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Triterpenes involved in the anti-inflammatory effect of ethanolic extract of Pterodon emarginatus Vogel stem bark

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Abstract

Bioassay-guided fractionation of the ethanolic extract of Pterodon emarginatus Vogel stem bark (EtEx) resulted in the isolation and characterization of lupeol and betulin. Their structures were elucidated by spectroscopic methods including IR, 1H-NMR, 13C-NMR and comparison with literature values. This study showed the anti-inflammatory activity of EtEx, the hexane (HexL) and dichloromethane (DichL) layers, and lupeol and betulin. The extract, HexL, DichL, lupeol and betulin were able to inhibit acetic acid-induced writhing. In the formalin test, EtEx decreased licking time only in the second phase characterizing anti-inflammatory activity. In the oil-induced ear oedema test, EtEx, lupeol and betulin decrease edema formation. In conclusion, EtEx has antinociceptive effects arising from anti-inflammatory activity; this activity could be due to the presence of lupeol and betulin.

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Acknowledgments

The authors are grateful to Mrs. Ekaterina A. F. B. Rivera and Jackson Nascimento de Lima for technical assistance. This research was supported by CAPES, CNPq/PIBIC, FAPEG and FUNAPE/UFG.

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  1. Pablinny Moreira Galdino

    Present address: Laboratório de Farmacologia de Produtos Naturais, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Sala 216, ICB 2, CP 131, Goiânia, GO, 74001-970, Brazil

Authors and Affiliations

  1. Laboratório de Pesquisa em Produtos Naturais, Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, GO, Brazil

    Weuller F. de Moraes, Maria Teresa F. Bara & José Realino de Paula

  2. Laboratório de Farmacologia de Produtos Naturais, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Sala 216, ICB 2, CP 131, Goiânia, GO, 74001-970, Brazil

    Marcus Vinícius M. Nascimento & Elson A. Costa

  3. Departamento de Ciências Fisiológicas, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil

    Frederico Argollo Vanderlinde

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  1. Weuller F. de Moraes
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  4. Frederico Argollo Vanderlinde
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  5. Maria Teresa F. Bara
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Correspondence to Pablinny Moreira Galdino.

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de Moraes, W.F., Galdino, P.M., Nascimento, M.V.M. et al. Triterpenes involved in the anti-inflammatory effect of ethanolic extract of Pterodon emarginatus Vogel stem bark. J Nat Med 66, 202–207 (2012). https://doi.org/10.1007/s11418-011-0547-5

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  • DOI: https://doi.org/10.1007/s11418-011-0547-5

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