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Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis

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Abstract

Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate and N,N′-memylenebisacrylamid in the presence of trinary porogens, including 1,4-butanediol, dodecanol and dimethyl sulphoxide. With Ampholine immobilized on the monolith by chemical bonding according to their p/s, the monolithic immobilized pH gradient (M-IPG) was prepared, and applied to the separation of four standard proteins. Compared with polyacrylate based M-IPG, the hydrophilicity of the new material was improved. It could not only avoid the adsorption of proteins, but also make the synthesized procedure simple, which showed great potential in the analysis of proteins.

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Authors and Affiliations

  1. National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Zhu GuiJie, Zhang WeiBing, Zhang LiHua, Liang Zhen & Zhang YuKui

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Zhu GuiJie

Authors
  1. Zhu GuiJie
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  2. Zhang WeiBing
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  3. Zhang LiHua
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  4. Liang Zhen
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  5. Zhang YuKui
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Additional information

Supported by the State Key Fundamental Research Program (Grant No. 2001CB51020), National Natural Science Foundation of China (Grant Nos. 20405104 and 20435020) and 100 Talents Project of Chinese Academy of Sciences

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Zhu, G., Zhang, W., Zhang, L. et al. Preparation of polyacrylamide based monolith with immobilized pH gradient and its application for protein analysis. SCI CHINA SER B 50, 526–529 (2007). https://doi.org/10.1007/s11426-007-0063-3

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  • DOI: https://doi.org/10.1007/s11426-007-0063-3

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