Abstract
The aims of this study were to investigate the presence, possible sources, and potential ecological risks of synthetic musk fragrances in freshwaters and sediments of the main tributaries of a deep subalpine lake in Northern Italy. The total musk concentrations ranged from few ng L−1 up to values > 500 ng L−1, depending on river characteristics: water flow and the presence of wastewater effluents proved to be the main factors affecting fragrance concentrations. The water flow may indeed dilute fragrance input mainly deriving from treated wastewaters. Good correlations (determination coefficients > 0.60) between synthetic fragrances concentrations and parameters related to anthropogenic impacts confirmed this hypothesis: synthetic fragrances were mainly detected in most polluted rivers crossing urbanized areas. Sediment analysis highlighted accumulation of fragrances in this matrix. Concentrations of synthetic fragrances up to 329 ng g−1 organic carbon were measured in sediments of the most contaminated rivers Boesio and Bardello, which also show the highest nutrient content. The preliminary environmental risk assessment revealed that present levels of synthetic musk fragrances do not pose any risk to the studied environmental compartments. However, a probable medium risk level was evidenced during the dry season in the most contaminated rivers Boesio and Bardello. For these reasons, small rivers draining urbanized watersheds and affected by wastewater effluents should be considered synthetic musk contamination hotspots that warrant further research.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Synthetic musks are a class of organic chemicals produced in large quantities and widely used in various consumer products as cosmetics, personal care, and household products (Homem et al. 2016; Vimalkumar et al. 2021). These chemicals have raised significant concern in recent years due to their potential threat to the environment and the human health (Fromme et al. 2001; Ramos et al. 2015). Between synthetic musks, polycyclic musks (PCMs) as Galaxolide® (1,3,4,6,7,8‑hexahydro‑4,6,6,7,8,8‑hexamethyl‑cyclopenta‑γ‑2‑benzopyran, or HHCB), Tonalide® (6-Acetyl-1,1,2,4,4,7-hexamethyltetralin, or AHTN), and, in minor part, Celestolide® (4-acetyl-6-tert-butyl-1,1-dimethylindan, or ADBI) and Phantolide® (5-acetyl-1,1,2,3,3,6-hexamethylindan, or AHMI) represent the 95% of the total fragrance market worldwide (European Commission 2008a, b). HHCB and AHTN production alone has been estimated about 1 million pounds per year since these compounds are the most widely employed worldwide and, for this reason, they were inserted on the High Production Volume List by the USEPA (Peck et al. 2006). They are compounds with acetylated and highly methylated pyran, tetralin, and indane skeletons, characterized by low production costs and a widespread availability that allowed a possible increase in the future in their global production and consumption (Hua et al. 2022). Even if they are structurally stable and relatively recalcitrant to degradation (Artola-Garicano et al. 2003), biotic and abiotic processes in the environment as enzymatic reactions and photodegradation can generate synthetic musks metabolites and transformation products. Between them, the most commonly found in the environment due to its concentration and stability is HHCB-lactone (HHCB-L), the main metabolite of HHCB (Bester 2004). This compound is generated mainly in wastewater treatment plants (WWTPs) by biological degradation in activated sludge (Tasselli et al. 2021) and during the ozone treatment (Herrera López et al. 2013). Once generated, this compound is more stable and recalcitrant to degradation respect to HHCB and, because of its higher polarity, it can be measured mainly in the aqueous matrix (Bester 2005).
Since PCMs are products directly applied on the human skin, they are not subjected to metabolic alterations during their employment; therefore, with a regular use, large quantity of these compounds may enter unaltered into the environment (Ternes et al. 2004). Synthetic musks are ubiquitous in the environment but they can be mainly detected in aquatic systems (Peck et al. 2006; Upadhyay et al. 2011; Weinberg et al. 2011). In fact, they can enter the aquatic environment directly, for example, through recreational activities in beaches and rivers as swimming and bathing, but also through indirect discharges of WWTP effluents since current wastewater treatments cannot completely remove these contaminants (Villa et al. 2020). Their release and presence with consequent potential bioaccumulation vary between regions and states according to the different usages (Hong et al. 2021). Based on the available literature, PCMs in surface water spread a wide range of concentrations from few ng L−1 to several tens of ng L−1 (Zhang et al. 2008; Sumner et al. 2010; Wang and Kelly 2017), meanwhile some sampling sites registering concentrations over µg L−1 (Fromme et al. 2001; Lee et al. 2010). Higher concentrations of PCMs in surface waters were generally registered in river systems located in anthropized area or subjected to discharges from WWTPs (Zhang et al. 2020).
Due to their physicochemical properties, synthetic musks are lipophilic, persistent, and highly bioaccumulative compounds. They may be easily adsorbed onto organic matter and, in aquatic ecosystems, accumulated in sediments and organisms (Kannan et al. 2005; Subedi et al. 2014; Huang et al. 2016; Vimalkumar et al. 2021). It has been highlighted that sediments are the final sinks for synthetic musks since field-derived log KOC (organic carbon–water partition coefficient) values for HHCB and AHTN are in the range of 3.86–4.86 (Fooken 2004; Wang et al. 2018). In fact, once release in the aquatic environment, PCMs can be removed primarily by outflowing wastewaters or losses into the atmosphere, while in summer direct photolysis is the main elimination process especially for AHTN (Buerge et al. 2003). Available literature reports the presence of polycyclic musk in different matrices as wastewater and sludge samples (Sun et al. 2014; Tasselli and Guzzella 2020) but also in surface waters and sediments (Villa et al. 2012; Wang et al. 2018). HHCB and AHTN were observed also in fish and other aquatic organisms especially in water bodies located near urban centers and anthropized areas (Zhang et al. 2013; Lange et al. 2015).
Various toxic effects related to synthetic musks were already highlighted, together with their potential action as endocrine disrupter chemicals (Tumová et al. 2019). Based on published data, polycyclic musks may be considered toxic from ppb to low ppm levels to aquatic invertebrates which, for longer exposure periods, appear to be more sensitive than fish (Brausch and Rand 2011). From these considerations, an assessment of the presence and behavior of synthetic musks in aquatic environments is still crucial, especially in Italy were data regarding PCM concentration in the environment are very scarce, despite Italy was the EU-Member State with the highest detergent consumption according to the last available survey performed by the International Association for Soaps, Detergents and Maintenance Products A.I.S.E. (HERA 2008). To start filling this gap of knowledge, in this study the presence of polycyclic musk fragrances was assessed for the first time in surface waters and sediments of the main tributaries of a deep subalpine lake in Northern Italy. Besides this, sample compound composition was considered to evaluate possible sources of PCMs, We also assessed the possible relationships between these pollutants and selected physical and chemical parameters of the considered rivers. Ultimately, potential ecological risk of synthetic musks for living aquatic organisms was evaluated according to the measured concentration levels.
Materials and methods
Study area
This study was carried out on Lake Maggiore, a deep subalpine lake belonging to the LTER (Long-Term Ecological Research) network (site EU-IT08-001-A). It is the second largest and the second deepest lake in Italy, with a surface area of 213 km2, a volume of 38 km3 and a maximum depth of 370 m. The catchment area is equal to 6599 km2 and, administratively, the area falls within the borders of Italy (3229 km2; Piedmont and Lombardy regions) and Switzerland (3370 km2; Canton Ticino), even if 80% of the lake surface is in the Italian territory. Studies on Lake Maggiore and its tributaries have been performed continuously since the 1980s in the framework of the limnological campaigns funded by the International Commission for the Protection of Italian-Swiss Waters (CIPAIS; www.cipais.org). Lake Maggiore is a holo-oligomictic lake as it rarely undergoes complete mixing (Guilizzoni et al. 2012), with a theoretical water renewal time of 4.5 years (Ambrosetti et al. 2012). Several other lakes are included in the Lake Maggiore watershed as lakes Orta, Lugano, and Varese. The lake watershed is prevalently occupied by mountains with low population density, apart from the shoreline area where towns like Varese, Lugano, Bellinzona, and Verbania are located. The total number of the inhabitants living in the Lake Maggiore area is 640,000, approximately. Especially in summer, tourism accounts for more than 300,000 additional equivalent inhabitants. Lake Maggiore hydrographic basin has Ticino Tributary, Toce, and Tresa as the main tributaries, while the only emissary is the Ticino River. Among other minor tributaries, considered in this work, there are Boesio River, which flows into the lake at Laveno-Mombello, Bardello River, emissary of Lake Varese, and Margorabbia River, which flows into Tresa River before entering the lake near the city of Luino. The coastal water environment is directly influenced by the contributions of tributaries and discharges into the lake; in fact, the quality of coastal waters can be very different from that of the pelagic zone. Different types of industries are located in this area which, together with urban WWTPs, can create serious problems in the absence of a correct and effective water management (Marziali et al. 2021).
Sampling design
In this study, water and sediment were sampled at the rivers’ mouth of Ticino Tributary, Toce, Tresa, Margorabbia, Boesio, Bardello, and Ticino Emissary (Fig. 1). Instantaneous water samples were taken monthly almost at the same time of the day, from April, 2021 to March, 2022, while sediments were sampled three times during the year (n = 3), in July and October 2021 and April 2022. The pluviometric regime in Lake Maggiore area is usually characterized by two maxima in spring and autumn and two minima in winter and summer (Saidi et al. 2013). However, within the study period (2021–2022), precipitation showed a quite distinct pattern, with higher than average precipitation (and consequently river discharge) in July, 2021, and very scarce precipitation during the first part of 2022. As a consequence sediment sampling covered sharply different hydrological conditions, from very wet in July, 2021 (mean precipitation 335 mm with respect to the long-term average 1978–2020 for this month of 132 mm) to moderately dry in April, 2022 (99 mm, with respect to 164 mm) (CNR IRSA 2022, 2023).
Synthetic musk fragrances were measured in instantaneous freshwater samples of Lake Maggiore main tributaries every month for 1 year (n = 12). During the first 6 months, from April to September, 2021, fragrances were measured in all the seven main tributaries while, from October, 2021, to April, 2022, the analysis of fragrances was not carried out in Ticino Emissary and Toce rivers. Water samples were taken using a bucket and water was then stored in 2.5-L amber glass bottle, previously washed with acetone, and preserved at 4° C until analyses for polycyclic musk, carried out the day after samplings. One liter of water was also collected in polyethylene bottles for chemical analyses which were completed within 3 days after the sampling. All materials were previously rinsed with the respective river water before use.
For sediments, depositional areas were identified at each site, and subsamples of freshly deposited sediments (5–10 cm depth) were collected using a stainless-steel spoon and mixed to obtain a 2-L representative sample. Sediments were preserved in dark glass bottles at 4 °C until freeze-drying within 1 week from sampling. Dry sediments were then sieved to separate the finest fraction (< 63 µm grain size), in which organic pollutants usually accumulate, for chemical analyses (US EPA 2001; OSPAR 2002).
Chemicals
Polycyclic musk standards HHCB (purity 95%), HHCB-L (purity 97%), AHTN (purity 98%), ADBI (purity 98%), and AHMI (purity 98%) were purchased from Spectra 2000 (Rome, IT). Deuterated internal standard AHTN-D3 was purchased from LGC Standards (Manchester, USA). A mix working solution was prepared using standards to obtain a final concentrations of 40 ng μL−1 for HHCB and HHCB-L and 8 ng μL−1 for ADBI, AHMI, and AHTN according to relationship between compounds previously evaluated for Italian wastewaters (Tasselli and Guzzella 2020; Tasselli et al. 2021). AHTN-D3 was diluted in acetone for water analyses and in n-hexane for sediment samples to a final concentration of 1 ng μL−1. Solutions were stored at − 30 °C in 25 mL flasks in the dark to prevent photolysis. Employed solvents were all obtained from VWR International with analytical grade (Radnor, PA, USA).
Water and sediment analysis
Water samples were analyzed using method already published in (Tasselli and Guzzella 2020), slightly modified for freshwater samples. Briefly, 0.5 L was filtered using glass fiber filters (nominal pore of 0.7 μm) before adding 25 ng of deuterated internal standard AHTN-D3. Samples were then reversed solid-phase extracted using C18 cartridges (Avantor™ BAKERBOND™, Radnor, PA, USA) and eluted with n-hexane:dichloromethane 1:3 v/v and n-hexane:dichloromethane 1:1 v/v. Samples were then concentrated at 30 °C using N2 flux (MultiVap 10, LabTech, Italy) to 0.5 mL and transferred in micro-vials for GC–MS/MS analysis.
Samples were also analyzed for pH, conductivity (potentiometric methods), alkalinity (Gran’s titration), reactive and total phosphorus, N–NH4, total nitrogen, reactive silica (UV–VIS spectrophotometry), N–NO3 (ion chromatography), total organic carbon (TOC) (high-temperature catalytic combustion) using standard methods for freshwater samples (APHA, AWWA, WEF, 2012; APAT, IRSA-CNR 2003). These analyses were not performed for the Margorabbia River.
Sediment samples of 0.4 g were extracted with n-hexane:acetone 3:1 v/v using a hot-Soxhlet apparatus (Büchi B-811 Flawil, Switzerland), concentrated using N2 flux at 0.1 mL, solvent-exchanged in 1 mL acetone, and resuspended in 200 mL of ultrapure water. Samples were then processed according to method descripted for water samples.
Details about analytical method in GC–MS/MS can be found in Tasselli and Guzzella (2020).
For each sediment sample, organic carbon content was determined in 0.2–0.5 g d.w. samples by back-titration after oxidation with potassium dichromate in the presence of sulfuric acid according to Walkley and Black (Schumacher 2002).
Quality assurance and quality control
Methods for PCM analyses in water and sediment samples were validated based on the procedure descried by EuraChem (Magnusson and Örnemark 2014). Linearity, limits of detection, precision, and accuracy were evaluated. Linearity was checked by injection of six standard samples containing all PCMs at different concentration levels. Limit of detection was calculated based on the signal-to-noise ratio (S/N) of individual peaks, assuming a ratio of 3:1. Since certified reference materials are not available for these chemicals, precision and accuracy were evaluated by spiking real water and sediment samples in triplicate at different concentration levels to cover the entire concentration range of the analyzed samples. Details about validation parameters can be found in Supplementary Materials (Tables S1 and S2). Due to the widespread use of PCMs, precautions were employed to prevent laboratory contamination. Laboratory personnel avoided the use of scented personal care products, and all glassware was acetone washed prior to using it. Procedural blanks were analyzed for every extraction batch of water and sediment and all samples were blank subtracted since trace levels of HHCB, HHCB-L, and AHTN were detected.
Preliminary environmental risk assessment of HHCB and AHTN
The environmental risk assessment of HHCB and AHTN in surface water and sediment was performed according to the hazard quotient (HQ) approach (Raybould et al. 2011). HQ represents the ratio of the potential exposure to a substance and the level at which no adverse effects are expected. In this work, the potential environmental risk posed by PCMs in freshwaters and sediments was assessed by calculating the HQ according to Eq. (1):
where MEC refers to the measured environmental concentration; and PNEC refers to the predicted no effect concentration. According to European Union Risk Assessments, PNEC values for HHCB and AHTN were 4400 ng L−1 and 2800 ng L−1 for water and 1970 ng g−1 d.w. and 1720 ng g−1 d.w. for sediment (European Commission 2008a, b). PNEC values were calculated from results of prolonged toxicity tests carried out on algae, the invertebrates Daphnia magna and Acartia tonsa, and fish for water and regarding midge larvae, amphipods, and worms regarding sediments. For both matrices, an assessment factor of 10 was applied for PNEC calculation. Overall, HQ values > 1.0 indicate that a high risk is expected, while values between 0.1 and 1.0 indicate a medium risk, and values < 0.1 indicate a low risk (Sánchez-Bayo et al. 2002). The combined ecological risk was also calculated by the sum of the individual HQi of each PCM (Guo et al. 2013).
Statistical analysis
Statistical analyses were carried out using R software (version 4.2.0) and Past (4.03). The Shapiro–Wilk test was used to verify the data normality while the homogeneity of variances was evaluated using the Levene test. In case of significance (p < 0.05), non-parametric statistical tests were employed. In particular, the Kruskal–Wallis rank sum test and Dunn’s post-hoc test were performed to compare fragrance concentrations in the various tributaries, while Spearman correlation analysis was employed to evaluate possible relationships between concentrations of different fragrances and river chemical parameters. Map of sampling sites was created using Google Earth version 7.3.6.9345.
Results and discussion
Concentrations and spatial distribution of PCMs in river systems
Water samples
Results concerning fragrance quantification in freshwater samples are here summarized. Ticino Tributary showed always total PCM concentrations < LOD while in River Toce only HHCB was detected at concentrations below 20 ng L−1. We hypothesized that the measured very low concentrations in these rivers were due to a combination of a lower contamination level of their waters with respect to the other tributaries and to their high water flow (Fig. S1) which can dilute PCMs to values below the limit of detection. Since these tributaries did not significantly contribute to the apport of synthetic fragrances at Lake Maggiore basin even during the summer period, characterized by seasonal tourism in those areas, their analysis was not continued in the following period.
Considering other tributaries, detected PCM concentrations vary significantly between rivers (Fig. 2). Boesio and Bardello rivers were the most polluted by PCMs, with mean concentrations of 504 ± 130 ng L−1 and 417 ± 137 ng L−1, respectively. These rivers, characterized by a modest discharge (Fig. S1), cross heavily anthropized areas. From the inlet to the mouth, these rivers receive numerous discharges of wastewaters, both of civil and industrial types which contribute to accentuate the poor quality of the waters (ARPA 2013; Mosello et al. 2001) and could represent punctual sources of synthetic fragrances release into the environment, as demonstrated in previous studies (Tasselli and Guzzella 2020; Tasselli et al. 2021). For example, the WWTP of the city of Casalzuigno with its effluent located along Boesio River or the WWTP of the city of Gavirate with its effluent located along the Bardello River (ARPA 2013).
Margorabbia River showed lower and less variable levels of synthetic musk, registering mean concentrations of 184 ± 74 ng L−1. A mean concentration of 68 ± 23 ng L−1 and 6 ± 4 ng L−1 of total fragrances was instead measured at Tresa and Ticino Emissary rivers. The low concentrations of fragrances detected in these rivers might be due to a dilution effect caused by the lakes from which they originate, Lake Ceresio and Lake Maggiore, respectively. Statistically significant differences were evidenced between Ticino Emissary and Bardello (p < 0.001), Boesio (p < 0.001), and Margorabbia (p < 0.05) rivers; on the other hand, no differences were found between Bardello, Boesio, and Margorabbia rivers, and between Tresa and Ticino Emissary rivers (p > 0.05). Regarding Margorabbia River, the presence of a municipal WWTP effluent along the river course, which may introduce synthetic fragrances, is documented as well (ARPA 2013) and could therefore explain the slightly higher concentrations measured in this river before its confluence with River Tresa, a few hundred meters before flowing into Lake Maggiore.
Figure 3 shows monthly values of the total fragrances in the water samples of the study rivers.
In general, a similar pattern was evidenced for all the rivers, with higher concentrations in June–July and December–January, even if the minimum concentration of fragrances was measured in all rivers during August, 2021. This result agrees with the hypothesis of dilution already demonstrated for wastewaters (Tasselli et al. 2021): in fact, sampling during August, 2021, was performed after 1 month of high river discharge (Fig. S1) as an effect of quite heavy rainfall in July, 2021, which may have diluted PCM concentrations. Maximum concentration was instead measured in July, 2021, at Margorabbia (331 ng L−1) before the rain events, in October and January, 2022, at Boesio (674 ng L−1 and 635 ng L−1), in December, 2021, at Bardello (629 ng L−1) and in January, 2022, at Tresa (106 ng L−1). Also by considering the entire annual pattern, Boesio and Bardello were confirmed as the most contaminated rivers by synthetic fragrances.
Sediments
Due to their hydrophobic nature, organic compounds are mainly adsorbed on sediment organic matter so that the normalization of data on organic carbon (OC) content leads to comparison between sites with different OC (Pisanello et al. 2016). For this reason, total OC was measured for each sediment samples and concentration of PCMs was normalized to this parameter. Figure 4 shows PCM concentration OC normalized in each sediment samples of Lake Maggiore tributaries analyzed during the three sampling campaigns.
In Ticino Tributary the analyzed compounds were always < LOD, while in Toce River 235 ng g−1 OC of total synthetic fragrances were detected only in April 2022. Concentrations < 25 ng g−1 OC were observed in Margorabbia and Tresa rivers both in July and October, 2021, while in April, 2022, total synthetic fragrances in Margorabbia River were below the detection limit. The minimum total PCM concentrations were found for almost all rivers in October, 2021. Considering the entire sampling year, the maximum concentration of total PCMs was measured in the sediments of Bardello River (329 ng g−1 OC) in July, 2021, which could be considered the most contaminated river by polycyclic musk fragrances. In this case, also the contribution probably deriving from the WWTP effluent of the city of Gavirate may have determined the high concentrations measured in the river. As for water samples, synthetic fragrances in sediments seemed to be influence by the respective river water flow. In fact, higher concentrations were measured in all tributaries during July, 2021, and April, 2022, after periods of scarce rain events and modest water flow especially for Toce and Bardello rivers (Fig. S1), while in October, 2021, the concentration of synthetic fragrances decreased in all rivers probably due to a dilution effect caused by the higher mean flow rate registered in all rivers during months prior to sampling.
Composition and origin of PCMs in rivers
In all the tributaries of Lake Maggiore ADBI and AHMI were always lower than the LOD value, except in few samples of Boesio and Bardello rivers, where ADBI was detected at trace levels with concentrations always below 1 ng L−1. HHCB was the main measured compound in all freshwater samples and always represented above the 70% of the total PCMs in the samples. In rivers located on the more anthropized shore, Tresa, Margorabbia, Boesio, and Bardello, a low contribution of HHCB-L was measured, with values in the range 12–26% while AHTN contributed only for < 5% of the total PCMs in the abovementioned rivers except Tresa. In Ticino Emissary, almost all fragrances detected in water samples were represented by HHCB. This type of profile regarding synthetic fragrances generally agrees with their use in commercial products in which HHCB and AHTN are the main utilized compounds (Clara et al. 2011). The low concentrations of AHTN respect to HHCB may also be explained by a photochemical degradation process: in fact, Sanchez-Prado et al. (2004) evaluated under laboratory conditions the degradation of six polycyclic musk under UV irradiation measuring high degradation rates for AHTN in respect to HHCB and even (Buerge et al. 2003) carried out various UV-irradiation experiments using lake water samples and measured half-lives of 4 h for AHTN and 135 h for HHCB. Regarding sediments, HHCB was the main detected polycyclic musk (Fig. 4), as expected from its chemical properties as hydrophobicity (KOW = 5.3), while the contribution of its metabolite HHCB-L was lower than in water samples, since this compound is characterized by a higher polarity. The relation between HHCB and AHTN was further analyzed in all surface water samples of this study and a coefficient of determination (R2) of 0.91 was obtained (Fig. 5). Spearman correlation test expressed high significance between the two chemicals (ρ = 0.95; p < 0.001), supporting the hypothesis of the same origin of these compounds. HHCB/AHTN ratios were then calculated for each sample and results were compared to those already measured by Tasselli et al. (2021) in wastewater samples: the Mann–Whitney U test resulted not significant (p = 0.35), thus revealing that synthetic fragrances measured in surface waters may derive from outflows of wastewater treatment plants as previously evidenced in other studies worldwide (Horii et al. 2007; Zhang et al. 2020; Heredia et al. 2023).
Relations between fragrance occurrence and chemical parameters
The hypothesis of WWTP effluents as possible sources of synthetic fragrances measured in surface waters was further assessed by considering other chemical variables measured in the same rivers and periods. Mean values of the main chemical parameters measured in Lake Maggiore main tributaries and emissary are shown in Table 1 while detailed monthly data are reported in Supplementary Materials (Fig. S2).
According to these data, Bardello and Boesio are characterized by markedly higher concentrations of solutes (higher conductivity and alkalinity) and P and N compounds than the other rivers. In particular, TP and organic nitrogen concentrations indicate a higher level of contamination of Bardello and Boesio by untreated effluents. Previous studies and monitoring programs have highlighted the scarce water quality of these two rivers, due to various sources of pollution along their water course, from untreated domestic and industrial sewage to WWTP discharge (Guzzella et al. 2008; Corno et al. 2023; CNR IRSA 2019). In addition, River Bardello is the outflow of the highly eutrophic Lake Varese (Dresdi et al. in press). From these data it can be deduced that fragrances are more present in rivers affected by higher nutrient organic matter content. Possible relationships between concentrations of polycyclic musks measured in water and the main chemical parameters were further investigated and details are summarized in Table 2.
Polycyclic musk fragrances are positively correlated with chemical parameters related to solute concentrations in river water (alkalinity and conductivity), which are markedly higher in Boesio and Bardello rivers than in the other tributaries (Table 1). PCMs are also positively related with TP and N compounds which are indicators of nutrient enrichment due to contamination by untreated wastewater. Many studies relate the occurrence of polycyclic musk fragrances with anthropogenic impacts as human density and activities. For example, in China and Korea synthetic fragrances were demonstrated to be released in the environment through WWTP effluents since high concentrations were detected in proximity of these areas (Lee et al. 2010; Hu et al. 2011). Synthetic musk fragrances were detected even in Indian (Vimalkumar et al. 2021) and in some European rivers (Bendz et al. 2005; Sumner et al. 2010; Villa et al. 2012; Lange et al. 2015; Homem et al. 2016) in which human activities and the presence of discharges were identified as the main emitting sources. Generally, as demonstrated even in our study, dilution is the main mechanism affecting PCM concentration in surface waters (Lee et al. 2010; Chase et al. 2012; Vimalkumar et al. 2021). The farther is the WWTP effluent point from the sampling station and/or the greater is the water flow rate of the river itself, the lower is the measured fragrance concentration.
Starting from significant correlations derived from Spearman analysis, in Fig. 6 the resulting linear regressions between polycyclic musk fragrance concentrations and main parameters strictly related to anthropogenic impacts are shown.
Comparisons with other studies
Observed concentrations of HHCB and AHTN in freshwater samples were compared with data available from other studies (Table 3).
Regarding water samples, Boesio and Bardello rivers were generally one order of magnitude more contaminated by HHCB respect to Chinese rivers as Suzhou Creek, Haie River, and Songhua River (Zhang et al. 2008; Hu et al. 2011; Lu et al. 2015), while Nakdong River in Korea resulted more contaminated by HHCB, reaching peaks of 13,920 ng L−1 (Lee et al. 2010). This might be due to a combination of both the river discharge and the usage of PCMs in that area. In fact, Chinese rivers considered in Zhang et al. (2008) are large rivers with a high water flow that is able to dilute PCM concentrations discharged by WWTP effluents, while Lee et al. (2010) evidenced how the predominance of HHCB in the analyzed samples was consistent with the high usage of HHCB in Korea, around the 80% of the total usage of PCMs in the Korean market. Considering the other tributaries of Lake Maggiore as Toce and Ticino Tributary, together with Ticino Emissary, our results were comparable, for example, to the low concentrations detected in the Hudson River located in the USA (Reiner and Kannan 2011). Again, this was probably due to the high mean water flow of Ticino and Toce Rivers, 350 m3 s−1 and 70 m3 s−1, respectively, comparable to the water flow of Hudson River, 606 m3 s−1. In this case, a strong dilution effect may be assumed. Regarding Europe, our results were almost in line with concentrations detected in other European Rivers (Moldovan 2006; Sumner et al. 2010; Lange et al. 2015). However, rivers located in densely populated area as Berlin (Fromme et al. 2001) and Molgora River in Italy (Villa et al. 2012) registered higher concentrations than our study probably because pollution caused by domestic discharges is here more intensive and the rivers are not able to dilute PCMs to reach lower concentration levels.
Considering sediments, concentrations from previously published studies are summarized in Table 4.
When compared to studies conducted worldwide, our results were similar to that reported for most regions and countries in terms of relative contribution of HHCB and AHTN. In fact, worldwide, HHCB is the main polycyclic musk detected in sediment samples. Regarding absolute concentrations, depending on the considered river, our result can be comparable to rivers with high water flows which can dilute fragrance concentration or to rivers which cross heavy anthropized areas that can impact on freshwater ecosystems by discharging these chemicals through WWTP effluents. For example, data of Ticino Tributary, Toce, Tresa, and Margorabbia rivers of this study are comparable to other emissaries and large rivers as Suzhou Creek River in China (Zhang et al. 2008), which is one of the emissaries of Lake Tai, and Songhua and Lippe Rivers, characterized by high water flow, 2470 m3 s−1 and 241 m3 s−1, respectively (Kronimus et al. 2004; Lu et al. 2015). On the contrary, concentrations of synthetic musks detected in Boesio and Bardello sediments are comparable to that measured in other anthropized rivers as Hudson River in the USA (Reiner and Kannan 2011), the North Canal River watershed in China, an urban catchment located in the megacity of Beijing (Zhang et al. 2020), and rivers crossing other urban catchments as Singapore and Berlin (Fromme et al. 2001; Wang and Kelly 2017). Besides synthetic musk fragrances, the presence of other persistent organic contaminants, including those of industrial origin, such as brominated and chlorinated compounds, is widely documented in these two rivers (Marziali et al. 2021), which therefore appear to be the main contamination sources of Lake Maggiore.
Potential environmental risk assessment of HHCB and AHTN
The environmental risk assessment of HHCB and AHTN in surface water and sediment was performed according to the Hazard Quotient (HQ) approach already employed in other studies (Raybould et al. 2011). Considering for water HHCB and AHTN PNEC values of 4400 ng L−1 and 2800 ng L−1, respectively (European Commission 2008b, a) our data showed HQ for AHTN always < 0.1 in all samples, thus indicating a low risk level for this compound. Similarly, HHCB expressed low risk (HQ < 0.1) in most of the analyzed samples even if sometimes exceeding the value of 0.1 in winter samples of Bardello and Boesio rivers and even in spring only in Boesio River, thus posing a medium risk for the environment. Therefore, exposure of aquatic organisms to polycyclic musk fragrances seems to be higher in winter, probably because of the lower river dilution factor that can mitigate discharge impact. Similarly, even in Indian (Vimalkumar et al. 2021) and Chinese (Zeng et al. 2018; Wang et al. 2018) rivers characterized by the presence of important discharges of domestic and industrial wastewaters, synthetic musks do not pose any health hazard to aquatic organisms. The combined ecological risk was calculated on the sum of HQs of HHCB and AHTN (Guo et al. 2013). A combined HQ ≤ 0.01, between 0.01 and 0.1, between 0.1 and 1, and ≥ 1.0 can be traduced into an ecological risk level of no, probable low, medium, and high risks (Lee et al. 2014). In water samples, combined HQ resulted in a probable low risk in Margorabbia, Bardello, and Boesio rivers, sometimes with peaks reaching the expected medium risk range in winter samples in Boesio and Bardello. No ecological risk resulted from combined HQ of Tresa and Ticino Emissary water samples.
Regarding sediments, in the present study measured concentrations of synthetic musks were lower than their respective PNEC values for sediment-dwelling organisms, and the resulting HQ of AHTN was below 0.1 in all rivers indicating that the risk caused by this chemical was low in all sampling sites. Regarding HHCB, HQ was again below 0.1 in all tributaries except in Boesio and Bardello rivers in which a medium risk regarding HHCB was evidenced. Considering again the combined ecological risk for sediments, Ticino Emissary showed a probable low risk level, while Boesio and Bardello rivers expressed an expected medium risk level, with a combined HQ between 0.1 and 1. Therefore, even for sediment-dwelling organisms, Boesio and Bardello rivers expressed a medium risk level. In general, since field-derived log KOC values for HHCB and AHTN are in the range of 3.86–4.86 (Fooken 2004; Wang et al. 2018), these compounds are expected to accumulate primarily in suspended particulates, sediments, and within organisms resulting in a biomagnification hazard as already evidenced in other aquatic ecosystems (Zhang et al. 2013; Hu et al. 2011).
However, this preliminary approach does not take into consideration other pollutants which may be present in surface waters or sediments, and which might contribute to increase in the combined ecological risk with synergistic and additive effects. In addition, HQ approach does not take into consideration processes such as bioaccumulation and contaminant metabolisms in aquatic organisms together with long-term exposure effects (Guo et al. 2013). Further data are necessary to evaluate potential adverse effects of polycyclic musk under the contest of long-term exposure and pollutant mixtures.
Conclusions
In this study, the presence of synthetic musk fragrances was investigated in surface waters and sediments of the main tributaries of a large deep subalpine lake in Northern Italy. A wide range of PCM concentration was measured in the considered rivers, from few ng L−1 to values above 500 ng L−1. HHCB was the main detected compound in all samples, as expected from its extensive usage in commercial products. Once released in surface waters, synthetic musks may be accumulated in sediments which therefore may represent their final sink. Levels of synthetic musks were strictly dependent on anthropogenic pressures along the river courses, particularly the presence of WWTP which can discharge these chemicals through their effluents.
Chemical data gathered during the study, in particular, N and P concentrations, confirmed this hypothesis since high PCM values were detected in the more polluted rivers located in areas where intensive anthropogenic pressures are present. The ecological risk posed by individual and combined PCMs in freshwaters and sediments are still acceptable; however, Boesio and Bardello rivers showed an expected medium risk levels during dry seasons, where dilution does not compensate the input of these chemicals from WWTPs. A site-specific ecological risk assessment together with bioaccumulation studies is needed to fully understand the potential impact of these chemicals on aquatic organisms.
This study provides the first valuable information regarding the presence of synthetic musks in the studied ecosystem. However, more data are needed to identify punctual emission sources of these compounds, alongside with bioaccumulation studies to evaluate the effective bioavailability and further investigate the ecological risk posed by synthetic musks in the environment.
Data availability
Not applicable.
References
Ambrosetti W, Barbanti L, Rolla A et al (2012) Hydraulic paths and estimation of the real residence time of the water in Lago Maggiore (N. Italy): application of massless markers transported in 3D motion fields. J Limnol 71:e2–e2. https://doi.org/10.4081/jlimnol.2012.e2
APAT, IRSA-CNR (2003) Metodi analitici per le acque (in Italian). APAT Manuali e Linee Guida, 29. Available online: https://www.irsa.cnr.it/wp/wpcontent/uploads/2022/04/Vol1_Sez_1000_Indice_ParteGenerale.pdf. Accessed 25 Sept 2023
APHA, AWWA, WEF (2012) Standard Methods for examination of water and wastewater, 22nd edn. American Public Health Association, Washington, p 1360
ARPA Lombardia (Agenzia Regionale per la Protezione dell’Ambiente) (2013) Stato delle acque superficiali della provincia di Varese. Rapporto annuale 2012, pp 74 (in Italian). Available online: https://www.arpalombardia.it/media/hudd5chq/stato-delle-acque-superficiali-della-provincia-di-varese_2012.pdf. Accessed 25 Sept 2023
Artola-Garicano E, Borkent I, Damen K et al (2003) Sorption kinetics and microbial biodegradation activity of hydrophobic chemicals in sewage sludge: model and measurements based on free concentrations. Environ Sci Technol 37:116–122. https://doi.org/10.1021/es020115y
Bendz D, Paxéus NA, Ginn TR, Loge FJ (2005) Occurrence and fate of pharmaceutically active compounds in the environment, a case study: Höje River in Sweden. J Hazard Mater 122:195–204. https://doi.org/10.1016/j.jhazmat.2005.03.012
Bester K (2004) Retention characteristics and balance assessment for two polycyclic musk fragrances (HHCB and AHTN) in a typical German sewage treatment plant. Chemosphere 57:863–870. https://doi.org/10.1016/j.chemosphere.2004.08.032
Bester K (2005) Polycyclic musks in the Ruhr catchment area—transport, discharges of waste water, and transformations of HHCB, AHTN and HHCB-lactone. J Environ Monit 7:43–51. https://doi.org/10.1039/B409213A
Brausch JM, Rand GM (2011) A review of personal care products in the aquatic environment: Environmental concentrations and toxicity. Chemosphere 82:1518–1532. https://doi.org/10.1016/j.chemosphere.2010.11.018
Buerge IJ, Buser H-R, Müller MD, Poiger T (2003) Behavior of the polycyclic musks HHCB and AHTN in lakes, two potential anthropogenic markers for domestic wastewater in surface waters. Environ Sci Technol 37:5636–5644. https://doi.org/10.1021/es0300721
Chase DA, Karnjanapiboonwong A, Fang Y et al (2012) Occurrence of synthetic musk fragrances in effluent and non-effluent impacted environments. Sci Total Environ 416:253–260. https://doi.org/10.1016/j.scitotenv.2011.11.067
Clara M, Gans O, Windhofer G et al (2011) Occurrence of polycyclic musks in wastewater and receiving water bodies and fate during wastewater treatment. Chemosphere 82:1116–1123. https://doi.org/10.1016/j.chemosphere.2010.11.041
CNR IRSA. Sede di Verbania (2019) Ricerche sull’evoluzione del Lago Maggiore. Aspetti limnologici. Programma triennale 2016–2018. Campagna 2018 e rapporto triennale 2016–18. Commissione Internazionale per la protezione delle acque italo-svizzere (ed.): pp 160 (in Italian). Available online: https://www.cipais.org/web/wp-content/uploads/2023/05/S1-RM-CIPAIS_Rapporto_2018_triennio_16-18_Limnologia_Maggiore.pdf. Accessed 25 Sept 2023
CNR IRSA. Sede di Verbania (2022) Ricerche sull’evoluzione del Lago Maggiore. Aspetti limnologici. Programma triennale 2019–2021. Campagna 2021 e Rapporto triennale 2019–2021. Commissione Internazionale per la protezione delle acque italo-svizzere (ed.): pp 190 (in Italian). Available online: https://www.cipais.org/web/wp-content/uploads/2023/04/S1-RM-CIPAIS_Rapporto_Limnologia_2021_finale_Maggiore.pdf. Accessed 25 Sept 2023
CNR IRSA. Sede di Verbania (2023) Ricerche sull’evoluzione del Lago Maggiore. Aspetti limnologici. Programma triennale 2022–2024. Campagna 2022. Commissione Internazionale per la protezione delle acque italo-svizzere (ed.): pp 112 (in Italian) (in press)
Corno G, Ghaly T, Sabatino R et al (2023) Class 1 integron and related antimicrobial resistance gene dynamics along a complex freshwater system affected by different anthropogenic pressures. Environ Pollut 316:120601. https://doi.org/10.1016/j.envpol.2022.120601
Dresdi C, Rogora M, Buzzi F, Beghi A, Magni D, Canziani A, Fenocchi A (in press) A modelling approach to evaluate the effectiveness of hypolimnetic withdrawal for the restoration of eutrophic Lake Varese (Northern Italy). J Environ Manag
European Commission (2008a) Risk Assessment Report on 1-(5,6,7,8-tetrahydro-3,5,5,6,8,8-hexamethyl-2-napthyl)ethan-1-one – AHTN (cas no: 1506–02–1). European Chemicals Bureau, Ispra, Italy (2008) final approved version, May 2008. Available online: https://echa.europa.eu/documents/10162/a0da6504-8d8c-49af-8614-fe396c556c43. Accessed 25 Sept 2023
European Commission (2008b) Risk Assessment Report on 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclo-penta-g-2-benzopyran – HHCB (cas no: 1222–05–5). European Chemicals Bureau, Ispra, Italy (2008) final approved version, May 2008. Available online: https://echa.europa.eu/documents/10162/947def3b-bbbf-473b-bc19-3bda7a8da910. Accessed 25 Sept 2023
Fooken C (2004) Synthetic musks in suspended particulate matter (SPM), sediment, and sewage sludge. In: Rimkus GG (ed) Series Anthropogenic Compounds. Springer, Berlin, Heidelberg
Fromme H, Otto T, Pilz K (2001) Polycyclic musk fragrances in different environmental compartments in Berlin (Germany). Water Res 35:121–128. https://doi.org/10.1016/S0043-1354(00)00233-5
Guilizzoni P, Levine SN, Manca M et al (2012) Ecological effects of multiple stressors on a deep lake (Lago Maggiore, Italy) integrating neo and palaeolimnological approaches. J Limnol 71:1. https://doi.org/10.4081/jlimnol.2012.e1
Guo G-H, Wu F-C, He H-P et al (2013) Screening level ecological risk assessment for synthetic musks in surface water of Lake Taihu, China. Stoch Environ Res Risk Assess 27:111–119. https://doi.org/10.1007/s00477-012-0581-1
Guzzella L, Roscioli C, Binelli A (2008) Contamination by polybrominated diphenyl ethers of sediments from the Lake Maggiore basin (Italy and Switzerland). Chemosphere 73:1684–1691. https://doi.org/10.1016/j.chemosphere.2008.06.073
HERA (Human and Environmental Risk Assessment on ingredients of household cleaning products) (2008) Polycyclic musks AHTN (CAS 1506-02-1) and HHCB (CAS 1222-05-05). http://www.heraproject.com/files/28-E-36551E10-F8EFE807-E4199B9BB0076A9F.pdf
Heredia AC, Cabrera-Peralta J, Ocaña-Rios I, Peña-Alvarez A (2023) Simple and rapid preparation of homemade SPME PDMS fibers and their application to the analysis of personal care products in water samples. Chem Pap 77:2039–2049. https://doi.org/10.1007/s11696-022-02608-z
Herrera López S, Hernando MD, Gómez MJ et al (2013) Investigation of Galaxolide degradation products generated under oxidative and irradiation processes by liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry and comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 27:1237–1250. https://doi.org/10.1002/rcm.6575
Homem V, Alves A, Alves A, Santos L (2016) Ultrasound-assisted dispersive liquid–liquid microextraction for the determination of synthetic musk fragrances in aqueous matrices by gas chromatography–mass spectrometry. Talanta 148:84–93. https://doi.org/10.1016/j.talanta.2015.10.049
Hong J-H, Lee J-Y, Ha H-J et al (2021) Occurrence and sources of synthetic musk fragrances in the sewage treatment plants and the Han River. Korea Water 13:392. https://doi.org/10.3390/w13040392
Horii Y, Reiner JL, Loganathan BG et al (2007) Occurrence and fate of polycyclic musks in wastewater treatment plants in Kentucky and Georgia, USA. Chemosphere 68:2011–2020. https://doi.org/10.1016/j.chemosphere.2007.04.054
Hu Z, Shi Y, Cai Y (2011) Reprint of: Concentrations, distribution, and bioaccumulation of synthetic musks in the Haihe River of China. Chemosphere 85:262–267. https://doi.org/10.1016/j.chemosphere.2011.09.002
Hua Y, Wang L, Zheng M et al (2022) Occurrence of synthetic musks in the sediment-seawater system of the Yellow Sea and East China Sea during wet and dry seasons. Sci Total Environ 833:155164. https://doi.org/10.1016/j.scitotenv.2022.155164
Huang W, Xie Z, Yan W et al (2016) Occurrence and distribution of synthetic musks and organic UV filters from riverine and coastal sediments in the Pearl River estuary of China. Mar Pollut Bull 111:153–159. https://doi.org/10.1016/j.marpolbul.2016.07.018
Kannan K, Reiner JL, Yun SH et al (2005) Polycyclic musk compounds in higher trophic level aquatic organisms and humans from the United States. Chemosphere 61:693–700. https://doi.org/10.1016/j.chemosphere.2005.03.041
Kronimus A, Schwarzbauer J, Dsikowitzky L et al (2004) Anthropogenic organic contaminants in sediments of the Lippe river, Germany. Water Res 38:3473–3484. https://doi.org/10.1016/j.watres.2004.04.054
Lange C, Kuch B, Metzger JW (2015) Occurrence and fate of synthetic musk fragrances in a small German river. J Hazard Mater 282:34–40. https://doi.org/10.1016/j.jhazmat.2014.06.027
Lee I-S, Lee S-H, Oh J-E (2010) Occurrence and fate of synthetic musk compounds in water environment. Water Res 44:214–222. https://doi.org/10.1016/j.watres.2009.08.049
Lee I-S, Kim U-J, Oh J-E et al (2014) Comprehensive monitoring of synthetic musk compounds from freshwater to coastal environments in Korea: with consideration of ecological concerns and bioaccumulation. Sci Total Environ 470–471:1502–1508. https://doi.org/10.1016/j.scitotenv.2013.07.070
Lou Y, Wang J, Wang L et al (2016) Determination of synthetic musks in sediments of Yellow River Delta Wetland, China. Bull Environ Contam Toxicol 97:78–83. https://doi.org/10.1007/s00128-016-1814-7
Lu B, Feng Y, Gao P et al (2015) Distribution and fate of synthetic musks in the Songhua River, Northeastern China: influence of environmental variables. Environ Sci Pollut Res 22:9090–9099. https://doi.org/10.1007/s11356-014-3973-6
Magnusson B, Örnemark U (2014) The fitness for purpose of analytical methods: a laboratory guide to method validation and related topics, 2nd edn. Eurachem guide, p 57
Marziali L, Guzzella L, Salerno F et al (2021) Twenty-year sediment contamination trends in some tributaries of Lake Maggiore (Northern Italy): relation with anthropogenic factors. Environ Sci Pollut Res 28:38193–38208. https://doi.org/10.1007/s11356-021-13388-6
Moldovan Z (2006) Occurrences of pharmaceutical and personal care products as micropollutants in rivers from Romania. Chemosphere 64:1808–1817. https://doi.org/10.1016/j.chemosphere.2006.02.003
Mosello R, Barbieri A, Brizzio MC et al (2001) Nitrogen budget of Lago Maggiore: the relative importance of atmospheric deposition and catchment sources. J Limnol 60:27–40. https://doi.org/10.4081/jlimnol.2001.27
OSPAR (2002) CEMP Guidelines for monitoring contaminants in sediments. (OSPAR Agreement 2002-16). OSPAR Commission, Monitoring guidelines, ref. no. 2002-16. Revised in 2018. Available online: https://www.ospar.org/work-areas/cross-cutting-issues/cemp. Accessed 25 Sept 2023
Peck AM, Linebaugh EK, Hornbuckle KC (2006) Synthetic musk fragrances in Lake Erie and Lake Ontario Sediment Cores. Environ Sci Technol 40:5629–5635. https://doi.org/10.1021/es060134y
Peng F-J, Pan C-G, Zhang M et al (2017) Occurrence and ecological risk assessment of emerging organic chemicals in urban rivers: Guangzhou as a case study in China. Sci Total Environ 589:46–55. https://doi.org/10.1016/j.scitotenv.2017.02.200
Pisanello F, Marziali L, Rosignoli F et al (2016) In situ bioavailability of DDT and Hg in sediments of the Toce River (Lake Maggiore basin, Northern Italy): accumulation in benthic invertebrates and passive samplers. Environ Sci Pollut Res 23:10542–10555. https://doi.org/10.1007/s11356-015-5900-x
Ramos S, Homem V, Alves A, Santos L (2015) Advances in analytical methods and occurrence of organic UV-filters in the environment — a review. Sci Total Environ 526:278–311. https://doi.org/10.1016/j.scitotenv.2015.04.055
Raybould A, Caron-Lormier G, Bohan DA (2011) Derivation and interpretation of hazard quotients to assess ecological risks from the cultivation of insect-resistant transgenic crops. J Agric Food Chem 59:5877–5885. https://doi.org/10.1021/jf1042079
Reiner JL, Kannan K (2011) Polycyclic musks in water, sediment, and fishes from the Upper Hudson River, New York, USA. Water Air Soil Pollut 214:335–342. https://doi.org/10.1007/s11270-010-0427-8
Saidi H, Ciampittiello M, Dresti C, Ghiglieri G (2013) The climatic characteristics of extreme precipitations for short-term intervals in the watershed of Lake Maggiore. Theor Appl Climatol 113:1–15. https://doi.org/10.1007/s00704-012-0768-x
Sánchez-Bayo F, Baskaran S, Kennedy IR (2002) Ecological relative risk (EcoRR): another approach for risk assessment of pesticides in agriculture. Agr Ecosyst Environ 91:37–57. https://doi.org/10.1016/S0167-8809(01)00258-4
Sanchez-Prado L, Lourido M, Lores M et al (2004) Study of the photoinduced degradation of polycyclic musk compounds by solid-phase microextraction and gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom 18:1186–1192. https://doi.org/10.1002/rcm.1459
Sang W, Zhang Y, Zhou X et al (2012) Occurrence and distribution of synthetic musks in surface sediments of Liangtan River, West China. Environ Eng Sci 29:19–25. https://doi.org/10.1089/ees.2010.0241
Schumacher BA (2002) Methods for the determination of total organic carbon (toc) in soils and sediments. US Environmental Protection Agency, Office of Research and Development, Ecological Risk Assessment Support Center, Washington, DC, pp 1–23. Available online: https://d1wqtxts1xzle7.cloudfront.net/48609604/bs116.pdf?1473157675=&response-content-disposition=inline%3B+filename%3DMETHODS_FOR_THE_DETERMINATION_OF_TOTAL_O.pdf&Expires=1695636550&Signature=ELVU4urk9FsX46LqbN5OhsLyMKgJKb3wv4QWgdXdw68~ksf2KnQAhLBFWlANqhW8cGxoKOMfrwz2FLZ86X6rkl6GNmw6Garpn6dUvAlGVTKLZmbH5GILKYoxKg6v8gFgfHpa-VZNEnGfNG60LKLfPcfPofk21WOdGY-IsqVz1DUFVAPkzPyfNRbElkhImf3SofLJWMzylo~pXTKWPCh8KUtE05iQeP4GlKyJ3CPDtXDV8JnYh2Iq9lCR7v5s6M9xSqFDJscHapPoZ7ISInPfNZCywkIaGUeE~QQo4q2VS8Zh3DbRbxXA1uz2MuAZ7cRrjT41D7vbypGYsWYJxZxs6A__&Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA. Accessed 25 Sept 2023
Subedi B, Yun S, Jayaraman S et al (2014) Retrospective monitoring of persistent organic pollutants, including PCBs, PBDEs, and polycyclic musks in blue mussels (Mytilus edulis) and sediments from New Bedford Harbor, Massachusetts, USA: 1991–2005. Environ Monit Assess 186:5273–5284. https://doi.org/10.1007/s10661-014-3776-8
Sumner NR, Guitart C, Fuentes G, Readman JW (2010) Inputs and distributions of synthetic musk fragrances in an estuarine and coastal environment; a case study. Environ Pollut 158:215–222. https://doi.org/10.1016/j.envpol.2009.07.018
Sun P, Casteel K, Dai H et al (2014) Distributions of polycyclic musk fragrance in wastewater treatment plant (WWTP) effluents and sludges in the United States. Sci Total Environ 493:1073–1078. https://doi.org/10.1016/j.scitotenv.2014.04.038
Tasselli S, Guzzella L (2020) Polycyclic musk fragrances (PMFs) in wastewater and activated sludge: analytical protocol and application to a real case study. Environ Sci Pollut Res 27:30977–30986. https://doi.org/10.1007/s11356-019-06767-7
Tasselli S, Valenti E, Guzzella L (2021) Polycyclic musk fragrance (PMF) removal, adsorption and biodegradation in a conventional activated sludge wastewater treatment plant in Northern Italy. Environ Sci Pollut Res 28:38054–38064. https://doi.org/10.1007/s11356-021-13433-4
Ternes TA, Joss A, Siegrist H (2004) Scrutinizing pharmaceuticals and personal care products in wastewater treatment. Environ Sci Technol 38:392A-399A
Tumová J, Šauer P, Golovko O et al (2019) Effect of polycyclic musk compounds on aquatic organisms: a critical literature review supplemented by own data. Sci Total Environ 651:2235–2246. https://doi.org/10.1016/j.scitotenv.2018.10.028
US EPA (2001) Methods for collection, storage and manipulation of sediments for chemical and toxicological analyses: technical manual. EPA 823-B-01-002. U.S. Environmental Protection Agency, Office of Water, Washington, DC. Available online: https://www.epa.gov/sites/default/files/2015-09/documents/collectionmanual.pdf. Accessed 25 Sept 2023
Upadhyay N, Sun Q, Allen JO et al (2011) Synthetic musk emissions from wastewater aeration basins. Water Res 45:1071–1078. https://doi.org/10.1016/j.watres.2010.10.024
Villa S, Assi L, Ippolito A et al (2012) First evidences of the occurrence of polycyclic synthetic musk fragrances in surface water systems in Italy: spatial and temporal trends in the Molgora River (Lombardia Region, Northern Italy). Sci Total Environ 416:137–141. https://doi.org/10.1016/j.scitotenv.2011.11.027
Villa S, Di Nica V, Castiglioni S, Finizio A (2020) Environmental risk classification of emerging contaminants in an alpine stream influenced by seasonal tourism. Ecol Ind 115:106428. https://doi.org/10.1016/j.ecolind.2020.106428
Vimalkumar K, Nikhil NP, Arun E et al (2021) Synthetic musks in surface water and fish from the rivers in India: seasonal distribution and toxicological risk assessment. J Hazard Mater 414:125558. https://doi.org/10.1016/j.jhazmat.2021.125558
Wang Q, Kelly BC (2017) Occurrence and distribution of synthetic musks, triclosan and methyl triclosan in a tropical urban catchment: influence of land-use proximity, rainfall and physicochemical properties. Sci Total Environ 574:1439–1447. https://doi.org/10.1016/j.scitotenv.2016.08.091
Wang X-T, Hu B-P, Cheng H-X et al (2018) Spatial variations, source apportionment and potential ecological risks of polycyclic aromatic hydrocarbons and synthetic musks in river sediments in Shanghai, China. Chemosphere 193:108–117. https://doi.org/10.1016/j.chemosphere.2017.10.145
Weinberg I, Dreyer A, Ebinghaus R (2011) Waste water treatment plants as sources of polyfluorinated compounds, polybrominated diphenyl ethers and musk fragrances to ambient air. Environ Pollut 159:125–132. https://doi.org/10.1016/j.envpol.2010.09.023
Zeng X, Xu L, Liu J et al (2018) Occurrence and distribution of organophosphorus flame retardants/plasticizers and synthetic musks in sediments from source water in the Pearl River Delta, China. Environ Toxicol Chem 37:975–982. https://doi.org/10.1002/etc.4040
Zhang X, Yao Y, Zeng X et al (2008) Synthetic musks in the aquatic environment and personal care products in Shanghai, China. Chemosphere 72:1553–1558. https://doi.org/10.1016/j.chemosphere.2008.04.039
Zhang X, Xu Q, Man S et al (2013) Tissue concentrations, bioaccumulation, and biomagnification of synthetic musks in freshwater fish from Taihu Lake, China. Environ Sci Pollut Res 20:311–322. https://doi.org/10.1007/s11356-012-1095-6
Zhang H, Bu Q, Wu D, Yu G (2020) Polycyclic musks in surface water and sediments from an urban catchment in the megacity Beijing, China. Environ Pollut 263:114548. https://doi.org/10.1016/j.envpol.2020.114548
Acknowledgements
The authors thank Dr. Beatrice Bastante for her help in sampling and synthetic musk analyses.
Funding
Open access funding provided by IRSA - BRUGHERIO within the CRUI-CARE Agreement. The work was partially funded by the International Commission for the Protection of Italian-Swiss Waters (CIPAIS), Research Programs 2019–2021, 2022–2024 www.cipais.org (accessed on 18/05/2023).
Author information
Authors and Affiliations
Contributions
S.T.: project conceptualization, investigation, data curation, formal analysis, and writing–original draft preparation. M.R.: funding acquisition and writing–review and editing. A.O.: investigation. L.G.: funding acquisition and writing–review and editing.
Corresponding author
Ethics declarations
Ethical approval
Not applicable.
Consent to participate
Not applicable (this study does not contain any individual person’s data in any form).
Consent for publication
The authors declare that they agree with the publication of this paper in this journal.
Competing interests
The authors declare no competing interests.
Additional information
Responsible Editor: Ester Heath
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Tasselli, S., Rogora, M., Orrù, A. et al. Behaviour of synthetic musk fragrances in freshwaters: occurrence, relations with environmental parameters, and preliminary risk assessment. Environ Sci Pollut Res 30, 109643–109658 (2023). https://doi.org/10.1007/s11356-023-30030-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-023-30030-9