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Ensuring traceability of organophosphate pesticides (OPs) through enzyme immobilized spheres

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Abstract

Currently, pesticide residues increasingly lead to health problems. Acetylcholinesterase enzyme (AChE) plays a role in detoxification processes due to their ability to scavenge organophosphates. Thus, this enzyme has been selected for the detection of pesticides. Herein, this study describes the preparation and application of appropriate new immobilized spheres that could be used for the detection of organophosphorus pesticide residues. That is a simple colorimetric enzymatic assay for the practical discovery of N-(Mercaptomethyl) phthalimide S–(O, O–dimethylphosphorodithioate) from organophosphorus pesticides (OPs) widely used in the treatment of sugar beet, apple, nut, corn, and tobacco. The process is based on the immobilization of acetylcholinesterase onto tryptophan FMPS-Trp, (FMPS-Trp)Pd(II), and (FMPS-Trp)Pt(II) functionalized spheres. Spectroscopic and microscopic techniques were used in the characterization of the spheres where scanning electron microscopy (SEM) is an essential tool. The (FMPS-Trp)Pt(II)-AChE showed a good affinity to acetylthiocholine chloride (ATCl) and was found to have the ability to catalyze the hydrolysis of ATCl with an apparent Michaelis–Menten constant value of 288 mM. The developed colorimetric process showed good qualitative analytical performance for Phosmet (OPs) detection as low as 10−7 M.

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Acknowledgement

This work was supported by the Gazi University Research Fund (05/2014-02).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Gazi University, 06500, Teknikokullar, Ankara, Turkey

    Elvan Hasanoğlu Özkan & Nurşen Sarı

  2. Department of Chemistry, Faculty of Arts and Science, Kırklareli University, Kırklareli, Turkey

    Nurdan Kurnaz Yetim

  3. Department of Polymer Engineering, Faculty of Technology, Düzce University, Düzce, Turkey

    Dilek Nartop

Authors
  1. Elvan Hasanoğlu Özkan
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  2. Nurdan Kurnaz Yetim
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  3. Dilek Nartop
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  4. Nurşen Sarı
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Contributions

Elvan Hasanoğlu Özkan contributed to formal analysis, investigation, methodology, writing-original draft, and visualization. Nurdan Kurnaz Yetim was involved in data curation and investigation. Dilek Nartop contributed to review and editing. Nurşen Sarı was involved in funding acquisition, supervision, and validation.

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Correspondence to Elvan Hasanoğlu Özkan.

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Hasanoğlu Özkan, E., Kurnaz Yetim, N., Nartop, D. et al. Ensuring traceability of organophosphate pesticides (OPs) through enzyme immobilized spheres. J IRAN CHEM SOC 18, 1749–1759 (2021). https://doi.org/10.1007/s13738-020-02147-y

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  • DOI: https://doi.org/10.1007/s13738-020-02147-y

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