Abstract
The removal of n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals from wastewater using three dried seaweeds Ulva intestinalis Linnaeus (green alga), Sargassum latifolium (Turner) C.Agardh (brown alga), and Corallina officinalis Kützing (red alga) has been shown to evaluate their potential usage as inexpensive adsorbents. Under natural environmental conditions, numerous analytical methods, including zeta potential, energy dispersive X-ray spectroscopy (EDX), SEM, and FT-IR, are used in this study. The results showed that n-alkanes and polycyclic aromatic hydrocarbons adsorption increased with increasing contact time for all three selected algae, with a large removal observed after 15 days, while the optimal contact time for heavy metal removal was 3 h. S. latifolium dry biomass had more potential as bioadsorbent, followed by C. officinalis and then U. intestinalis. S. latifolium attained removal percentages of 65.14%, 72.50%, and 78.92% for light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons (PAHs), respectively, after 15 days. Furthermore, it achieved removal percentages of 94.14, 92.62, 89.54, 87.54, 82.76, 80.95, 77.78, 73.02, and 71.62% for Mg, Zn, Cu, Fe, Cr, Pb, Cd, Mn, and Ni, respectively, after 3 h. Carboxyl and hydroxyl from FTIR analysis took part in wastewater treatment. The zeta potentials revealed that algal cells have a negatively charged surface, and the cell surface of S. latifolium has a more negative surface charge than U. intestinalis and C. officinalis. Our study suggests that seaweeds could play an important role in wastewater treatment and thus help as an economical, effective, and ecofriendly bioremediation system for ecological health and life protection.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Water is extremely important; thus, there is a constant desire for its quality to be improved and preserved. With the increase of the industrial world and the global population, environmental issues have also gotten worse. Water pollution is a typical form of pollution that the globe is now dealing with. Overexploitation and deterioration of natural water supplies are the results of the rise in global population over the past century. (UNESCO 2021; Blanco-Vieites et al. 2022). Industrial activities are responsible for 23% of total water consumption (Blanco-Vieites et al. 2022). Our water resources’ quality is getting worse every day as a result of the persistent introduction of harmful substances into them. (Huang et al. 2021). Industrialization, civilization, agriculture, and other environmental and global changes are the primary causes of water contamination. (Haiba 2019; Ahmad et al. 2021). Even still, 80% of the wastewater that has not been cleaned is discharged into freshwater bodies used for domestic reasons. This gives rise to global water stress because of the increasing scarcity of freshwater resources. According to a study, by the year 2025, around 60% of the world’s population will experience water stress. (Khalid et al. 2018). Heavy metals, polycyclic aromatic hydrocarbons (PAHs), and a wide variety of n-alkanes can be found in wastewater particularly that is discharged by different types of industries (Haiba 2019; Redha 2020).
Water resources have been discovered to be contaminated by a few hundred organic contaminants such as alkanes and polycyclic aromatic hydrocarbons. Due to their numerous negative impacts and cancer-causing nature, these contaminants are extremely harmful. (Xia et al. 2022). Due to their noxiousness and abundance in the environment, the Environmental Protection Agency (EPA) has named 16 unsubstituted PAHs as the most aggressive pollutants (Qari and Hassan 2017). Urban areas are filled with PAHs, which are widely dispersed throughout the environment, either spontaneously (as components of crude oil) or due to man activity (like petroleum industries). Due to their cytotoxic characteristics, PAHs are particularly concerning (mutagenesis and carcinogenesis) (Haiba et al. 2019; Tomar and Jajoo 2021) and they can easily enter the food chain; this, in turn, negatively affects human health (Kottuparambil and Agusti 2020; Premnath et al. 2021).
Heavy metals are a type of water contaminants that pose a great threat to the ecological system (Redha 2020; Tang et al. 2023). Basically, the danger of heavy metals to all human life aspects lies in their non-degradable property and a very long biological half-life; thus, their bioaccumulation throughout the food chain causes significant consequences on higher trophic levels. (Zhang et al. 2019; Nathana et al. 2022). In addition, the toxicity of heavy metals also occurs in case of relatively low concentrations (Rehman et al. 2018). It has been found that exposure to toxic quantities of these substances significantly contributes to the deterioration of defense mechanisms and raises the chance of developing cancer (Yuan et al. 2019). Additionally, heavy metals make organic pollutants less biodegradable, prolonging their half-life in the environment and intensifying other toxic wastes’ impacts (Briffa et al. 2020).
Given the seriousness of these pollutants (n-alkanes, PAHs, and heavy metals), an immediate environmental issue that needs to be resolved is the efficient removal of them from wastewater. Traditional methods for removing these pollutants are mainly divided into chemical and physical methods. Despite the high cost, these techniques offer their benefits, requiring high amounts of energy, and can easily produce secondary pollution problems due to the formation of toxic by-products. Moreover, these techniques can be ineffectual when applied to low quantities of heavy metals because their effectiveness relies on concentration. (Shrestha et al. 2021; Zeng et al. 2022). Alternative methods to traditional ones for removing heavy metals from wastewater include biosorption. (Blanco-Vieites et al. 2022). Biosorption technology uses biological materials as adsorbents to clear the water bodies from a wide range of contaminants like aliphatic and aromatic hydrocarbons, pesticides, dyes, and heavy metal contaminates (Esfandiar et al. 2021; Dubey et al. 2023). Biosorption technology is simple, effective, more sustainable, ecofriendly, and low-cost. It has a promising future for development and is consistent with the countries’ present environmental protection philosophy. It is also a popular subject in recent studies (Cheng et al. 2019; Kumar et al. 2019). Bacteria, fungi, yeast, algae, and other microorganisms are the main types of materials that can be employed as biosorbents for bioremediation and the accumulation of organic pollutants and heavy metals (Chen et al. 2020). Because of its porous structure, substantial surface area, widespread dispersion, and other valuable aspects, algae have a promising removal action on various pollutants in water. Algae can be used as a straightforward and inexpensive sorbent for various contaminants from contaminated water (Rocha et al. 2020; Banerjee 2021; Chu et al. 2022). The comparative cost analysis showed that algae-based treatment is 10 times less expensive than traditional methods of treating industrial wastewater containing heavy metals. In addition, valuable metal ions like gold and silver can be recovered using algae (Jaafari and Yaghmaeian 2019).
Egypt’s industrial and population growth is swift and turbulent (Maghraby and Hassan 2021). The wastewater from Amia drain at the El-Tabia region in Alexandria, Egypt, consists of irrigation drainage and wastewater from the different industries, where this region hosts over 25 industrial activities. The present study explored the performance of Ulva intestinalis L. (green alga), Sargassum latifolium (Turner) C.Agardh (brown alga), and Corallina officinalis Kützing (red alga), in the removal of n-akanes, polycyclic aromatic hydrocarbons, and heavy metals from Amia drain water under natural climatic conditions. The work intends to create a naturally occurring, economically advantageous, and technically workable method for cleansing contaminated water resources. And it is worth saying that for the first time, the utilization of dried seaweeds as bioadsorbents for n-alkanes, polycyclic aromatic hydrocarbons, and heavy metal removal from wastewater under natural environmental conditions is done. Furthermore, this is the first work to study the removal of n-alkanes as individual hydrocarbons using dry seaweeds and study this large number of PAHs, in addition to the use of FTIR, SEM, EDX, and Zeta potential analyses to explain the ability of seaweeds to remove n-alkanes and PAHs.
Materials and methods
Algal species and sampling
Ulva intestinalis Linnaeus (green alga) and Corallina officinalis Kützing (red alga) were collected from Abu Qir in Alexandria, while Sargassum latifolium (Turner) C.Agardh (brown alga) was collected from the Gulf of Suez, Egypt coast; all samples were identified according to the methods of Aleem (1978), Aleem (1991), and Lipkin and Silva (2002) and be sure using the Algae Base website (Guiry 2020). Following collection, whole algae were thoroughly rinsed with seawater multiple times to remove any sand that may have become attached. Rhizoidal sections were also removed to prevent microbial infection. Algal materials were brought to the lab in plastic bags that contained saltwater. They are open-air dried for 24 h and oven dried for 3 h at 60 °C. Following fine grinding of the dried samples, they were stored in plastic bags at room temperature.
Wastewater sampling
Wastewater samples for culturing were collected from El Amia drain at El-Tabia pumping stations (Fig. 1) and discharged into Abu Qir Bay in Alexandria. Water samples were collected in empty n-hexane or dichloromethane glass bottles (not previously used for other purposes), and their composition is shown in Tables 1, 2, 3, and 4. These samples were taken to the laboratory without any filtering and kept in a cool, dark environment to preserve their properties.
Adsorption experiments
Experiments were conducted in 1-l beakers by mixing 3 g of dry algal mass with 1 l of wastewater. Beakers had earlier been rinsed with HNO3. The mixtures were periodically stirred for the necessary amount of time with aeration. Ten ml water were removed from the beakers after 1, 2, 3, 4, and 5 h then filtered by filter paper, and the residues (algal materials) are returned to the beakers to complete the experiment, while the filtrate metal content was determined using atomic absorption spectrophotometer measurements. Sampling was done after 5, 10, and 15 days to determine the ultimate concentration of hydrocarbons in filtrates (Wrabel and Peckol 2000; Akl and Ahmed 2022). The temperature was 25 °C and all studies were conducted in a natural climate.
Heavy metal and hydrocarbon removal rate (Re%) were determined as follows based on the beginning and end concentrations of each compound in the effluent (Tsekova et al. 2010):
where C0 and Cx are the concentrations of initial and final given pollutant in wastewater (mg/l−1), respectively. All trials were done in triplicates with the mean and standard error calculation.
Analytical procedures for wastewater
Analysis of the tested n-alkanes and polycyclic aromatic hydrocarbons (PAHs) in wastewater was performed according to Alkio et al. (2005). The extraction in a Teflon stopcock-supplied separating funnel was done for a duplicate measured volume of wastewater samples (1 l each) by 5 min shaking with 30 ml dichloromethane. Extraction was repeated three times. After the complete separation between the aqueous and organic phases, over the collection flask, the organic phase was passed through granular anhydrous sodium sulfate placed in filter paper. The organic extracts were combined in a 100-ml Erlenmeyer flask. Using a rotary evaporator, the extract was concentrated to a volume of around 5 ml, where the water bath temperature was maintained at around 40 °C, then to the final evaporation (for drying) by using a gentle stream of clean and dry nitrogen.
The extracted residue was re-solved in n-hexane in a GC vial fitted with a screw cap lined with Teflon; the final volume of extract was adjusted at 0.5 ml to inject GC–MS spectrometer (Thermo Scientific ISQ 2009) and helium at 1 ml/min as the carrier gas. The use was made of the TG-1MS column, composed of 100% dimethyl polysiloxane, fused silica, 30 m, 0.32-mm item diameter (i.d.), and 0.25-m film thickness. After 3 min of splitless injection mode, the injection port temperature reached 250 °C. Next, split mode with a split ratio of 1:100 was used. The temperature of the column was programmed to rise from 80 to 240 °F at a rate of 7 °C/min, then to 300 °F at a rate of 3 °C/min, and to maintain that temperature for 5 min (Alkio et al. 2005).
FTIR
FTIR was carried out through KBr pellets as the test substance at 4 cm−1 resolution and 400–4000 cm−1 range to demonstrate all potential functional groups on the algal biomass surface. The wave number (cm−1) on the Y axis and transmittance (%) on the X axis were used to plot the generated peaks.
SEM and EDX
A scanning electron microscope was applied to inspect the surface of the algal biomass both before and after the adsorption process (JSM 6490 LV, JEOL, Ltd., Tokyo, Japan). After samples had dried naturally on a double-sided carbon tape, they were photographed using various magnifications. Magnifications ranging from 15,000 to 35,000 nm and scanning voltages of 20 to 30 kV were used to measure the sizes. Using the same device, an energy dispersive X-ray (EDX) analysis of the component content and elemental distribution in algal biomasses between 0 and 12 keV was performed (Rajkumar et al. 2020).
Zeta potential (ζ)
The algal biomass effective surface charges at various variables were evaluated using a zeta analyzer (ZetaPlus, Brookhaven Instruments, Holtsville, NY, USA) at a range from − 200 to + 200 mV. A total of 25 g of algal biomass sample water dilution was done ten times. The sample was then run at 20 Hz for 15 min in a sonicator. Algal biomass was diluted to stop it from aggregating. (Basant et al. 2021).
Results and discussion
Assessment of the efficiencies of Ulva intestinalis L. dry biomass for the removal of light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons (PAHs)
Adsorption of light n-alkanes, heavy n-alkanes, and PAHs by U. intestinalis dry biomass at different time intervals was shown in Tables 2, 3, and 4. Hydrocarbon adsorption by this alga increased with the increase of contact time since a large decrease was observed after 15 days, where the concentration of light n-alkanes, heavy n-alkanes, and PAHs decreased from 51.12, 74.7, and 21.16 µg/l in initial concentration samples to 25.03, 36.07, and 8.23 µg/l, respectively, which represent half their values compared to the initial concentration. The alga achieved removal percentages 51.03%, 51.71%, and 61.10% for light n-alkanes, heavy n-alkanes, and PAHs, respectively, after 15 days (Fig. 2). This agrees with El-Shoubaky and Mohammad (2016) who reported that gasoline bioaccumulation differed significantly using the two algal species Ulva lactuca and Enteromorpha clathrate.
The supply and quality of water are thought to be seriously impacted by the rise in global pollution, industrialization, and rapid economic growth. Industrial, agricultural, and municipal wastewater are the three main sources of this waste, and they all contain pollutants such as xenobiotics, microplastics, regular alkanes, polycyclic aromatic hydrocarbons, and heavy metals (Ahmad et al. 2021; Bhatt et al. 2022; Ofrydopoulou et al. 2022).
The alternative biological agent abundantly found in nature algae is highlighted in this study as a potential sink for removing such hazardous compounds from water (Agüera et al. 2020; Ahmed et al. 2022; Sánchez et al. 2022; Satya et al. 2023). In order to clean and cleanse water, less dangerous chemicals can be used if algae are used. Algal biomass has also removed heavy metals and hydrocarbons in wastewater treatment facilities. The algae either store, absorb, or metabolize these harmful substances into significant levels (Ahmad et al. 2022; Alazaiza et al. 2022; Ghodrati et al. 2022).
Assessment of the efficiencies of Sargassum latifolium (Turner) C.Agardh dry biomass for removal of light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons (PAHs)
In the same trend, S. latifolium dry biomass showed a sharp decline in hydrocarbons concentration until the 15 days, where light n-alkanes decreased from 51.12 to 17.82 µg/l. Heavy n-alkanes decreased from 74.7 to 20.54 µg/l, and PAHs decreased from 21.16 to 4.46 µg/l (Tables 5, 6, and 7). The alga achieved removal percentages of 65.14%, 72.50%, and 78.92% for light n-alkanes, heavy n-alkanes, and PAHs, respectively, after 15 days (Fig. 2). Wrabel and Peckol (2000) reported that the analysis of treatment samples of oil spills showed a complete loss of n-alkanes after 18 days focusing the brown alga Fucus vesiclosus.
Assessment of the efficiencies of Corallina officinalis Kützing dry biomass for the removal of light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons (PAHs)
Tables 8, 9, and 10 show that the concentration of the remained light n-alkanes in wastewater using C. officinalis dry biomass decreased from 51.12 to 20.65 µg/l. Heavy n-alkanes remained in wastewater and decreased from 74.7 to 30.69 µg/l after 15 days. PAHs decreased from 21.16 to 6.16 µg/l after 15 days. The alga achieved removal percentages of 59.60%, 58.92%, and 70.88% for light n-alkanes, heavy n-alkanes, and PAHs, respectively, after 15 days (Fig. 2).
It is clear from the results that for all three selected algae, hydrocarbon adsorption increased with the increase of contact time since a large decrease was observed after 15 days. The observed decrease of light n-alkanes, heavy n-alkanes, and PAHs in the wastewater by the three studied species is largely due to the adsorption of these compounds to the algal surface. This suggestion was supported by some previous studies, which documented that most hydrophobic organic compounds (HOCs) tend to bioconcentrate (adsorbed) on algae (Saxena et al. 2021; Du et al. 2022).
Sargassum latifolium dry biomass was more potential as adsorbent for light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons from wastewater than U. intestinalis and C. officinalis. Our results are going in harmony with those of Chung et al. (2007), who reported that the removal percentage of aqueous phenanthrene by Sargassum was in the range of 91.7–98.4% dead tissue of brown seaweed.
Bioadsorption of heavy metals
Heavy metal removal rate from wastewater by algal materials was measured at different time intervals (1–5 h) under natural environmental conditions. All tested heavy metals were influenced by the biological treatment of wastewater, which showed a significant reduction in concentration. As shown in Fig. 3, the removal percentage of heavy metals increased with contact time. The higher removal percentage of all heavy metals by three algal materials is achieved at 3 h, and then an increase in contact time beyond 3 h had a negligible improvement in the removal percentage. The order of metal removal by the three algal materials is as the following: U. intestinalis Mg > Zn > Fe > Cu > Pb > Cr > Cd = Mn > Ni, S. latifolium Mg > Zn > Cu > Fe > Cr > Pb > Cd > Mn > Ni, C. officinalis Mg > Zn > Cu > Fe > Pb > Cr > Cd > Mn > Ni.
S. latifolium possessed the highest removal potential for tested metals followed by C. officinalis and then U. intestinalis which attained the lowermost removal potential. Whereas at 3 h, S. latifolium achieved removal percentages of 94.14, 92.62, 89.54, 87.54, 82.76, 80.95, 77.78, 73.02, and 71.62% of Mg, Zn, Cu, Fe, Cr, Pb, Cd, Mn, and Ni, respectively, while C. officinalis attained a removal percentage of 83.57, 81.15, 80.13, 76.56, 71.43, 68.96, 62.96, 60.32, and 56.76% of Mg, Zn, Cu, Fe, Pb, Cr, Cd, Mn, and Ni, respectively. U. intestinalis removed 80.12, 77.89, 75.40, 70.35, 66.67, 62.07, 55.57, 55.56, and 50% of Mg, Zn, Fe, Cu, Pb, Cr, Cd, Mn, and Ni, respectively, after 3 h.
One of the most important parameters influencing heavy metal removal as well as cost assessments is contact time, especially with the application of the process under natural environmental conditions. Most frequently, the contact time analysis pattern reveals that heavy metal elimination increases early in the process. The adsorption that occurs physically or the exchange of ions that takes place on a solid adsorbent’s surface causes a significant discrepancy between the concentration of heavy metal in the solution and its value on the adsorbent surface. Increasing contact time would have no further impact because there are initially more free-binding sites available before they start to decline and stagnate as a result of a decreasing number of empty sites for binding metal ions on the surface (Redha 2020; Znad et al. 2022). Furthermore, Bai and Venkateswarlu (2018) demonstrated that the biosorption process slows with time, and the adsorbate forms a one-molecule-thick layer on the surface. Indeed, the nonliving algal biomass benefits from the quick adsorption process, making it an effective agent for real-world uses in adsorption units (Mohammed et al. 2019).
Our findings are in conformity with Tabaraki et al. (2014), who found that the optimum contact time for adsorption Pb(II) by Sargassum ilicifolium was 200 min, and also Singh (2007) found that the maximum percentage removal of Ni(II) by dried biomass of Oscillatoria sp. and Spirogyra was achieved at 120 min, while Shyamala Devi et al. (2010) reported that the contact time 240 min was considered the equilibrium time for removing mercury by Sphaeroplea dried biomass. In addition, our results agree with El-Sheekh et al. (2022), who reported that with the help of produced silver nanoparticles from Sargassum latifolium and its aqueous extract, the adsorption of Fe+2 reached equilibrium after 150 min. The initial concentration of metal ions significantly impacts the algal biomass’s ability to biosorb heavy metal ions from aqueous solutions. With an increase in the initial metal ion concentration, the algal biomasses’ biosorption capacity for heavy metal removal improved. This can be due to the stronger driving force that elevated metal ion concentration provides, which beats on mass transfer resistances shown between the biosorbent and the aqueous solution (Rangabhashiyam and Balasubramanian 2018). This may explain why the three algal materials remove metals in the same order as they were initially concentrated in the effluent. Due to its large abundance in wastewater, magnesium takes the top spot among the three algal materials in the removal of metals.
Znad et al. (2022) reported that seaweed has excellent potential for metal removal. The biosorption of various heavy metal ions seems to depend on the type of algae used as well as the conditions under which the processes were conducted. When it comes to macro-algae, the cell walls of brown algae are mostly made of cellulose, alginic acid, polymers (such as mannuronic and guluronic acids) that are complexed with light metals (like sodium, potassium, and calcium), and polysaccharides (e.g., fucoidan). Fucoidan and alginate can bind metals via the exchange of ions, and the primary biosorption binding sites in alginate are carboxyl groups, followed by sulfate groups. Green algal cell walls mostly consist of proteins, which contain functional groups including amino, carboxyl, hydroxyl, and sulfate that aid in metal biosorption. On the other hand, red algae have cell walls primarily made of cellulose, but their ability to absorb nutrients comes from the presence of sulphated polysaccharides made by galactans (He and Chen 2014; Redha 2020).
The results proved that S. latifolium has the highest affinity of tested metal adsorption. Brown algae are said to have a higher metal uptake capability than red or green algae, and according to the Shamim (2018) research, many investigations also reported the superiority of brown algae as biosorbents for heavy metals. For instance, Sargassum wightii was found suitable for removing arsenic from an aqueous solution (Christobel and Lipton 2015); Sargassum polycystum was found by Jayakumar et al. (2021) as an effective biosorbent for cadmium and zinc removal.
Fourier‑transform infrared spectroscopy (FTIR)
FTIR technique was used to investigate the algal surface, and the three algae were also seen to undergo chemical alteration after wastewater treatment. Identification of the functional groups involved in the biosorption process is made possible through the analysis of the natural and enhanced biomass. Polysaccharides, proteins, and lipids, which are components of the algal cell wall, are the source of these groupings. Numerous functional groups, including carboxyl (such as fatty acids and amino acids), hydroxyl (such as polysaccharides), amine, phosphate, and sulfonate, among others, are available in the cell wall (Dmytryk et al. 2014). In the range of 4000–400 cm−1, FTIR spectra of the algae were collected before and after water treatment and are shown in Figs. 4, 5, and 6, which revealed a complex character of the biomass due to the presence of several peaks. After raw biomass and dirty water interacted, the peaks were seen to expand and move. The variations in algal cell wall characteristics, surface area, and surface charge density may cause variations in removal rates between the various species of algae (Gupta and Rastogi 2009). Ion exchange or physical adsorption at the cell surface may be used to remove wastes (Gupta and Rastogi 2009). The findings show that the functional groups on the surface wall of algae and the removed wastes exhibit high affinity and a powerful electrostatic force of attraction (Cengiz and Cavas 2008). Several polysaccharides in the algal cell walls are linked to proteins and other elements (Williams and Edyvean 1997; Tüzün et al. 2005). The functional groups on these molecules, which are present on the surface of algal cells, include carboxyl, amino, phosphate, thiol, and sulfhydryl groups (Tüzün et al. 2005).
The main absorption peaks, referred to as amine N–H and hydroxyl O–H stretching vibrations, show the presence of phenol or alcohol at wavenumbers between 3300 and 3400 cm−1 (Mohamed et al. 2021) at 2200–2900 cm−1 for all biomasses. –CH stretching vibration of C–CH3 occurs. It also referred to N = C = S stretching bond (Lu and Rasco 2012); at ~ 1700–1600 cm−1, the peak of C = O stretching mode of the amide I band occurs, and the absorption band of amide I occurs (Demir et al. 2015). The absorption peak at ~ 1400–1500 cm−1 represented the stretching of the C = O bond from carboxylic acids (O–H bending) (Younger 2014), in the range 1200–1300 cm−1 bending vibrations of C–H and O–H, rocking vibrations of CH2 (polysaccharides), and III amide band (proteins) occurs which assigned to cellulose, proteins, lipids, and sulfurous components (ElSaied et al. 2021). The absorption bands at 701–658 cm−1 show the stretching of C = S, which suggests sulfides in the tested algae (Touliabah, et al. 2022). The remaining spectra can primarily be attributed to cellulose, aliphatics of the cell wall, polysaccharides and monosaccharides, and aromatic chemicals. According to the FTIR findings, transmittance values were primarily shifted for carboxyl and hydroxyl groups. Additionally, it was observed that discrepancies between the peaks of the treated and untreated algal biomass were greater for Sargassum latifolium, which agrees with results obtained by GC, EDX, and SEM.
EDX
Using energy-dispersive X-ray (EDX) analysis, the effectiveness of the adsorption of heavy metals by seaweed was further confirmed. Figure 7 shows the typical EDX pattern for three algal materials before and after the sorption of tested heavy metals. It is noteworthy that EDX analysis enables to provide information about the composition of the adsorbent surface, the results (in terms of weight and atomic percentages) demonstrated the presence of C, O, and N, which are the main components of cellular macromolecules (Sultana, et al. 2020). The EDX pattern for the unloaded (native) U. intestinalis shows a signal of Mg ion, which increased obviously in metal-loaded E. intestinalis. Furthermore, the presence of Ca+2, which has been demonstrated participation in the ion exchange process with some metal ions, as indicated in the spectra of unloaded (native) U. intestinalis and was not detected in the EDX spectrum of loaded U. intestinalis. The EDX pattern for the unloaded (native) S. latifolium did not show the characteristic signal of Mg and Cu ions, whereas for the metal-loaded S. latifolium, the signals of these ions were observed. The EDX pattern for the unloaded (native) C. officinalis did not show the characteristic signal of Fe ion, whereas two signals of this ion were observed in the metal-loaded C. officinalis. In addition, the unloaded (native) S. latifolium shows a signal of Mg ion, which increased in metal-loaded S. latifolium.
SEM
The changes in cells’ surface morphology of U. intestinalis, S. latifolium, and C. officinalis biomasses in response to n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals adsorption were illustrated via SEM (Fig. 8). Before being exposed to contaminants, algal cells had a smooth, extremely porous structure that was hole-like (Fig. 8, Eb, Sb, and Cb). The surface of the biomass cells became rough and meandering after being exposed to n-alkanes, polycyclic aromatic hydrocarbons, and heavy metal ions due to the precipitation of contaminants ions surrounding the cell surface (Fig. 8, Ea, Sa, and Ca), These morphological alterations could be related to variations in the pores, morphology, and structure of algae’s cell walls. The cell wall of S. latifolium was very porous and ion permeable. This could explain why S. latifolium biomass has the highest affinity for n-alkane, polycyclic aromatic hydrocarbon, and heavy metal removal. Omar et al. (2018) and Michalak et al. (2018) observed modifications in algal surface porosity brought and induced by dye adsorption. Christobel and Lipton (2015) reported that SEM micrographs of different algal biomasses illustrate changes in cell morphology due to heavy metal removal.
Zeta potential (ζ)
The zeta potentials reveal that algal cells have a charge on the surface (mostly negatively charged) (Taki et al. 2008). Figure 9 shows that the surface charges of E. intestinalis, S. latifolium, and C. officinalis were − 14.3 ± 5.09 mV, − 22.0 ± 3.80 mV, and − 15.1 ± 3.52 mV, respectively; in this study, according to zeta potentials measurements, the cell surface of S. latifolium has a more negative surface charge than for U. intestinalis and C. officinalis. This agrees with our above gas chromatography-mass spectrometer (GC–MS) results, which showed that S. latifolium dry biomass has more potential as an adsorbent for light n-alkanes, heavy n-alkanes, and polycyclic aromatic hydrocarbons from wastewater than U. intestinalis and C. officinalis.
Conclusion
This research highlights the potential of seaweed dry biomasses for the use in bioadsorption of n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals from wastewater under natural environmental conditions. Different n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals ions were greatly influenced by the biological treatment of wastewater using U. intestinalis, S. latifolium, and C. officinalis, where they showed a high reduction in terms of concentration. The experiments were conducted at natural climatic conditions as a function of contact time. The optimum contact time for n-alkanes and polycyclic aromatic hydrocarbons was 15 days, while that for heavy metals removal was 3 h. S. latifolium was the best bioadsorbent for n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals from wastewater, followed by C. officinalis and U. intestinalis. According to this study, seaweed biomasses might make a straightforward, environmentally friendly, economically viable, and secure alternative biosorbent for fine-tuning wastewater treatment.
Data availability
Data will be made available on request.
References
Agüera A, Plaza-Bolaños P, Fernández FGA (2020) Removal of contaminants of emerging concern by microalgae-based wastewater treatments and related analytical techniques. In: Varjani S, Pandey A, Tyagi RD, Ngo HH, Larroche C (eds) Current developments in biotechnology and bioengineering. Elsevier, pp 503–525
Ahmad I, Abdullah N, Koji I, Yuzir A, Mohamad SE (2021) Potential of microalgae in bioremediation of wastewater. Bull Chem React Eng Catal 16:413–429
Ahmad A, Banat F, Alsafar H, Hasan SW (2022) Algae biotechnology for industrial wastewater treatment, bioenergy production, and high-value bioproducts. Sci Total Environ 806:150585
Ahmed SF, Mofijur M, Parisa TA, Islam N, Kusumo F, Inayat A, Le VG, Badruddin IA, Khan TMY, Ong TMY (2022) Progress and challenges of contaminate removal from wastewater using microalgae biomass. Chemosphere 286:131656
Akl FM, Ahmed SI (2022) Factors affecting removal of polycyclic aromatic hydrocarbons from seawater by dry brown seaweed Padina pavonica. Indian Streams Res J 12(10):1–13. https://doi.org/10.9780/22307850, http://isrj.org/UploadedData/10578.pdf
Alazaiza MY, Albahnasawi A, Mohammed ZA, Bashir JK, Al-Wahaibi T, Abujazar MS, Abu Amr SS, Nassani DE (2022) Potential use of algae for the bioremediation of different types of wastewater and contaminants: production of bioproducts and biofuel for green circular economy. J Environ Manag 324:116415
Aleem AA (1978) Contribution to the study of the marine algae of the red sea. I-The algae in the neighborhood of al-Ghardaqa, Egypt (Cyanophyceae, Chlorophyceae and Phaeophyceae). Bull Faculty Sci King Abdulaziz Univ Jeddah 2:73–88
Aleem AA (1991) Marine algae in Alexandria, Egypt. Alexandria Privately Published, Alexandria, VA, USA, pp 1–135
Alkio M, Tabuchi TM, Wang X, Colon-Carmona A (2005) Stress responses to polycyclic aromatic hydrocarbons in Arabidopsis include growth inhibition and hypersensitive response-like symptoms. J Exp Bot 56:2983–2994
Bai MT, Venkateswarlu P (2018) Fixed bed and batch studies on biosorption of lead using Sargassum tenerrimum powder: characterization, kinetics and thermodynamics. Materi Today Proceedings 5(9):18024–18037. https://doi.org/10.1016/j.matpr.2018.06.136
Basant EF, Amany MD, Ahmed AT, Mousa AA, Moussa S (2021) Potent antibacterial action of phycosynthesized selenium nanoparticles using Spirulina platensis extract. Green Process Synth 10:49–60
Bhatt P, Bhandari G, Bhatt K, Simsek H (2022) Microalgae-based removal of pollutants from wastewaters: occurrence, toxicity and circular economy. Chemosphere 306:135576
Blanco-Vieites M, Suárez-Montes D, Delgado F, Álvarez-Gil M, Battez AH, Rodríguez E (2022) Removal of heavy metals and hydrocarbons by microalgae from wastewater in the steel industry. Algal Res 64:102700
Briffa J, Sinagra E, Blundell R (2020) Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon 6(9):e04691. https://doi.org/10.1016/j.heliyon.2020.e04691
Cengiz S, Cavas L (2008) Removal of methylene blue by invasive marine seaweed: Caulerpa racemosa var. cylindracea. Biores Technol 99:2357–2363
Chen X, Cui J, Xu X, Sun B, Zhang L, Dong W, Chen C, Sun D (2020) Bacterial cellulose/attapulgite magnetic composites as an efficient adsorbent for heavy metal ions and dye treatment. Carbohyd Polym 229:115512
Cheng SY, Show PL, Lau BF, Chang JS, Ling TC (2019) New prospects for modified algae in heavy metal adsorption. Trends Biotechnol 37:1255–1268
Christobel J, Lipton AP (2015) Evaluation of macroalgal biomass for removal of heavy metal arsenic (As) from aqueous solution. Int J Appl Innov Eng Manag 4:94–104
Chu L, Hou X, Song X, Zhao X (2022) Toxicological effects of different ionic liquids on growth, photosynthetic pigments, oxidative stress, and ultrastructure of Nostoc punctiforme and the combined toxicity with heavy metals. Chemosphere 298:134273
Chung MK, Martin TK, Cheung KC, Nora FY, Wong MH (2007) Removal of aqueous phenanthrene by brown seaweed Sargassum hemiphyllum: sorption-kinetic and equilibrium studies. Sep Purif Technol 54:355–362
Demir P, Onde S, Severcan F (2015) Phylogeny of cultivated and wild wheat species using ATR-FTIR spectroscopy. Spectrochim Acta Part A Mol Biomol Spectrosc 135:757–763. https://doi.org/10.1016/j.saa.2014.07.025
Dmytryk A, Saeid A, Chojnacka K (2014) Biosorption of microelements by Spirulina: towards technology of mineral feed supplements. Sci World J 356328:1–15. https://doi.org/10.1155/2014/356328
Du T, Bogush A, Edwards P, Stanley P, Lombardi AT, Campos LC (2022) Bioaccumulation of metals by algae from acid mine drainage: a case study of Frongoch Mine (UK). Environ Sci Pollut Res 29(21):32261–32270
Dubey S, Chen CW, Haldar D, Tambat VS, Kumar P, Tiwari A, Singhania R, Dong CD, Patel AK (2023) Advancement in algal bioremediation for organic, inorganic, and emerging pollutants. Environ Pollut 317:120840
El Maghraby DM, Hassan IA (2021) Photosynthetic and biochemical response of Ulva lactuca to marine pollution by polyaromatic hydrocarbons (PAHs) collected from different regions in Alexandria City, Egypt. Egypt J Bot 61:467–478
ElSaied BEF, Diab AM, Tayel AA, Alghuthaymi MA, Moussa SH (2021) Potent antibacterial action of phycosynthesized selenium nanoparticles using Spirulina platensis extract. Green Process Synth 10(1):49–60. https://doi.org/10.1515/gps-2021-0005
El-Sheekh MM, Deyab MA, Hassan NI, Abu Ahmed SE (2022) Bioadsorption of Fe (II) ions from aqueous solution using Sargassum latifolium aqueous extract and its synthesized silver nanoparticles. Int J Phytorem 25(9):1234–1247. https://doi.org/10.1080/15226514.2022.2145000
El-Shoubaky GA, Mohammad SH (2016) Bioaccumulation of gasoline in brackish green algae and popular clams. Egypt J Aquat Res 42(1):91–98. https://doi.org/10.1016/j.ejar.2015.07.005
Esfandiar N, Suri R, McKenzie ER (2021) Simultaneous removal of multiple polycyclic aromatic hydrocarbons (PAHs) from urban stormwater using low-cost agricultural/industrial byproducts as sorbents. Chemosphere 274:129812
Ghodrati M, Zarrini G, Kosari-Nasab M, Movafeghi A (2022) Capability of the microalga Scenedesmus dimorphus for biodegradation of crude oil components: biological responses and catabolic intermediates. Clean: Soil, Air, Water 50:2200207
Guiry GM (2020) Algae base; World‑Wide Electronic Publication; National University of Ireland: Galway, Ireland, Available online: https://www.algaebase.org Accessed on 20 October 2022
Gupta VK, Rastogi A (2009) Biosorption of hexavalent chromium by raw and acid treated green alga Oedogonium hatei from aqueous solutions. J Hazard Mater 163:396–402
Haiba NS (2019) Polycyclic aromatic hydrocarbons (PAHs) in the River Nile, Egypt: occurrence and distribution. Polycyclic Aromat Compd 39(5):425–433
Haiba NS, Asaal AM, El Massry A, Ismail I, Basahi J, Hassan IA (2019) Effects of “Doneness” level on PAH concentrations in charcoal-grilled beef and chicken: an Egyptian study case. Polycyclic Aromat Compd 41(8):1–11
He J, Chen JP (2014) A comprehensive review on biosorption of heavy metals by algal biomass: materials, performances, chemistry, and modeling simulation tools. Biores Technol 160:67–78. https://doi.org/10.1016/j.biortech.2014.01.068
Huang J, Zhang Y, Bing H, Peng J, Dong F, Gao J, Arhonditsis GB (2021) Characterizing the river water quality in China: recent progress and on-going challenges. Water Res 201:117309
Jaafari J, Yaghmaeian K (2019) Optimization of heavy metal biosorption onto freshwater algae (Chlorella coloniales) algae cells using response surface methodology (RSM). Chemosphere 217:447–455
Jayakumar V, Govindaradjane S, Rajamohan N, Rajasimman M (2021) Biosorption potential of brown algae, Sargassum polycystum, for the removal of toxic metals, cadmium and zinc. Environ Sci Pollut Res 29:41909–41922. https://doi.org/10.1007/s11356-021-15185-7
Khalid S, Shahid M, Natasha BI, Sarwar T, Shah AH, Niazi NK (2018) A review of environmental contamination and health risk assessment of wastewater use for crop irrigation with a focus on low and high-income countries. Int J Environ Res Public Health 15(5):895. https://doi.org/10.3390/ijerph15050895
Kottuparambil S, Agusti S (2020) Cell-by-cell estimation of PAH sorption and subsequent toxicity in marine phytoplankton. Chemosphere 259:127487
Kumar V, Parihar RD, Sharma A, Bakshi P, Sidhu GPS, Bali AS, Karaouzas I, Bhardwaj R, Thukral AK, Gyasi-Agyei Y et al (2019) Global evaluation of heavy metal content in surface water bodies: a meta-analysis using heavy metal pollution indices and multivariate statistical analyses. Chemosphere 236:124364
Lipkin Y, Silva P (2002) Marine algae and seagrasses of the Dahlak Archipelago, southern Red Sea. Nova Hedwig 75:1–90
Lu X, Rasco BA (2012) Determination of antioxidant content and antioxidant activity in foods using infrared spectroscopy and chemometrics: a review. Crit Rev Food Sci Nutr 52(10):853–875. https://doi.org/10.1080/10408398.2010.511322
Michalak I, Mironiuk M, Marycz K (2018) A comprehensive analysis of biosorption of metal ions by macroalgae using ICP-OES, SEM-EDX and FTIR Techniques. Plos One 13(10):1371
Mohamed R, Fawzy E, Shehab R, Ali D, Salah R, Abd H (2021) Green biosynthesis, structural characterization and anticancer activity of copper oxide nanoparticles from the brown alga cystoseira myrica. Egypt J Aquat Biol Fish 25:341–358. https://doi.org/10.21608/ejabf.2021.189069
Mohammed AA, Najim AA, Al-Musawi TJ, Alwared AI (2019) Adsorptive performance of a mixture of three nonliving algae classes for nickel remediation in synthesized wastewater. J Environ Health Sci Eng 17:529–538
Nathana RJ, Jain AK, Rosengren RJ (2022) Biosorption of heavy metals from water: mechanism, critical evaluation and translatability of methodology. Environ Technol Rev 11(1):91–117. https://doi.org/10.1080/21622515.2022.2078232
Ofrydopoulou A, Nannoua C, Evgenidoua E, Christodoulou A, Lambropoulou D (2022) Assessment of a wide array of organic micropollutants of emerging concern in wastewater treatment plants in Greece: occurrence, removals, mass loading and potential risks. Sci Total Environ 802:149860
Omar H, El-Gendy A, Al-Ahmary K (2018) Bioremoval of toxic dye by using different marine macroalgae. Turk J Bot 42:1703
Premnath N, Mohanrasu K, Rao R, Dinesh GH, Prakash GH, Ananthi V, Ponnuchamy K, Muthusamy G, Arun A (2021) A crucial review on polycyclic aromatic hydrocarbons-environmental occurrence and strategies for microbial degradation. Chemosphere 280:130608
Qari HA, Hassan IA (2017) Bioaccumulation of PAHs in Padina boryana alga collected from a contaminated site on the Red Sea, Saudi Arabia. Pol J Environ Stud 26:435–442
Rajkumar K, Mvs S, Koganti S, Burgula S (2020) Selenium nanoparticles synthesized using pseudomonas stutzeri (MH191156) show antiproliferative and antiangiogenic activity against cervical cancer cells. Int J Nanomed 23:4523–4540
Rangabhashiyam S, Balasubramanian P (2018) Characteristics, performances, equilibrium and kinetic modeling aspects of heavy metal removal using algae. Bioresour Technol Rep 5:261–279. https://doi.org/10.1016/j.biteb.2018.07.009
Redha AA (2020) Removal of heavy metals from aqueous media by biosorption. Arab J Basic Appl Sci 27:183–193
Rehman K, Fatima F, Waheed I, Akash MSH (2018) Prevalence of exposure of heavy metals and their impact on health consequences. J Cell Biochem 119(1):157–184. https://doi.org/10.1002/jcb.26234
Rocha GS, Parrish CC, Espíndola EL (2020) Shifts in photosynthetic parameters and lipid production of the freshwater microalga Selenastrum gracile (Chlorophyceae) under cadmium exposure. J Appl Phycol 32:4047–4055
Sánchez AL, Gálvez AS, Juárez OA, Guerrero CS, Nunnelly DA, Nieves DC, Hernández MS (2022) Microalgae-based livestock wastewater treatment (MbWT) as a circular bioeconomy approach: enhancement of biomass productivity, pollutant removal and high-value compound production. J Environ Manag 308:114612
Satya ADM, Cheah WY, Yazdi SK, Cheng YS, Khoo KS, Vithanage M, Show PL (2023) Progress on microalgae cultivation in wastewater for bioremediation and circular bioeconomy. Environ Res 218:114948
Banerjee A (2021) Toxic effect and bioremediation of oil contamination in algal perspective, In: Kumar V, Saxena, G, Shah MP (eds) Bioremediation for environmental sustainability. Elsevier, pp 283–298
Shamim S (2018) Biosorption of heavy metals. Biosorption InTechopen. https://doi.org/10.5772/intechopen.72099
Shrestha R, Ban S, Devkota S, Sharma S, Joshi R, Tiwari A, Kim H, Joshi M (2021) Technological trends in heavy metals removal from industrial wastewater: a review. J Ind Eng Chem 9:105688. https://doi.org/10.1016/j.jece.2021.105688
Shyamala Devi B, John MS, Rajendran S, Manimaran N, Rengan P, Jayasundari J, Mannivannan M (2010) Removal of mercury by biosorption onto Sphaeroplea algae. Zaštita Materijala 51:227–231
Singh D (2007) Removal of Ni(II) from aqueous solution by biosorption using two green algal species Oscillatoria sp. and Spirogyra sp. 5th WSEAS Int. Conf. on Environment, Ecosystems and Development. Tenerife, Spain, pp 310–314
Sultana N, Hossain SM, Mohammed ME, Irfan M, Bashirul H, Faruque MO, Razzak SA, Hossain M (2020) Experimental study and parameters optimization of microalgae based heavy metals removal process using a hybrid response surface methodology-crow search algorithm. Sci Rep 10:15068. https://doi.org/10.1038/s41598-020-72236-8
Tabaraki R, Nateghi A, Asbchin SA (2014) Biosorption of lead (II) ions on Sargassum ilicifolium: application of response surface methodology. Int Biodeterior Biodegrad 93:145–152
Taki K, Seki T, Mononobe S, Kato K (2008) Zeta potential measurement on the surface of blue-green algae particles for micro-bubble process. Water Sci Technol 57(1):19–25
Tang W, He M, Chen B, Ruan G, Xia Y, Xu P, Song G, Bi Y, Hu B (2023) Investigation of toxic effect of mercury on Microcystis aeruginosa: correlation between intracellular mercury content at single cells level and algae physiological responses. Sci Total Environ 858:159894
Tomar RS, Jajoo A (2021) Enzymatic pathway involved in the degradation of fluoranthene by microalgae Chlorella vulgaris. Ecotoxicology 30:268–276
Touliabah HE, El-Sheekh MM, Makhlof MEM (2022) Evaluation of Polycladia myrica mediated selenium nanoparticles (PoSeNPS) cytotoxicity against PC-3 cells and antiviral activity against HAV HM175 (Hepatitis A), HSV-2 (Herpes simplex II), and adenovirus strain 2. Front Mar Sci 9:1092343. https://doi.org/10.3389/fmars.2022.1092343
Tsekova K, Todorova D, Ganeva S (2010) Removal of heavy metals from industrial wastewater by free and immobilized cells of Aspergillus niger. Int Biodeterior Biodegrad 64:447–451. https://doi.org/10.1016/j.ibiod.2010.05.003
Tüzün İ, Bayramoğlu G, Yalçın E, Başaran G, Çelik G, Arıca MY (2005) Equilibrium and kinetic studies on biosorption of Hg (II), Cd (II) and Pb (II) ions onto micro algae Chlamydomonas reinhardtii. J Environ Manag 77:85–92
United Nations Educational, Scientific and Cultural Organization [UNESCO] (2021) World water development report, http://www.unesco.org/new/en/naturalsciences/environment/water/wwap/wwdr/. Accessed 18 August 2021
Williams CJ, Edyvean RGJ (1997) Ion exchange in nickel biosorption by seaweed materials. Biotechnol Progr 13:424–428
Wrabel LM, Peckol P (2000) Effects of bioremediation on toxicity and chemical composition of no. 2 fuel oil: growth responses of the brown alga Fucus vesiculosus. Mar Pollut Bull 40:135–139
Xia L, Zhao Z, Lang Z, Qin Z, Zhu Y (2022) Exploring the cumulative selectivity of polycyclic aromatic hydrocarbons in phytoplankton, water and sediment in typical urban water bodies. Water 14:3145
Younger P (2014) The Merck index, 15th edn. Emerald Group Publishing Limited, London
Yuan Y, Wu Y, Ge X, Nie D, Wang M, Zhou H, Chen M (2019) In vitro toxicity evaluation of heavy metals in urban air particulate matter on human lung epithelial cells. Sci Total Environ 678:301–308. https://doi.org/10.1016/j.scitotenv.2019.04.431
Zeng G, He Y, Liang D, Wang F, Luo Y, Yang H, Wang Q, Wang J, Gao P, Wen X, Yu C, Sun D (2022) Adsorption of heavy metal ions copper, cadmium and nickel by Microcystis aeruginosa. Int J Environ Res Public Health 19(21):13867. https://doi.org/10.3390/ijerph192113867.PMID:36360745;PMCID:PMC9656734
Zhang T, Ruan J, Zhang B, Lu S, Gao C, Huang L, Bai X, Xie L, Gui M, Qiu RL (2019) Heavy metals in human urine, foods and drinking water from an e-waste dismantling area: identification of exposure sources and metal-induced health risk. Ecotoxicol Environ Saf 169:707–713
Znad H, Awual MR, Martini S (2022) The utilization of algae and seaweed biomass for bioremediation of heavy metal-contaminated wastewater. Molecules 27:1275. https://doi.org/10.3390/molecules27041275
Funding
Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).
Author information
Authors and Affiliations
Contributions
Fauza Akl: conceptualization, data curation, formal analysis, funding acquisition, investigation, and methodology; Suzan Ahmad: original draft, data curation, and review and editing; Mostafa El-Sheekh: investigation, writing, and review and editing; Mofida Makhlof: investigation, editing, formal analysis, methodology, resources, validation, visualization, and writing—review and editing. All authors have read the manuscript and approved it.
Corresponding author
Ethics declarations
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent to publish
Not applicable.
Competing interests
The authors declare no competing interests.
Additional information
Responsible Editor: Elena Maestri
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Akl, F.M.A., Ahmed, S.I., El-Sheekh, M.M. et al. Bioremediation of n-alkanes, polycyclic aromatic hydrocarbons, and heavy metals from wastewater using seaweeds. Environ Sci Pollut Res 30, 104814–104832 (2023). https://doi.org/10.1007/s11356-023-29549-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-023-29549-8