Abstract
We used microbiology and molecular biology techniques to screen out high-temperature and low-temperature-resistant saprobiotics for compost and prepared a compound fermentation bacteria agent to rapidly ferment cattle manure into high-quality organic fertilizer in low-temperature season. Conventional composting and high-throughput techniques were used to analyze the changes of physical and chemical indexes and biodiversity in the process of composting, from which high and low-temperature-resistant strains were obtained, and high-temperature and low-temperature-resistant solid composite bactericides were prepared and added to composting to verify the effects of composite bactericides on composting. The conventional composting cycle took 22 days, and the diversity of microflora increased first and then decreased. Composting temperature and microbial population were the key factors for the success or failure of composting. Two strains of high-temperature-resistant bacteria and six strains of low-temperature-resistant bacteria were screened out, and they were efficient in degrading starch, cellulose, and protein. The high-temperature and low-temperature-resistant solid bacterial agent was successfully prepared with adjuvant. The preparation could make the compost temperature rise quickly at low temperature, the high temperature lasted for a long time, the water content, C/N, and organic matter fell quickly, the contents of total phosphorus and total potassium were increased, and the seed germination index was significantly improved. Improve the composting effect. The solid composite bacterial agent can shorten the composting time at low temperature and improve the composting efficiency and quality.
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Funding
The study was supported by the Hebei Provincial Science and Technology Key Projects (22327303D).
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Tao Peng: conceptualization, methodology, data curation, formal analysis, investigation, visualization, writing—original draft. Shilin Yue: methodology, investigation, visualization. Wenshuai Mao: review and editing. Qing Yang: review and editing, resources. Guojun Jiang: conceptualization, methodology, review and editing, supervision, project administration, resources.
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Highlights
(1) The high-temperature-resistant bacteria Streptococcus thermophilus and Bacillus licheniformis P8-B2 and low-temperature-resistant bacteria Bacillus licheniformis, Bacillus giant, Bacillus cereus, and Bacillus thuringiensis were screened to degrade starch, cellulose, and protein efficiently.
(2) The high and low-temperature-resistant compost fermentation solid microbial composite agent was successfully prepared. The preparation can make the compost temperature rise quickly, the high-temperature duration is long, the water content, C/N, and organic matter decrease quickly, improve the total phosphorus and total potassium content and seed germination index, shorten the composting time, and improve the composting efficiency.
(3) High-throughput sequencing found that composting temperature and microbial quantity were the key factors affecting composting, resulting in large differences in microbial composition and abundance at different stages.
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Peng, T., Yue, S., Mao, W. et al. Preparation of high-temperature and low-temperature-resistant solid microbial agent for cattle manure fermentation and effect on composting. Environ Sci Pollut Res 31, 29017–29032 (2024). https://doi.org/10.1007/s11356-024-32830-z
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DOI: https://doi.org/10.1007/s11356-024-32830-z