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Characterization of a cadmium-resistant functional bacteria (Burkholderia sp. SRB-1) and mechanism analysis at physiochemical and genetic level

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Abstract

In this study, the capacity of cadmium (Cd)-resistant plant growth-promoting bacteria (PGPB) Burkholderia sp. SRB-1 (SRB-1) and its mechanisms were explored through morphological characterizations, biochemical response, plant growth-promoting traits, and functional gene expression patterns. The results showed that SRB-1 was an excellent Cd-resistant bacteria (MIC was 420 mg L−1), and its maximum Cd removal rate reached 72.25%. Biosorption was the main removal method of Cd for SRB-1, preventing intracellular Cd accumulation and maintaining cellular metabolism. Various functional groups on the cell wall were involved in Cd binding, which deposited as CdS and CdCO3 on the cell surface according to XPS analysis and might be critical for reducing Cd physiochemical toxicity. Furthermore, metals exporting (zntA, czcA, czcB, czcC), detoxification (dsbA, cysM), and antioxidation (katE, katG, SOD1) related genes were annotated in the SRB-1 genome. The results of Cd distribution and antioxidative enzyme activity in SRB-1 also illustrated that Cd2+ efflux and antioxidative response were the main intracellular Cd-resistant mechanisms. These conclusions were further verified by qRT-PCR analysis. Overall, the strategies of extracellular biosorption, cation efflux, and intracellular detoxification jointly build the Cd-resistant system, which invested Burkholderia sp. SRB-1 with potential for bioremediation in heavily Cd-contaminated environmental sites.

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Acknowledgements

The authors also wish to thank Professor Guanglei Cheng and Hui Wang from Sichuan University for the technical assistance.

Funding

This work was financially supported by the National Key Research and Development Program of China (2018YFC1802605, 2020YFC1807703) and the Regional Innovation Cooperation Project of Sichuan Province (2021YFQ0014).

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  1. Huakang Liu and Huayan Huang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu, 610065, Sichuan, People’s Republic of China

    Huakang Liu, Huayan Huang, Ke Liang, Kangkai Lin & Heng Xu

  2. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China

    Yuxian Shangguan

  3. Key Laboratory of Environment Protection, Soil Ecological Protection and Pollution Control, Sichuan University & Department of Ecology and Environment of Sichuan, Chengdu, 610065, Sichuan, People’s Republic of China

    Heng Xu

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  1. Huakang Liu
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  3. Ke Liang
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Contributions

Huakang Liu: conceptualization, investigation, methodology, data curation, writing—original draft, writing—review and editing, visualization, supervision. Huayan Huang: investigation, methodology, data curation, writing—review and editing, visualization. Ke Liang: investigation, methodology, visualization. Kangkai Lin: visualization, data curation. Yuxian Shangguan: writing—review and editing, supervision, investigation, funding acquisition. Heng Xu: revising and editing, data processing, supervision, funding acquisition.

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Correspondence to Heng Xu.

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Liu, H., Huang, H., Liang, K. et al. Characterization of a cadmium-resistant functional bacteria (Burkholderia sp. SRB-1) and mechanism analysis at physiochemical and genetic level. Environ Sci Pollut Res 30, 78408–78422 (2023). https://doi.org/10.1007/s11356-023-27824-2

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