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DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

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Abstract

The internal transcribed spacer 1 (ITS1) region between 18S and 5.8S rDNA was amplified from 25 crude drug specimens of So-jutsu (Atractylodes lancea rhizome) and 11 specimens of Byaku-jutsu (Atractylodes rhizome) obtained in the Chinese market. Based on the comparison of the nucleotide sequences of the PCR products with those determined in five Atractylodes species, we were able to identify the plant origins of these crude drugs. Of the 25 specimens of So-jutsu analyzed, 20 were identified to be derived from either A. chinensis, A. lancea or the interspecific hybrid between these two species, whereas five specimens were identified to be derived from A. japonica, i.e. Byaku-jutsu. In contrast, all of the specimens of Byaku-jutsu analyzed were derived from either A. ovata or A. japonica. A PCR-based method was established for the rapid identification and/or discrimination of So-jutsu and Byaku-jutsu without sequencing.

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Acknowledgements

The present investigation was supported by a grant from The Japan Health Sciences Foundation and, in part, by Sasakawa Scientific Research Grant (to Y.G.) from The Japan Science Society.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan

    Yahong Guo & Hajime Mizukami

  2. Pharmacognosy and Medicinal Resources Research Department, Central Research Laboratories, Tsumura & Co., Ibaraki, 300-119, Japan

    Kenji Kondo & Susumu Terabayashi

  3. Tochimoto tenkaido Co., Oniya, Kaibara, Tanba, Hyogo, 669-3315, Japan

    Yutaka Yamamoto & Hiroshi Shimada

  4. Uchida Wakanyaku Co., 4-4-1 Higashinippori, Arakawa-ku, Tokyo, 116-8571, Japan

    Masao Fujita & Takeshi Kawasaki

  5. Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku-Tokyo, 158-8501, Japan

    Takuro Maruyama & Yukihiro Goda

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  1. Yahong Guo
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  2. Kenji Kondo
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  3. Susumu Terabayashi
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  4. Yutaka Yamamoto
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  5. Hiroshi Shimada
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  6. Masao Fujita
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  7. Takeshi Kawasaki
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  8. Takuro Maruyama
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  9. Yukihiro Goda
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  10. Hajime Mizukami
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Correspondence to Hajime Mizukami.

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Guo, Y., Kondo, K., Terabayashi, S. et al. DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region . J Nat Med 60, 149–156 (2006). https://doi.org/10.1007/s11418-006-0032-8

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  • DOI: https://doi.org/10.1007/s11418-006-0032-8

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