Abstract
Biological treatment of swine liquid manure may be a favorable environment for the enrichment of bacteria carrying antibiotic resistance genes (ARGs), raising the alert about this public health problem. The present work sought to investigate the performance of a swine wastewater treatment plant (SWWTP), composed of a covered lagoon biodigester (CLB) followed by three facultative ponds, in the removal of usual pollutants, antibiotics, ARGs (blaTEM, ermB, qnrB, sul1, and tetA), and intI1. The SWWTP promoted a 70% of organic matter removal, mainly by the digester unit. The facultative ponds stood out in the solids’ retention carried from the anaerobic stage and contributed to ammonia volatilization. The detected antibiotic in the raw wastewater was norfloxacin (< 0.79 to 60.55 μg L−1), and the SWWTP seems to equalize peaks of norfloxacin variation probably due to sludge adsorption. CLB reduced the absolute abundance of ARGs by up to 2.5 log, while the facultative stage does not seem to improve the quality of the final effluent in terms of resistance elements. Considering the relative abundances, the reduction rates of total and ARG-carrying bacteria appear to be similar. Finally, correlation tests also revealed that organic matter and solids control in liquid manure treatment systems could help reduce the spread of ARGs after the waste final disposal.
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Acknowledgements
The authors would also like to thank Prof. Juliana Calabria de Araújo, from the Microbiology Laboratory of the Department of Sanitary and Environmental Engineering at the Federal University of Minas Gerais, for providing the positive controls for qPCR.
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This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES – financing code 001), Brazilian National Health Foundation (FUNASA- grand number 2510001557612017-21), and National Council for Research and Scientific Development (CNPq – grand number 423101/2018-8).
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Andressa R Pereira: conceptualization, methodology, data curation, software, formal analysis, validation, investigation, and writing—original draft; Lucimeire A B Fonseca: methodology, investigation; Aline G O Paranhos: investigation, methodology, writing—review and editing; Camila C R F Cunha: investigation, methodology, writing—review and editing; Sérgio F Aquino: visualization, writing—review and editing, supervision, resources, funding acquisition; Silvana Q Silva: conceptualization, visualization, writing—review and editing, project administration, supervision, resources, funding acquisition.
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Pereira, .R., de Ávila Barbosa Fonseca, L., Paranhos, A.G. et al. Role of a typical swine liquid manure treatment plant in reducing elements of antibiotic resistance. Environ Sci Pollut Res 30, 91803–91817 (2023). https://doi.org/10.1007/s11356-023-28823-z
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DOI: https://doi.org/10.1007/s11356-023-28823-z