Effects of saline water irrigation and fertilization regimes on soil microbial metabolic activity
- 593 Downloads
Irrigation and fertilization can change soil environment, which thereby influence soil microbial metabolic activity (MMA). How to alleviate the adverse effects by taking judicious saline water irrigation and fertilization regimes is mainly concerned in this research.
Materials and methods
Here, we conducted a field orthogonal designed test under different saline water irrigation amount, water salinity, and nitrogen fertilizer application. The metabolic profiles of soil microbial communities were analyzed by using the Biolog method.
Results and discussion
The results demonstrated that irrigation amount and fertilizer application could significantly change MMA while irrigation water salinity had no significant effect on it. Medium irrigation amount (30 mm), least (50 kg ha−1) or medium (350 kg ha−1) N fertilizer application, and whatever irrigation water salinity could obtain the optimal MMA. Different utilization rates of carbohydrates, amino acids, carboxylic acids, and polymers by soil microbial communities caused the differences of the effects, and D-galactonic acid γ-lactone, L-arginine, L-asparagine, D-glucosaminic acid, Tween 80, L-threonine, and D-galacturonic acid were the indicator for distinguishing the effects.
The results presented here demonstrated that by regulating irrigation water amount and fertilizer application, the effects of irrigation salinity on MMA could be alleviated, which offered an efficient approach for guiding saline water irrigation.
KeywordsBiolog Fertilization Saline water irrigation Soil microbial activity
This research was funded by the National Natural Science Foundation of China (41501113, 41471210, 31270482), Foundation for Excellent Youth Scholars of CAREERI, CAS (Y451051001), China Postdoctoral Science Foundation (2013M542407, 2014T70950), and International Postdoctoral Exchange Fellowship Program.
- Chen LJ, Feng Q, Li FR, Li CS (2014) A bidirectional model for simulating soil water flow and salt transport under mulched drip irrigation with saline water. Agr water. Manage 146:24–33Google Scholar
- Dingjian C, Qing S, Baoquan X, Limei P, Yan H (2011) Orthogonal test design for optimization of the extraction of flavonid from the fructus gardeniae. Biomed Environ Sci 24:688–693Google Scholar
- Garland JL, Mills AL (1991) Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl Environ Microbiol 57:2351–2359Google Scholar
- Kang YH (1998) Microirrigation for the development of sustainable agriculture. T CSAE 14:251–255 in Chinese with English abstractGoogle Scholar
- Li H, He HJ, Li TF, Zhang ZK (2014) Microbial activity and functional diversity in rhizosphere of cucumber under different subsurface drip irrigation scheduling. Chin J Appl Ecol 25:2349–2354 in Chinese with English abstractGoogle Scholar
- Min W, Hou ZA, Ye J, Ma LJ, Cao Z, Luo HL (2014) Soil microbial activity and community functional diversity in cotton field under long-term drip irrigation with saline water. Chin J Ecol 33:2950–2958 in Chinese with English abstractGoogle Scholar
- Pereira LS, Cordey I, Iacovides I (2002) Coping with water scarcity. Unesco, ParisGoogle Scholar
- Tyree MC, Seiler JR, Aust WM, Sampson DA, Fox TR (2006) Long-term effects of site preparation and fertilization on total soil CO2 efflux and heterotrophic respiration in a 33-year-old Pinus taeda L. Plantation on the wet flats of the Virginia lower coastal plain. Forest Ecol Manag 234:363–369CrossRefGoogle Scholar
- Weber KP, Legge RL (2010) Community-level physiological profiling. In: Cummings SP (ed) Methods in molecular biology: bioremediation. The Humana Press Inc., New Jersey, pp. 263–281Google Scholar