Abstract
In this study, it was aimed to determine the protective effects of melatonin (0.01, 0.1, and 1 mM) against 10 mg/L titanium dioxide nanoparticles (TiO2-NPs) on kinematic and oxidative indices in the sperm cells of Capoeta trutta. Therefore, TiO2 nanoparticles were synthesized primarily within the scope of the study. The synthesized nanoparticles were characterized by structurally different techniques. Then, melatonin and TiO2 were applied to Capoeta trutta sperm cells by in vitro. According to our data, all doses of melatonin showed protective effects on all velocities of sperm cells such as the straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) against TiO2-NPs, while 0.1 and 1 mM doses of melatonin improved the VSL value. Although TiO2-NPs increased total glutathione (tGSH), malondialdehyde (MDA) lipid peroxidation, and superoxide dismutase (SOD) compared to the control group, there were positive treatment effects for all doses of melatonin on antioxidant capacity of sperm cells. At the end of this research, it is suggested that over 0.1 mM dose of melatonin improves the velocity of sperm cells and it plays a protective role against the toxic effects of TiO2-NPs.
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Abbreviations
- TiO2-NPs:
-
titanium dioxide nanoparticles
- MEL:
-
melatonin
- TGSH:
-
total glutathione
- MDA:
-
malondialdehyde
- SOD:
-
superoxide dismutase
- VSL:
-
straight line velocity
- VCL:
-
curvilinear velocity
- VAP:
-
angular path velocity
- BCF:
-
beat cross frequency
- ALH:
-
amplitude of lateral displacement of the sperm cell head
- MAD:
-
mean angular displacement
- FTIR:
-
Fourier transform infrared spectrophotometer
- XRD:
-
X-ray diffract meter
- TGA:
-
thermo gravimetric analyzers
- DTA:
-
differential thermal analyzer
References
Abd El-Atti M, Desouky MMA, Mohamadien A, Said RM (2019) Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: bioaccumulation, oxidative stress and histopathological biomarkers. Egypt J Aquat Res 45:11–18. https://doi.org/10.1016/j.ejar.2019.01.001
Affonso FJ, Carvalho HF, Lançoni R et al (2017) Addition of antioxidants myoinositol, ferulic acid, and melatonin and their effects on sperm motility, membrane integrity, and reactive oxygen species production in cooled equine semen. J Equine Vet Sci. 59:57–63. https://doi.org/10.1016/j.jevs.2017.09.006
Afifi M, Saddick S, Abu Zinada OA (2016) Toxicity of silver nanoparticles on the brain of Oreochromis niloticus and Tilapia zillii. Saudi J Biol Sci 23:754–760. https://doi.org/10.1016/j.sjbs.2016.06.008
Akerboom TPM, Sies H (1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 77:373–382. https://doi.org/10.1016/S0076-6879(81)77050-2
Alagbonsi IA, Olayaki LA (2018) Melatonin attenuates Δ9-tetrahydrocannabinol-induced reduction in rat sperm motility and kinematics in-vitro. Reprod Toxicol 77:62–69. https://doi.org/10.1016/j.reprotox.2018.02.005
Amde M, Liu J, Tan Z-Q, Bekana D (2017) Transformation and bioavailability of metal oxide nanoparticles in aquatic and terrestrial environments. A review. Environ Pollut 230:250–267. https://doi.org/10.1016/j.envpol.2017.06.064
Appiah MO, He B, Lu W, Wang J (2019) Antioxidative effect of melatonin on cryopreserved chicken semen. Cryobiology. 89:90–95. https://doi.org/10.1016/j.cryobiol.2019.05.001
Bour A, Mouchet F, Silvestre J et al (2015) Environmentally relevant approaches to assess nanoparticles ecotoxicity: a review. J Hazard Mater 283:764–777. https://doi.org/10.1016/j.jhazmat.2014.10.021
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Chougala LS, Yatnatti MS, Linganagoudar RK et al (2017) A simple approach on synthesis of TiO2 nanoparticles and its application in dye sensitized solar cells. J Nano- Electron Phys 9(4):04005-1-04005-6. https://doi.org/10.21272/jnep.9(4).04005
Da Silva GH, Clemente Z, Khan LU et al (2018) Toxicity assessment of TiO2-MWCNT nanohybrid material with enhanced photocatalytic activity on Danio rerio (Zebrafish) embryos. Ecotoxicol Environ Saf 165:136–143. https://doi.org/10.1016/j.ecoenv.2018.08.093
Dawood MAO, Koshio S, Zaineldin AI, Doan H, Ahmed HA, Elsabagh M, Abdel-Daim MM (2019) An evaluation of dietary selenium nanoparticles for red sea bream (Pagrus major) aquaculture: growth, tissue bioaccumulation, and antioxidative responses. Environ Sci Pollut Res 26:30876–30884. https://doi.org/10.1007/s11356-019-06223-6
Esrefoglu M, Akinci A, Taslidere E et al (2016) Ascorbic acid and beta-carotene reduce stress-induced oxidative organ damage in rats. Biotech Histochem 91:455–464. https://doi.org/10.1080/10520295.2016.1220019
Gao Y, Yang C, Huang C et al (2019) The effects of melatonin supplement on paddlefish (Polyodon spathula) sperm quality and ATP content in sperm during in vitro storage. Aquaculture. 503:475–482. https://doi.org/10.1016/j.aquaculture.2019.01.010
Girardello F, Custódio Leite C, Vianna Villela I et al (2016) Titanium dioxide nanoparticles induce genotoxicity but not mutagenicity in golden mussel Limnoperna fortunei. Aquat Toxicol 170:223–228. https://doi.org/10.1016/j.aquatox.2015.11.030
Kandeil MA, Mohammed ET, Hashem KS, Aleya L, Abdel-Daim MM (2019) Moringa seed extract alleviates titanium oxide nanoparticles (TiO2-NPs)-induced cerebral oxidative damage, and increases cerebral mitochondrial. Environ Sci Pollut Res:1–16. https://doi.org/10.1007/s11356-019-05514-2
Karakilçik AZ, Bitiren M, Zerin M et al (2015) Melatonin increased vitamin C and antioxidant enzyme values in the plasma,heart, liver, and kidney of adriamycin-treated rats. Turkish J Biol 39:925–931. https://doi.org/10.3906/biy-1507-79
Lahnsteiner F, Mansour N, Plaetzer K (2010) Antioxidant systems of brown trout (Salmo trutta f. fario) semen. Anim Reprod Sci 119:314–321. https://doi.org/10.1016/j.anireprosci.2010.01.010
Lançoni R, Celeghini ECC, Alves MBR et al (2018) Melatonin added to cryopreservation extenders improves the mitochondrial membrane potential of postthawed equine sperm. J Equine Vet Sci 69:78–83. https://doi.org/10.1016/j.jevs.2018.06.006
Maitra SK, Hasan KN (2016) The role of melatonin as a hormone and an antioxidant in the control of fish reproduction. Front Endocrinol 7:38. https://doi.org/10.3389/fendo.2016.00038
Mansouri B, Johari SA, Azadi NA, Sarkheil M (2018) Effects of waterborne ZnO nanoparticles and Zn2+ions on the gills of rainbow trout (Oncorhynchus mykiss): bioaccumulation, histopathological and ultrastructural changes. Turkish J Fish Aquat Sci 18:739–746. https://doi.org/10.4194/1303-2712-v18_5_09
Mohammed ET, Safwat GM (2019) Grape seed proanthocyanidin extract mitigates titanium dioxide nanoparticle (TiO2-NPs)–induced hepatotoxicity through TLR-4/NF-κB signaling pathway. Biol Trace Elem Res:1–11. https://doi.org/10.1007/s12011-019-01955-5
Mohammed ET, Radi AM, Aleya L, Abdel-Daim MM (2019) Cynara scolymus leaves extract alleviates nandrolone decanoate-induced alterations in testicular function and sperm quality in albino rats. Environ Sci Pollut Res 27:5009–5017. https://doi.org/10.1007/s11356-019-07302-4
Okatani Y, Wakatsuki A, Kaneda C (2000) Melatonin increases activities of glutathione peroxidase and superoxide dismutase in fetal rat brain. J Pineal Res 28(2):89–96. https://doi.org/10.1034/j.1600-079X.2001.280204.x
Özgür ME, Balcıoğlu S, Ulu A et al (2018) The in vitro toxicity analysis of titanium dioxide (TiO2) nanoparticles on kinematics and biochemical quality of rainbow trout sperm cells. Environ Toxicol Pharmacol 62:11–19. https://doi.org/10.1016/j.etap.2018.06.002
Özgür ME, Okumuş F, Kocamaz AF (2019) A novel computer assisted sperm analyzer for assessment of spermatozoa motility in fish; BASA-sperm aqua. El-Cezeri fen ve Mühendislik Derg 6:208–219. https://doi.org/10.31202/ecjse.486342
Özkan N, Ersoy ÖF, Özsoy Z, Çakır E (2017) Melatonin exhibit supportive effects on oxidants and anastomotic healing during intestinal ischemia reperfusion injury. Turkish J Trauma Emerg Surg 24:1–8. https://doi.org/10.5505/tjtes.2017.23539
Patil NA, Gade WN, Deobagkar DD (2016) Epigenetic modulation upon exposure of lung fibroblasts to TiO2 and ZnO nanoparticles: alterations in DNA methylation. Int J Nanomedicine 11:4509–4519. https://doi.org/10.2147/IJN.S110390
Perumal P, Chang S, Baruah KK, Srivastava N (2018) Administration of slow release exogenous melatonin modulates oxidative stress profiles and in vitro fertilizing ability of the cryopreserved mithun (Bos frontalis) spermatozoa. Theriogenology. 120:79–90. https://doi.org/10.1016/j.theriogenology.2018.07.033
Poupard GP, Paxion C, Cosson J et al (1998) Initiation of carp spermatozoa motility and early ATP reduction after milt contamination by urine. Aquaculture 160:317–328. https://doi.org/10.1016/S0044-8486(97)00301-3
Samak DH, El-Sayed YS, Shaheen HM et al (2018) Developmental toxicity of carbon nanoparticles during embryogenesis in chicken. Environ Sci Pollut Res:1–15. https://doi.org/10.1007/s11356-018-3675-6
Shakeel M, Jabeen F, Shabbir S, Asghar MS, Khan MS, Chaudhry AS (2016) Toxicity of nano-titanium dioxide (TiO2-NP) through various routes of exposure: a review. Biol Trace Elem Res 172(1):1–36. https://doi.org/10.1007/s12011-015-0550-x
Song G, Gao Y, Wu H, Hou W, Zhang C, Ma H (2012) Physiological effect of anatase TiO2 nanoparticles on Lemna minor. Environ Toxicol Chem 31:2147–2152. https://doi.org/10.1002/etc.1933
Vignardi CP, Hasue FM, Sartório PV, Cardoso CM, Machado AS, Passos MJ, Santos TC, Nucci JM, Hewer TL, Watanabe IS, Gomes V, Phan NV (2015) Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766). Aquat Toxicol 158:218–229. https://doi.org/10.1016/j.aquatox.2014.11.008
Wang Z, Kong W, Liu B et al (2011) An experimental study on the shape changes of TiO2 nanocrystals synthesized by microemulsion-solvothermal method. J Nanomater. https://doi.org/10.1155/2011/467083
Zahin N, Anwar R, Tewari D, Kabir MT, Sajid A, Mathew B, Uddin MS, Aleya L, Abdel-Daim MM (2019) Nanoparticles and its biomedical applications in health and diseases: special focus on drug delivery. Environ Sci Pollut Res:1–18. https://doi.org/10.1007/s11356-019-05211-0
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Özgür, M.E., Ulu, A., Noma, S.A.A. et al. Melatonin protects sperm cells of Capoeta trutta from toxicity of titanium dioxide nanoparticles. Environ Sci Pollut Res 27, 17843–17853 (2020). https://doi.org/10.1007/s11356-020-08273-7
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DOI: https://doi.org/10.1007/s11356-020-08273-7