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Isolation and evaluation of trypanocidal activity of sesquiterpenoids, flavonoids, and lignans in Artemisia sieversiana collected in Mongolia

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Abstract

Artemisia sieversiana is an annual herbaceous plant distributed throughout Central and East Eurasia and is regarded as an undesirable forage plant in Mongolia. It affects livestock, so information about its chemical composition is needed. We isolated three new sesquiterpenoids (1–3) and known compounds from A. sieversiana and investigated their activities. The absolute configuration of 1 was established using single-crystal X-ray diffraction crystallography, and its configuration differed from those of reported compounds with similar structures. Two additional new sesquiterpenoids (2 and 3) with similar structures were identified, and their configurations were determined. The trypanocidal activities of the isolated compounds (1–18) against Trypanosoma congolense and the pathogen responsible for fatal trypanosomosis in animals were estimated. Flavonoids and lignans were identified as active compounds with IC50 values ranging from 2.9 to 90.2 µM

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Acknowledgement

We thank Ms. S. Kasai, Mr. S. Sato, and Mr. T. Matsuki, Tohoku Medical and Pharmaceutical University, for assistance with the compound isolations and the MS measurements. This work was supported by the Consulting service project from the Ministry of Food, Agriculture and Light Industry of Mongolia (190812/04.294). This work was financed by a grant from JSPS Kakenhi (JP19K16397), JICA M-JEED project, JST/JICA SATREPS, AMED/JICA SATREPS, the Kanno Foundation of Japan, a Cooperative Research Grant (31-joint-5) from the National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine. The authors would like to thank Enago for the English language review.

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Authors and Affiliations

  1. National University of Mongolia, Ulaanbaatar, Mongolia

    Stipan Nurbek, Bekh-Ochir Davaapurev & Javzan Batkhuu

  2. Department of Pharmacognosy, Tohoku Medical and Pharmaceutical University, 4-1 Komatsushima 4-chome, Aoba-ku, Sendai, 981-8558, Japan

    Toshihiro Murata, Buyanmandakh Buyankhishig & Kenroh Sasaki

  3. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

    Keisuke Suganuma

  4. University of Shizuoka, Shizuoka, Japan

    Yoshinobu Ishikawa

  5. Rigaku Corporation, Tokyo, Japan

    Takashi Kikuchi

  6. Institute of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar, Mongolia

    Tseesuren Byambajav

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  1. Stipan Nurbek
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Nurbek, S., Murata, T., Suganuma, K. et al. Isolation and evaluation of trypanocidal activity of sesquiterpenoids, flavonoids, and lignans in Artemisia sieversiana collected in Mongolia. J Nat Med 74, 750–757 (2020). https://doi.org/10.1007/s11418-020-01429-2

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