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Influx and concentration of triazine pesticides in the Amaterska cave system, Moravian Karst, Czech Republic

  • Sediments, Sec 2 • Physical and Biogeochemical Processes • Research Article
  • Published:
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Abstract

Purpose

The aim of this study was to detect three triazine pesticides and their metabolites in the drip water and the sediment of the Amaterska cave system. Diversity of the bacterial community in the sediment was also assessed, and the potential role of bacteria in degradation of these pesticides was evaluated.

Materials and methods

Triazines and their metabolites were analyzed in the soil, drip water, and sediment of the Amaterska cave system area in seven sampling sites (S1–S7) based on the above ground cover that included forest, permanent grassland, and agriculture cropland. The bacterial community in the cave sediments (S1–S6) was also analyzed using the Illumina sequencing of the V3 and V4 regions of 16S rDNA.

Results and discussion

Triazines were present in the soil and drip water in all sites below grassland and agricultural land but not under the forest area. Only atrazine metabolites were detected in the surface soil. In contrast, atrazine was detected in all cave sediments regardless of above ground cover, and this is likely due to the occasional alluvial influx. The overall prevalence of bacteria potentially capable of atrazine degradation in the cave sediment ranged from 13.4 to 64.0% of the entire bacterial community. The concentrations of atrazine in the cave sediment were 16 to 70 times higher than in those in drip water.

Conclusions

High concentrations of atrazine in the cave sediment indicate a slow degradation rate of triazines in the cave likely due to low temperatures and absence of photolysis. The main source of atrazine in the Amaterska cave system is likely not drip water but the alluvial influx. Bacteria potentially capable of triazine degradation in the cave sediment were detected; however, their role in this process remains to be investigated.

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References

  • Arias-Estévez M, López-Periago E, Martínez-Carballo E, Simal-Gándara J, Mejuto J-C, García-Río L (2008) The mobility and degradation of pesticides in soil and the pollution of groundwater resources. Agric Ecosyst Environ 132:247–260

    Article  Google Scholar 

  • Baldini JUL, McDermott F, Fairchild IJ (2006) Spatial variability in cave drip water hydrochemistry: implications for stalagmite paleoclimate records. Chem Geol 235:390–404

    Article  CAS  Google Scholar 

  • Brooks RW, Parker BC, Gruft H, Falkinham JO III (1984) Epidemiology of infection by non-tuberculous mycobacteria. V. Numbers in eastern United States soils and correlation with soil characteristics. Am Rev Respir Dis 130:630–633

    CAS  Google Scholar 

  • Caporaso JG, Kuczynski J, Stombaugh J et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336

    Article  CAS  Google Scholar 

  • Commission Decision (2004) 2004/248/EC concerning the non-inclusion of atrazine in Annex I to Council Directive 91/414/EEC and the withdrawal of authorisations for plant protection products containing this active substance

  • Dehghani M, Nasseri S, Amin S, Naddafee K, Taghavi M, Yunesian M, Maleky N (2007) Isolation and identification of atrazine-degrading bacteria from corn field soil in Fars province of Iran. Pak J Biol Sci 10:84–89

    Article  CAS  Google Scholar 

  • Devers M, El Azhari N, Kolic NU, Martin-Laurent F (2007) Detection and organization of atrazine-degrading genetic potential of seventeen bacterial isolates belonging to divergent taxa indicate a recent common origin of their catabolic functions. FEMS Microbiol Lett 273:78–86

    Article  CAS  Google Scholar 

  • Dong X, Sun H (2016) Effect of temperature and moisture on degradation of herbicide atrazine in agricultural soil. Int J Environ Agric Res 2:150–157

    Google Scholar 

  • Engbaek HC, Vergmann B, Weis Bentzon B (1967) The sodium lauryl sulphate method in culturing sputum for mycobacteria. Scand J Respir Dis 48:268–284

    Google Scholar 

  • Faimon J, Bodláková R, Pracný P, Hebelka J (2016) Transfer of climatic variables by dripwater: a case study from Kateřinská cave (Moravian karst). Environ Earth Sci 75:1151

    Article  Google Scholar 

  • Fan X, Song F (2014) Bioremediation of atrazine: recent advances and promises. J Soils Sediments 14:1727–1737

    Article  CAS  Google Scholar 

  • Hartland A, Fairchild IJ, Lead JR, Borsato A, Baker A, Frisia S, Baalousha M (2012) From soil to cave: transport of trace metals by natural organic matter in karst dripwaters. Chem Geol 304–305:68–82

    Article  Google Scholar 

  • Hrnčiarová T, Mackovčin P, Zvara I et al. (2009) Atlas of landscape of the Czech Republic. Ministerstvo životního prostředí ČR a Výzkumný ústav Silva Taroucy pro krajinu a okrasné zahradnictví, v.v.i., Praha

  • Iker BC, Kambesis P, Oehre SA, Groves C, Barton HA (2010) Microbial atrazine breakdown in a karst groundwater systém and its effect on ekosystém energetics. J Environ Qual 39:509–518

    Article  CAS  Google Scholar 

  • Kalkhoff SJ, Kolpin DW, Thurman EM, Ferrer I, Barcelo D (1998) Degradation of chloroacetanilide herbicides: the prevalence of sulfonic and oxanilic acid metabolites in Iowa groundwaters and surface waters. Environ Sci Technol 32:1738–1740

    Article  CAS  Google Scholar 

  • Kamas J, Bruthans J, Vysoka H, Kovařík M (2015) Range of horizontal transport and residence time of nitrate in a mature karst vadose zone. Int J Speleol 44:49–59

    Article  Google Scholar 

  • Kluge T, Riechelmann DFC, Wieser M, Spötl C, Sültenfuβ J, Schröder-Ritzrau A, Niggemann S, Aeschbach-Hertig W (2010) Dating cave drip water by tritium. J Hydrol 394:396–406

    Article  CAS  Google Scholar 

  • Kogovšek J, Petrič M (2013) Increase of vulnerability of karst aquifers due to leakage from landfills. Environ Earth Sci 70:901–912

    Article  Google Scholar 

  • Kolpin DW, Barbash JE, Gilliom RJ (1998) Occurrence of pesticides in shallow ground water of the United States: initial results from the water-quality assessment program. Environ Sci Technol 32:558–566

    Article  CAS  Google Scholar 

  • Kontchou CY, Gschwind N (1999) Biodegradation of s-triazine compounds by a slable mixed bacterial community. Ecotoxicol Environ Saf 43:47–56

    Article  CAS  Google Scholar 

  • Kruger EL, Somasundaram L, Kanwar RS, Coats JR (1993) Persistence and degradation of [C-14] atrazine and [C-14] deisopropylatrazine as affected by soil depth and moisture conditions. Environ Toxicol Chem 12:1956–1967

    Article  Google Scholar 

  • Loos R, Locoro G, Comero S, Contini S, Schwesig D, Werres F, Balsaa P, Gans O, Weiss S, Blaha L, Bolchi M, Gawlik BM (2010) Pan-European survey on the occurrence of selected polar organic persistent pollutants in ground water. Water Res 44:4115–4126

    Article  CAS  Google Scholar 

  • Mahler BJ, Personne JC, Lods GF, Drogue C (2000) Transport of free and particulate-associated bacteria in karst. J Hydrol 238:179–193

    Article  Google Scholar 

  • Modra H, Bartos M, Hribova P, Ulmann V, Hubelova D, Konecny O, Gersl M, Kudelka J, Voros D, Pavlik I (2017) Mycobacteria in the Moravian karst environment (bull rock cave and the relevant water catchment area): the impact of water sediments, earthworm castings and bat faeces. Vet Med-Czech 62:153–168

    Article  Google Scholar 

  • Monard C, Vandenkoornhuyse P, Le Bot B, Binet F (2011) Relationship between bacterial diversity and function under biotic control: the soil pesticide degraders as a case study. ISME J 5:1048–1056

    Article  CAS  Google Scholar 

  • Nossa CW, Oberdorf WE, Yang L, Aas JA, Paster BJ, Desantis TZ, Brodie EL, Malamud D, Poles MA, Pei Z (2010) Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome. World J Gastroenterol 16:4135–4144

    Article  CAS  Google Scholar 

  • Parashar D, Chauhan DS, Sharma VD, Chauhan A, Chauhan SVS, Katoch VM (2004) Optimalization of procedures for isolation of mycobacteria from soil and water samples obtained in northern India. Appl Environ Microbiol 70:3751–3753

    Article  CAS  Google Scholar 

  • Perrin J, Jeannin PY, Zwahlen F (2003) Epikarst storage in a karst aquifer: a conceptual model based on isotopic data, Milandre test site, Switzerland. J Hydrol 279:106–124

    Article  CAS  Google Scholar 

  • Portaels F, De Muynck A, Sylla MP (1988) Selective isolation of mycobacteria from soil: a statistical analysis approach. J Gen Microbiol 134:849–855

    CAS  Google Scholar 

  • Pronk M, Goldscheider N, Zopfi J, Zwahlen F (2009) Percolation and particle transport in the unsaturated zone of a karst aquifer. Ground Water 47:361–369

    Article  CAS  Google Scholar 

  • Radosevich M, Martin EC, Ghosh D, Roy K, Peacock A, White DC (2006) In situ enrichment with BioSep beads yields diverse and metabolically novel atrazine degrading soil bacteria. The ASA-CSSA-SSSA International Annual Meetings

  • Roberts T (ed) (1998) Metabolic pathways of agrochemicals. Part one. Herbicides and plant growth regulators. The Royal Society of Chemistry, Cambridge

    Google Scholar 

  • Rousseaux S, Hartmann A, Soulas G (2001) Isolation and characterisation of new gram-negative and gram-positive atrazine degrading bacteria from different French soils. FEMS Microbiol Ecol 36:211–222

    Article  CAS  Google Scholar 

  • Schwartz K, Barth JAC, Postigo-Rebollo C, Grathwohl P (2009) Mixing and transport of water in a karst catchment: a case study from precipitation via seepage to spring. Hydrol Earth Syst Sci 13:285–292

    Article  Google Scholar 

  • Shabarova T, Villiger J, Morenkov O, Niggemann J, Dittmar T, Pernthaler J (2014) Bacterial community structure and dissolved organic matter in repeatedly flooded subsurface karst water pools. FEMS Microbiol Ecol 89:111–126

    Article  CAS  Google Scholar 

  • Sheng G, Johnston CT, Teppen BJ, Boyd SA (2001) Potential contribution of smectite clays and organic matter to pesticide retention in soils. J Agric Food Chem 49:2899–2907

    Article  CAS  Google Scholar 

  • Smith D, Alvey S, Crowley DE (2005) Cooperative catabolic pathways within an atrazine-degrading enrichment culture isolated from soil. FEMS Microbiol Ecol 53:265–273

    Article  CAS  Google Scholar 

  • Trček B (2007) How can the epikarst zone influence the karst aquifer behaviour? Environ Geol 51:761–765

    Google Scholar 

  • Ulmann V, Kracalikova A, Dziedzinska R (2015) Mycobacteria in water used for personal hygiene in heavy industry and collieries: a potential risk for employees. Int J Environ Res Public Health 12:2870–2877

    Article  Google Scholar 

  • Wiliams PW (2008) The role of epikarst in karst and cave hydrogeology: a review. Int J Speleol 37:1–10

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the Czech Science Foundation (No. 16-13231S). We thank prof. Ludek Zurek from K-State University for English improvement and commenting on a former version of the manuscript.

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Authors and Affiliations

  1. Faculty of Regional Development and International Studies, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Helena Modrá, Ondřej Konečný & Ivo Pavlík

  2. Nature Conservation Agency of the Czech Republic, Svitavská 29, 678 01, Blansko, Czech Republic

    Eva Gruberová & Antonín Tůma

  3. Public Health Institute Ostrava, Partyzánské nám. 7, 702 00, Ostrava, Czech Republic

    Vít Ulmann

  4. Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackého 1, 612 42, Brno, Czech Republic

    Petra Kaucká

  5. Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    Markéta Vlková

  6. ALS Czech Republic Ltd, Na Harfě 336/9, 190 00, Praha, Czech Republic

    Taťana Halešová

  7. Faculty of AgriScience, Mendel University in Brno, Zemědělská 1, 613 00, Brno, Czech Republic

    Jan Kudělka & Milan Geršl

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  1. Helena Modrá
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  2. Eva Gruberová
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Correspondence to Helena Modrá.

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Responsible editor: Jan Schwarzbauer

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Modrá, H., Gruberová, E., Konečný, O. et al. Influx and concentration of triazine pesticides in the Amaterska cave system, Moravian Karst, Czech Republic. J Soils Sediments 18, 640–647 (2018). https://doi.org/10.1007/s11368-017-1831-0

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  • DOI: https://doi.org/10.1007/s11368-017-1831-0

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