Abstract
Poria, the dried sclerotium of Wolfiporia cocos, is a medicinal mushroom that is widely used in traditional Japanese medicine. The fruit body of W. cocos is rarely found in the natural environment in Japan, therefore an optimized technique for fruit body formation is essential for producing new strains through crossbreeding and for biological research. Here, we developed a cultivation technique for fruit body formation of W. cocos using three strains collected from different areas of Japan. When mycelia were cultured on sawdust-based medium after liquid medium culture, all strains successfully formed fruit bodies as a brown honeycomb-like structure. Furthermore, we analyzed single nucleotide polymorphisms of the three strains using the STE3-like pheromone receptor protein gene, STE3.2, and found a genetic marker for discriminating one strain from the others. The results are expected to promote extensive studies on crossbreeding and domestic production of W. cocos.
References
Wang YZ, Zhang J, Zhao YL, Li T, Shen T, Li JQ, Li WY, Liu HG (2013) Mycology, cultivation, traditional uses, phytochemistry and pharmacology of Wolfiporia cocos (Schwein.) Ryvarden et Gilb.: a review. J Ethnopharmacol 147:265–276. https://doi.org/10.1016/j.jep.2013.03.027
Yamamoto Y, Kasahara R, Taira M, Takeda O, Higuchi Y, Yamaguchi Y, Shiratori M, Sasaki H (2021) Survey on crude drug usage in Japan (2). Shoyakugaku Zasshi 75:89–105
Kobira S, Atsumi T, Kakiuchi N, Mikage M (2012) Difference in cultivation characteristics and genetic polymorphism between Chinese and Japanese strains of Wolfiporia cocos Ryvarden et Gilbertson (Poria cocos Wolf). J Nat Med 66:493–499. https://doi.org/10.1007/s11418-011-0612-0
Mikage M, Tru A, Tsuda Y (1993) Studies on Fu-lin, Poria cocos Wolf (Polyporaceae). I. An ecological and morphological study of the Sclerotium. J Jpn Bot 68:114–121
Xu Z, Meng H, Xiong H, Bian Y (2014) Biological characteristics of teleomorph and optimized in vitro fruiting conditions of the Hoelen medicinal mushroom, Wolfiporia extensa (higher basidiomycetes). Int J Med Mushrooms 16:421–429. https://doi.org/10.1615/intjmedmushrooms.v16.i5.20
Xiang X, Wang X, Bian Y, Xu Z (2016) Development of crossbreeding high-yield-potential strains for commercial cultivation in the medicinal mushroom Wolfiporia cocos (higher basidiomycetes). J Nat Med 70:645–652. https://doi.org/10.1007/s11418-016-0995-z
Jo W-S, Lee S-H, Koo J et al (2017) Morphological characteristics of fruit bodies and basidiospores of Wolfiporia extensa. J Mushroom 15:54–56. https://doi.org/10.14480/JM.2017.15.1.54
Li S, Wang Q, Dong C (2021) Distinguishing homokaryons and heterokaryons in medicinal polypore mushroom Wolfiporia cocos (Agaricomycetes) based on cultural and genetic characteristics. Front Microbiol 11:3576. https://doi.org/10.3389/FMICB.2020.596715/BIBTEX
Tabata M, Hiraoka N (1994) Studies on the formation of fruit body and sclerotium in Poria cocos Wolf native to Japan. Nat Med 48:18–27
Atsumi T, Kakiuchi N, Mikage M (2007) DNA sequencing analysis of ITS and 28S rRNA of Poria cocos. Biol Pharm Bull 30:1472–1476. https://doi.org/10.1248/bpb.30.1472
James TY, Sun S, Li W, Heitman J, Kuo HC, Lee YH, Asiegbu FO, Olson A (2013) Polyporales genomes reveal the genetic architecture underlying tetrapolar and bipolar mating systems. Mycologia 105:1374–1390. https://doi.org/10.3852/13-162
Hu GS, Huang CG, Zhang Y, Xiao W, Jia JM (2017) Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats. Chin J Nat Med 15:265–270. https://doi.org/10.1016/S1875-5364(17)30043-2
Hu G, Zhai M, Niu R, Xu X, Liu Q, Jia J (2018) Optimization of culture condition for ganoderic acid production in Ganoderma lucidum liquid static culture and design of a suitable bioreactor. Molecules 23:2563. https://doi.org/10.3390/molecules23102563
Acknowledgements
We are grateful to Dr. Yohei Sasaki and Dr. Hirokazu Ando, Kanazawa University for kindly providing W. cocos mycelia. We also thank Dr. Masayuki Mikage and Dr. Yukihiro Nomura for providing useful information.
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Kitamura, M., Muramatsu, N., Yokogawa, T. et al. Fruit body formation and intra-species DNA polymorphism in Japanese Wolfiporia cocos strains. J Nat Med 76, 675–679 (2022). https://doi.org/10.1007/s11418-022-01617-2
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DOI: https://doi.org/10.1007/s11418-022-01617-2