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On-line electrochemical measurements of cerebral hypoxanthine of freely moving rats

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Abstract

This study demonstrates an on-line method for continuous measurements of cerebral hypoxanthine in the freely moving rats with integration of selective electrochemical biosensing with in vivo microdialysis sampling. The selective electrochemical biosensing is achieved by using xanthine oxidase (XOD) as the specific sensing element and Prussian blue (PB) as the electrocatalyst for the reduction of H2O2 generated from the oxidase-catalyzed reaction. The method is virtually interference-free from the coexisting electroactive species in the brain and exhibits a good stability and reproducibility. Upon integrated with in vivo microdialysis, the on-line method is well suitable for continuous measurements of cerebral hypoxanthine of freely moving rats, which is illustrated by the measurements of the microdialysates after the hypoxanthine standard was externally infused into the rat brain. This study essentially offers a facile on-line electrochemical approach to continuous measurements of cerebral hypoxanthine and could find some interesting applications in physiological and pathological investigations associated with hypoxanthine.

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    ZiPin Zhang, YuQing Lin & LanQun Mao

  2. Graduate Uniresity, Chinese Academy of Science, Beijing, China

    ZiPin Zhang

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  1. ZiPin Zhang
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  2. YuQing Lin
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  3. LanQun Mao
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Correspondence to LanQun Mao.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 20625515, 90813032, 20905071 & 20721140650), the National Basic Research Program of China (Grant No. 2007CB935603), and Chinese Academy of Sciences

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Zhang, Z., Lin, Y. & Mao, L. On-line electrochemical measurements of cerebral hypoxanthine of freely moving rats. Sci. China Ser. B-Chem. 52, 1677–1682 (2009). https://doi.org/10.1007/s11426-009-0231-8

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  • DOI: https://doi.org/10.1007/s11426-009-0231-8

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