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The regulatory role of root in cadmium accumulation in a high cadmium-accumulating rice line (Oryza sativa L.)

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Abstract

There are some key processes that regulate cadmium (Cd) accumulation in rice. Understanding the characteristics and mechanisms of Cd accumulation in high Cd-accumulating rice lines benefits for excavating relevant genes. Cd accumulation and distribution in roots of Lu527-8, a high Cd-accumulating rice line, were investigated by a hydroponic experiment, with a control of a normal rice line (Lu527-4). Lu527-8 showed significantly higher Cd concentrations in roots than Lu527-4. More than 81% of Cd in roots of two rice lines is distributed in soluble fraction and cell wall. In soluble fraction, there were more organic acids, amino acids, and phytochelatins in Lu527-8, benefiting Cd accumulation. Pectin and hemicellulose 1 (HC1), especially pectin, were main polysaccharides in cell wall. Lu527-8 showed more pectin and HC1 along with higher pectin methylesterase (PME) activity compared with Lu527-4, promoting Cd accumulation. Besides, Lu527-8 showed higher Cd translocation from root to shoot due to more amounts of ethanol-extractable Cd in roots than Lu527-4. In conclusion, specific characteristics of Cd chemical forms and subcellular distribution in roots of high Cd-accumulating rice line are important for Cd accumulation and translocation.

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The data and materials will be available on request.

Abbreviations

Cd:

cadmium

PME:

pectin methylesterase

HC1:

hemicellulose 1

HC2:

hemicellulose 2

TF:

translocation factor

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Funding

This work was supported by Major Science and Technology Project of Sichuan Province (2018SZDZX0029), National Key Research and Development Program of China (2018YFC1802605), and National Natural Science Foundation of China (41807147).

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  1. Haiying Yu and Keji Wang are the co-first authors and made equal contributions to this work

Authors and Affiliations

  1. College of Resources, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China

    Haiying Yu, Keji Wang, Huagang Huang, Xizhou Zhang & Tingxuan Li

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  1. Haiying Yu
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  2. Keji Wang
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  3. Huagang Huang
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  4. Xizhou Zhang
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Contributions

Haiying Yu has contributed to conceptualization and writing (reviewing and editing). Keji Wang has contributed to formal analysis, visualization, and writing (original draft). Huagang Huang has contributed to methodology and software. Xizhou Zhang has contributed to supervision and validation. Tingxuan Li has contributed to supervision, resources, and writing (reviewing and editing). All authors read and approved the final manuscript.

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Correspondence to Tingxuan Li.

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Yu, H., Wang, K., Huang, H. et al. The regulatory role of root in cadmium accumulation in a high cadmium-accumulating rice line (Oryza sativa L.). Environ Sci Pollut Res 28, 25432–25441 (2021). https://doi.org/10.1007/s11356-021-12373-3

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