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Biochars regulate bacterial community and their putative functions in the charosphere: a mesh-bag field study

  • Soils, Sec 1 • Soil Organic Matter Dynamics and Nutrient Cycling • Research Article
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A Correction to this article was published on 17 November 2022

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Abstract

Purpose

The term “charosphere” refers to the biochar-contiguous soil that is directly influenced by the physicochemical properties of the biochar, yet the dynamics of microbial composition in the charosphere in heavy metal-polluted soil remains largely unknown.

Methods

Swine manure-derived biochars prepared at 300 and 700 °C were packed in a double-layer mesh bag and then buried in a Cd-contaminated paddy soil for 15 days (T1) and 95 days (T2). Bacterial community composition in the charosphere and the linked properties such as soil pH, dissolved organic carbon (DOC) contents, and total Cd and Zn concentrations were analyzed.

Results

The results showed that biochar significantly shifted the beta diversity of the bacterial community in the charosphere, mainly due to increase in pH and total Zn concentrations. Further, 300 °C-prepared biochars stimulated Bacteroidetes and inhibited Acidobacteria by increasing soil pH. In the charosphere of biochars prepared at 700 °C, associated with the release of Zn and the adsorption of Cd, Chloroflexi became favorable over other microbial groups. Both biochar additions gave an increase in the putative function of energy metabolism and lipid metabolism, which might help bacteria dominant in charosphere resist stress of heavy metals.

Conclusion

Taken together, these pieces of evidence suggest that bacterial community and potential functions in the charosphere differ remarkably from the control soil without biochar, which was mainly due to biochar reduced environmental stresses such as soil acidity and heavy metals.

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Acknowledgements

This work was financially funded by the National Natural Science Foundation of China (42277282, 41601334), the Public Welfare Technology Application Research Project of Zhejiang Province, China (LGF21D010002), the Key Research and Development Program of Zhejiang Province, China (2020C01017, 2019C02053), Basic and Applied Basic Research Foundation of Guangdong Province (2022A1515010861), and Young Teachers Team Project of Fundamental Research Funds for the Central Universities, Sun Yat-sen University (Project no. 22qntd2702).

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Authors and Affiliations

  1. Zhejiang Province Key Laboratory of Recycling and Ecological Treatment of Waste Biomass, School of Environment and Natural Resources, Zhejiang University of Science and Technology, Hangzhou, 310023, China

    Jun Meng

  2. Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yan Li

  3. State Key Laboratory of Biocontrol, School of Ecology, Sun Yat-sen University, Guangzhou, 510006, China

    Yingbo Qiu & Huaihai Chen

  4. College of Environmental & Natural Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, 310058, China

    Yu Luo

  5. NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, 2568, Australia

    Yunying Fang

  6. Department of Primary Industries, New South Wales, 1243 Bruxner Highway, Wollongbar, NSW, 2477, Australia

    Lukas Van Zwieten

  7. Biochar Engineering Technology Research Center of Guangdong Province, Physical Science Public Platform, School of Environmental and Chemical Engineering, Foshan University, Foshan, 528000, Guangdong, China

    Hailong Wang

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  1. Jun Meng
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Meng, J., Li, Y., Qiu, Y. et al. Biochars regulate bacterial community and their putative functions in the charosphere: a mesh-bag field study. J Soils Sediments 23, 596–605 (2023). https://doi.org/10.1007/s11368-022-03362-1

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