Abstract
Pluronics, due to its high water-soluble and thermoreversible ability, attracted much in biomedical applications. They are mainly utilized in drug delivery, gene therapy, and tissue remodeling. The study aims to explore the cytocompatibility of Pluronics F-127, which has gained much popularity due to its various properties. The cells were exposed to varying concentrations of Pluronics F-127 in A549 cells for 24 h. According to the MTT and neutral red assay, A549 cells displayed dose-dependent cell viability. The cell’s morphology was preserved after treatment, as seen in phase-contrast and Giemsa staining. When exposed to PF-127, lysosomal, cytoskeletal, and nuclear integrity were maintained. The percentage of live cells in all the treated groups was more significant than 90%, according to the live/dead flow cytometric analyses. The study identified the cytocompatibility of Pluronics F-127 required for the breakthrough in biomedical applications.
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Acknowledgements
The authors wish to express their thanks to the Director and Head, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology (Govt. of India), Trivandrum, Kerala, India, for their support and for providing the infrastructure to carry out this work. Ms Swathi S. thanks the Department of Science and Technology, New Delhi, for DST-SCTIMST Summer Scholarships Programme for students at the UG level.
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Megha: Draft, literature search, data collection, data analysis, and interpretation; Swathi: literature search, data collection, and data analysis; Joseph and Vandana: literature search, data collection, and data analysis; Mohanan: design, data analysis, interpretation, and final approval.
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Sundaresan Swathi attended the DST-SCTIMST Summer Scholarship Program for students at the UG level.
Highlights
• MTT assay for cell viability on exposure to Pluronics F-127.
• Detection of nuclear condensation using DAPI staining.
• Evaluation of cytoskeleton intactness using Rhodamine-phalloidin staining.
• Flow cytometric analysis of the live-dead assay using calcein AM-PI.
• Acridine-orange staining for assessing lysosomal integrity.
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Megha, K.B., Swathi, S., Joseph, X. et al. Cytocompatibility of Pluronics F-127 on adenocarcinomic human alveolar basal epithelial cells (A549 cells). Environ Sci Pollut Res 29, 71124–71135 (2022). https://doi.org/10.1007/s11356-022-20925-4
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DOI: https://doi.org/10.1007/s11356-022-20925-4