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Ecoenzymatic stoichiometry and microbial nutrient limitation of shrub rhizosphere soils in response to arbuscular mycorrhizal fungi inoculation

  • Soils, Sec 4 • Ecotoxicology • Research Article
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Abstract

Purpose

The presence and interaction of shrubs and arbuscular mycorrhizal fungi (AMF) are important for the biogeochemical cycles and sustainable development of arid and semiarid ecosystems. The regulatory mechanisms of the soil microenvironment were explored by studying microbial nutrient constraints in ecosystems.

Materials and methods

Three native shrubs were planted with and without AMF inoculation in the experimental nursery of Inner Mongolia Agricultural University. The soil chemical properties; enzyme activities related to carbon (C), nitrogen (N), and phosphorus (P) acquisition; and microbial biomass C and N were measured to determine the ecoenzymatic stoichiometries and microbial nutrient limitations of the rhizosphere soils.

Results

The ratios of ecoenzymes associated with C acquisition in the rhizospheres of Spiraea pubescens were the largest, as well as microbial C limitation. In addition, Clematis fruticosa and Amygdalus mongolica inoculated with AMF significantly increased the ratios of enzymes related to C acquisition and increased microbial C limitation in the rhizospheres of these two shrubs. The ratio of N and P acquisition enzymes showed that A. mongolica and S. pubescens were significantly lower than C. fruticosa, and the lowest microbial P limitation was found in the rhizosphere soil of C. fruticosa in the noninoculated treatment; however, there was no significant difference between shrubs in the inoculated treatment. The microbial P limitation of the shrubs with AMF inoculation could be positive, negative, or not impacted. This result indicated that AMF regulated microbial P limitation in the rhizospheres of shrubs and was closely related to the accumulation of plant biomass. Partial least squares path model analysis indicated that the main influencing factors of microbial C and P limitation were glomalin-related soil protein (GRSP) and soil available nutrients (SANU).

Conclusions

The results indicated that shrubs and AMF altered the ecoenzymatic stoichiometry of the rhizosphere soils, thus affecting soil microbial nutrient limitation through GRSP and SANU. Native shrubs with AMF inoculation can regulate soil microbial metabolism, providing a theoretical basis for vegetation restoration of degraded ecosystems in drylands.

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Acknowledgements

We are grateful to the Ecological Restoration Laboratory of Heilongjiang University for providing the AMF inoculum.

Funding

This work was funded by the National Natural Science Foundation of China (41761055), the China Postdoctoral Science Foundation (2018M643778XB), and the Science and Technology Planning Project of Inner Mongolia (2020GG0029 and 2020GG0075).

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  1. Tingyan Liu and Longfei Hao are contributed equally to this work.

Authors and Affiliations

  1. College of Forestry, Inner Mongolia Agricultural University, Hohhot, 010019, China

    Tingyan Liu, Longfei Hao & Shulan Bai

  2. School of Economics and Management, Inner Mongolia University of Technology, Hohhot, 010051, China

    Yanlin Wang

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  1. Tingyan Liu
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  2. Longfei Hao
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Correspondence to Shulan Bai or Yanlin Wang.

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Liu, T., Hao, L., Bai, S. et al. Ecoenzymatic stoichiometry and microbial nutrient limitation of shrub rhizosphere soils in response to arbuscular mycorrhizal fungi inoculation. J Soils Sediments 22, 594–606 (2022). https://doi.org/10.1007/s11368-021-03096-6

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