Abstract
Aha Lake artificial reservoir wetland, Niangniang Mountain karst mountain wetland, and Caohai plateau lake wetland are typical karst wetlands in Guizhou Province with unique topography and geomorphic features. They were selected as research objects in this study to explore microorganisms and functional genes in nitrogen metabolism adopting macro-genome sequencing technology. It was found that Proteobacteria, Actinobacteria, and Acidobacteria were the dominant phyla in nitrogen metabolism in these three wetlands, similar to previous studies. However, at the genus level, there was a significant difference, with the dominant bacteria being Bradyrhizobium, Methylocystis, and Anaeromyxobacter. Six nitrogen metabolism pathways, including nitrogen fixation, nitrification, denitrification, dissimilatory nitrate reduction, assimilatory nitrate reduction, and complete nitrification, comammox, were revealed, but anaerobic ammonia oxidation genes were not detected. Nitrogen metabolism microorganisms and pathways were more affected by SOM, pH, NO3−, and EC in karst wetlands. This study further discussed microorganisms and functions of nitrogen metabolism in karst wetlands, which was of great significance to nitrogen cycles of karst wetland ecosystems.
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Funding
This work was financially supported by the National Natural Science Foundation of China (Grant No. 42267064), Science and Technology Plan Project of Guiyang Municipality (NO. [2024]3–38), and Science and Technology Innovation Team of Education Agency in Guizhou Province (Qian Jiao Ji [2023]056).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Chen Chen, Jia Ai, and Xin Tang. The first draft of the manuscript was written by Chen Chen. The review and edit were performed by Li Chen and Yancheng Li. The proofreading was performed by Yancheng Li and Jiang Li. All authors read and approved the final manuscript.
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Chen, C., Ai, J., Chen, L. et al. Nitrogen metabolism pathways and functional microorganisms in typical karst wetlands. Environ Sci Pollut Res 31, 22494–22506 (2024). https://doi.org/10.1007/s11356-024-32587-5
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DOI: https://doi.org/10.1007/s11356-024-32587-5