Abstract
Horseradish peroxidase (HRP) and glucose oxidase (GOD) bienzyme biosensor was constructed by in-situ formation of the organic-inorganic biocomposite film based on the one-step electrodeposition and covalent-coupled sol-gel process. The electrodeposition was performed in the solution containing functional inorganic precursor possessing the epoxy groups, γ-glycidoxypropyltrimethoxysiloxane (GPTMS), a biopolymer chitosan (CS), HRP and GOD. The covalent-coupled sol-gel process was formed by self-hydrolysis and self-condensation of GPTMS, followed by in-situ covalent cross-linking of CS, HRP and GOD through covalent reaction between amino groups and epoxy groups. The developed bienzyme biosensor presented high stability in acidic solution owing to the covalent-coupled organic-inorganic hybridization. Compared with the non-hybrid HRP-GOD/CS/Au electrode, the bienzyme biosensor of HRP-GOD/GPTMS/CS/Au showed improved sensitivity and a wider linear range for the determination of glucose. The linear response of the developed HRP-GOD/GPTMS/CS/Au biosensor for the determination of glucose ranged from 1 to 351 μmol/L with a detection limit of 0.3 μmol/L.
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References
Coradin T, Allouche J, Boissiere M, Livage J. Sol-gel biopolymer/silica nanocomposites in biotechnology. Curr Nanosci, 2006, 2(3): 219–230
Muzzarelli C, Muzzarelli R A A. Natural and artificial chitosan-inorganic composites. J Inorg Biochem, 2002, 92(2): 89–94
Guibal E. Heterogeneous catalysis on chitosan-based materials: A review. Prog Polym Sci, 2005, 30(1): 71–109
Kaushik A, Khan R, Solanki P R, Pandey P, Alam J, Ahmad S, Malhotra B D. Iron oxide nanoparticles-chitosan composite based glucose biosensor. Biosens Bioelectron, 2008, 24(4): 676–683
Li F, Li X M, Zhang S S. One-pot preparation of silica-supported hybrid immobilized metal affinity adsorbent with macroporous surface based on surface imprinting coating technique combined with polysaccharide incorporated sol-gel process. J Chromatogr A, 2006, 1129(2): 223–230
Wang G H, Zhang L M. Using novel polysaccharide-silica hybrid material to construct an amperometric biosensor for hydrogen peroxide. J Phys Chem B, 2006, 110(49): 24864–24868
Abdullah J, Ahmad M, Heng L Y, Karuppiah N, Sidek H. Stacked films immobilization of MBTH in nafion/sol-gel silicate and horseradish peroxidase in chitosan for the determination of phenolic compounds. Anal Bioanal Chem, 2006, 386(5): 1285–1292
Tian X C, Li M J, Cai P X, Luo L J, Zou X Y. An amperometric cholesterol biosensor based on multiwalled carbon nanotubes and organically modified sol-gel/chitosan hybrid composite film. Anal Biochem, 2005, 337(1): 111–120
Liu Y L, Su Y H, Lai J Y. In situ crosslinking of chitosan and formation of chitosan-silica hybrid membranes with using γ-glycidoxypropyltrimethoxysilane as a crosslinking agent. Polymer, 2004, 45(20): 6831–6837
Liu Y L, Su Y H, Lee K R, Lai J Y. Crosslinked organic-inorganic hybrid chitosan membranes for pervaporation dehydration of isopropanol-water mixtures with a long-term stability. J Membr Sci, 2005, 251(1–2): 233–238
Shirosaki Y, Tsuru K, Hayakawa S, Osaka A, Lopes M A, Santos J D, Fernandes M H. In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes. Biomaterials, 2005, 26(5): 485–493
Xu J J, Luo X L, Du Y, Chen H Y. Application of MnO2 nanoparticles as an eliminator of ascorbate interference to amperometric glucose biosensors. Electrochem Commun, 2004, 6(11): 1169–1173
Zhao G, Xu J J, Chen H Y. Fabrication, characterization of Fe3O4 multilayer film and its application in promoting direct electron transfer of hemoglobin. Electrochem Commun, 2006, 8(1): 148–154
Xue M H, Xu Q, Zhou M, Zhu J J. In situ immobilization of glucose oxidase in chitosan-gold nanoparticle hybrid film on Prussian Blue modified electrode for high-sensitivity glucose detection. Electrochem Commun, 2006, 8(9): 1468–1474
Li F, Wang Z, Chen W, Zhang S S. A simple strategy for one-step construction of bienzyme biosensor by in-situ formation of biocomposite film through electrodeposition. Biosens Bioelectron, 2009, 24(10): 3030–3035
Yao Y L, Shiu K K. A mediator-free bienzyme amperometric biosensor based on horseradish peroxidase and glucose oxidase immobilized on carbon nanotube modified electrode. Electroanalysis, 2008, 20(19): 2090–2095
Zhu L, Yang R L, Zhai J L, Tian C Y. Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. Biosens Bioelectron, 2007, 23(4): 528–535
Jeykumari D R S, Narayanan S S. Fabrication of bienzyme nanobiocomposite electrode using functionalized carbon nanotubes for biosensing applications. Biosens Bioelectron, 2008, 23(11): 1686–1693
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Supported by the National Natural Science Foundation of China (Grant Nos. 20775039 & 20775038)
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Li, F., Wang, Z. & Feng, Y. Construction of bienzyme biosensors based on combination of the one-step electrodeposition and covalent-coupled sol-gel process. Sci. China Ser. B-Chem. 52, 2269–2274 (2009). https://doi.org/10.1007/s11426-009-0158-0
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DOI: https://doi.org/10.1007/s11426-009-0158-0