li, ZhiYong Gan, YanFen Li, Qian Liu, JianChun Bao, ZhiHui Dai & Min Han

  2. College of Chemistry and Environmental Science, Nanjing Normal University, Nanjing, 210097, China

    YanRong Li, ZhiYong Gan, YanFen Li, Qian Liu, JianChun Bao, ZhiHui Dai & Min Han

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  1. YanRong Li
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  2. ZhiYong Gan
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  3. YanFen Li
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  4. Qian Liu
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  5. JianChun Bao
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  6. ZhiHui Dai
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  7. Min Han
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Corresponding authors

Correspondence to ZhiHui Dai or Min Han.

Additional information

Support from the National Natural Science Foundation of China (Grant Nos. 20875046, 20871070 & 20901041).

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Li, Y., Gan, Z., Li, Y. et al. Immobilization of acetylcholinesterase on one-dimensional gold nanoparticles for detection of organophosphorous insecticides. Sci. China Chem. 53, 820–825 (2010). https://doi.org/10.1007/s11426-010-0105-0

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  • DOI: https://doi.org/10.1007/s11426-010-0105-0

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