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Novel cyclen-based linear polymer as a high-affinity binding material for DNA condensation

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Abstract

A novel cyclen-based linear polyamine (POGEC) was designed and synthesized from the reaction between 1,3-propanediol diglycidyl ether and 1,7-bis(diethoxyphosphory)-1,4,7,10-tetraazacyclod- odecane. High-affinity binding between POGEC and DNA was demonstrated by agarose gel electrophoresis and scanning electron microscopy (SEM). Moreover, the formed POGEC/DNA complex (termed polyplex) could be disassociated to release the free DNA through addition of the physiological concentration of NaCl solution. Fluorescence spectrum was used to measure the high-affinity binding and DNA condensation capability of POGEC. Circular dichroism (CD) spectrum indicates that the DNA conformation did not change after binding to POEGC.

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Authors and Affiliations

  1. Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China

    YongZhe Xiang, Na Wang, Ji Zhang, Kun Li & XiaoQi Yu

  2. Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China

    ZhongWei Zhang & HongHui Lin

Authors
  1. YongZhe Xiang
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  2. Na Wang
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  3. Ji Zhang
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  4. Kun Li
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  5. ZhongWei Zhang
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  6. HongHui Lin
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  7. XiaoQi Yu
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Correspondence to XiaoQi Yu.

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Supported by the National Natural Science Foundation of China (Grant Nos. 20725206 and 20732004), Specialized Research Fund for the Doctoral Program of Higher Education, and Scientific Fund of Sichuan Province for Outstanding Young Scientist

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Xiang, Y., Wang, N., Zhang, J. et al. Novel cyclen-based linear polymer as a high-affinity binding material for DNA condensation. Sci. China Ser. B-Chem. 52, 483–488 (2009). https://doi.org/10.1007/s11426-009-0028-9

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  • DOI: https://doi.org/10.1007/s11426-009-0028-9

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