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Biochemical profiling of lead-intoxicated impaired lipid metabolism and its amelioration using plant-based bioactive compound

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Abstract

The aim of this study was to investigate the lead (Pb)-induced lipid metabolism impairment and its amelioration using plant-based therapeutic interventions. Pb-induced hepatotoxicity can disturb the normal levels of natural antioxidant enzymes including glutathione (GSH) and superoxide dismutase (SOD) exerting a crucial impact on membrane unsaturated fatty acids (FA), hence leading to lipid peroxidation. Furthermore, Pb toxicity can also alter the regulation of various hormones involved in the synthesis of 3-hydroxy-methyl glutaryl CoA (HMG-CoA reductase), leading to an impairment in normal levels of serum cholesterol and other associated conjugated lipid molecules such HDL-cholesterol, LDL-cholesterol and VLDL-cholesterol. In this study, the lipoprotein fractions, cholesterol, triglyceride (TGs) and biomarkers of liver functions were estimated by employing respective assay kits. The levels of antioxidant enzymes, FFAs and HMG-CoA reductase were determined by employing sandwich ELISA method. The administration of PbAc in experimental rats induced a significant disturbance in lipid profile (P < 0.05) accompanying a significant reduction in natural antioxidant defence system (P < 0.05). The significant alteration in the levels of serum antioxidant enzymes can lead to membrane lipid peroxidation that is reflected by a significantly (P < 0.05) high level of serum MDA in PbAc-induced experimental rats. However, the administration of resveratrol proved therapeutically effective in the treatment of Pb toxicity. Overall, the results of this study accompanying histopathological examination had proved the ameliorating effect of resveratrol in Pb-induced lipid metabolism impairment by adopting vitamin C as a standard therapeutic intervention.

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All data generated and/or analysed during this study are included in this published article.

Funding

This work has been financially supported by the research grants (8365/Punjab/NRPU/R&D/HEC/2017) received from the Higher Education Commission of Pakistan.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Government College University, Faisalabad, Pakistan

    Abdul Qader & Muhammad Sajid Hamid Akash

  2. Department of Pharmacy, University of Agriculture, Faisalabad, Pakistan

    Kanwal Rehman

Authors
  1. Abdul Qader
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  2. Kanwal Rehman
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  3. Muhammad Sajid Hamid Akash
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Contributions

AQ was involved in literature search, data curation, experimental analysis, investigation, writing the original draft and validation. KR was responsible for project administration, investigation, conceptualization, writing the final draft and editing. MSHA participated in project administration, supervision, conceptualization, methodology, writing the original draft preparation and editing.

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Correspondence to Muhammad Sajid Hamid Akash.

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This study was ethically approved from the Institutional Review Board (GCUF/IRB/4141) of Government College University Faisalabad (GCUF).

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The authors declare that they do not have any conflict of interest for this article.

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Responsible Editor: Gangrong Shi

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Qader, A., Rehman, K. & Akash, M.S.H. Biochemical profiling of lead-intoxicated impaired lipid metabolism and its amelioration using plant-based bioactive compound. Environ Sci Pollut Res 29, 60414–60425 (2022). https://doi.org/10.1007/s11356-022-20069-5

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  • DOI: https://doi.org/10.1007/s11356-022-20069-5

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