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The bio-mitigation of acetochlor in soil using Rhodopseudomonas capsulata in effluent after wastewater treatment

  • Soils, Sec 3 • Remediation and Management of Contaminated or Degraded Lands • Research Article
  • Published:
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An Editorial Expression of Concern to this article was published on 28 November 2020

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Abstract

Purpose

The bio-mitigation of acetochlor and improvement of fertility in soil using Rhodopseudomonas capsulata (R. capsulata) in effluent was investigated in this research.

Materials and methods

Acetochlor content and cytochrome P450 monooxygenase activity were tested using the HPLC method. EthB gene regulation was measured by RT-PCR.

Results and discussion

It was observed that acetochlor was not degraded under the control group. The addition of effluent containing R. capsulata and residual organics degraded acetochlor efficiently and re-mediated soil fertility. Acetochlor mitigation reached 100% after 5 days under the optimal group (2000 mg/L). Interestingly, the acetochlor began to be degraded after day 1 of inoculation. Further research indicated that EthB gene was expressed after the first day of inoculation. Subsequently, the cytochrome P450 monooxygenase was synthesized to degrade acetochlor under EthB gene regulation. Analysis revealed that EthB and cytochrome P450 monooxygenase were inducible gene expressions and enzyme. The acetochlor as stimulus signal induced EthB expression through signal transduction pathway. This process took 1 day for R. capsulata, as they were ancient bacteria. However, the organics in soil and the control group were deficient, which could not maintain R. capsulata growth for over 1 day. The residual organics in effluent provided sufficient carbon sources and energy for R. capsulata under four effluent groups.

Conclusions

The new method completed the remediation of acetochlor pollution and the improvement of soil fertility and soybean processing wastewater treatment simultaneously, as well as realizing the resource reutilization of wastewater and R. capsulata as sludge.

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Change history

  • 28 November 2020

    An Editorial Expression of Concern to this paper has been published: https://doi.org/10.1007/s11368-020-02823-9.

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Funding

The study was supported by the National Nature Science Fund for Distinguished Young Scholars of China (Grant No. 41625002); the National Natural Science Foundation of China (Grant No. 31700432; 31470550; 81500493; 31400386; 51778114); Natural Science Foundation of Guangdong Province (Grant No. 2015A030313098); Application Technology Research and Development Projects of Harbin (Grant No. 2016RAXXJ103); and the MOA Modern Agricultural Talents Support Project for valuable financial support. Basic scientific research service fees of the Central University and Dalian Nationalities University (0113-20000101).

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Authors and Affiliations

  1. School of Resources and Environment, Key Laboratory of Soybean Biology in Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, China

    Pan Wu, Bo Cao, Ying Zhang, Wenbin Li, Yuan Wu & Ning Li

  2. School of Environment and Resources, Dalian University of Minzhu, Dalian, 116024, China

    Pan Wu, Ying Zhang, Yuan Wu & Ning Li

  3. Department of Anesthesiology, The third affiliated hospital of SunYat-Sen University, Guangzhou, 510630, China

    Yanling Wang

Authors
  1. Pan Wu
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  2. Bo Cao
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  3. Ying Zhang
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  4. Wenbin Li
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  5. Yanling Wang
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  6. Yuan Wu
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  7. Ning Li
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Correspondence to Bo Cao.

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Responsible editor: Yanzheng Gao

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Wu, P., Cao, B., Zhang, Y. et al. The bio-mitigation of acetochlor in soil using Rhodopseudomonas capsulata in effluent after wastewater treatment. J Soils Sediments 19, 2927–2933 (2019). https://doi.org/10.1007/s11368-018-2201-2

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  • DOI: https://doi.org/10.1007/s11368-018-2201-2

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