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Direct electrochemistry and enzymatic activity of hemoglobin in positively charged colloid Au nanoparticles and hemoglobin layer-by-layer self-assembly films

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Abstract

Alternate adsorption of positively charged colloid-Au nanoparticles (nano-Au⊕) and negatively charged hemoglobin (Hb) on L-cysteine (L-cys) modified gold electrode resulted in the assembly of {Hb/nano-Au⊕}n layer-by-layer films/L-cys modified gold electrode. The nano-Au⊕ was characterized by transmission electron micrograph (TEM) and microelectrophoresis. The modified electrode interface morphology was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM), cyclic voltammograms (CV) and chronoamperometry. Direct electron transfer between hemoglobin and gold electrodes was studied, and the apparent Michaelis-Menten constant (k appm ) of the modified electrode was evaluated to be 0.10 mmol·L−1. Moreover, the higher activity of proteins in the nano-Au⊕ films could be retained compared with the electropolymerization membrane, since the proteins in nano-Au⊕ films retained their near-native structure. Direct electron transfer between hemoglobin and electrode and electrochemically catalyzed reduction of hydrogen peroxide on a modified electrode was studied, and the linear range was from 2.1×10−8 to 1.2×10−3 mol·L−1 (r=0.994) with a detection limit of 1.1×10−8 mol·L−1 H2O2.

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Authors and Affiliations

  1. Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Yuan Ruo, Cao ShuRui, Chai YaQin, Gao FengXian, Zhao Qing, Tang MingYu, Tong ZhongQiang & Xie Yi

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  1. Yuan Ruo
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  2. Cao ShuRui
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  3. Chai YaQin
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  4. Gao FengXian
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  5. Zhao Qing
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  6. Tang MingYu
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  7. Tong ZhongQiang
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  8. Xie Yi
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Correspondence to Yuan Ruo.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 20675064), the Natural Science Foundation of Chongqing City, China (Grant Nos. CSTC-2004 BB4149, 2005 BB4100) and High Technology Project Foundation of Southwest University (Grant No. XSGX02)

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Yuan, R., Cao, S., Chai, Y. et al. Direct electrochemistry and enzymatic activity of hemoglobin in positively charged colloid Au nanoparticles and hemoglobin layer-by-layer self-assembly films. SCI CHINA SER B 50, 620–628 (2007). https://doi.org/10.1007/s11426-007-0045-5

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  • DOI: https://doi.org/10.1007/s11426-007-0045-5

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