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In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary

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Abstract

The aim of the study was to evaluate cyto- and genotoxic effects in populations living in subnormal clusters in Santos São Vicente estuary. For in vivo study, samples of buccal mucosa and peripheral blood cells were collected. Micronucleus assay and single-cell gel (comet) assay were performed. For in vitro study, Chinese ovary hamster (CHO) cells were exposed to contaminated water. The results showed that people living in the contaminated estuary have increased DNA damage in oral mucosa and peripheral blood cells, as detected in the micronucleus and comet assays respectively. In addition, estuarine water was able to promote cytotoxicity at the highest concentrations, as well as decrease the number of cells in the G1 phase. In summary, our results indicate that water from the Santos-São Vicente estuary is capable of inducing cytogenotoxicity in mammalian cells in vivo and in vitro.

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References

  • Amorim LC (2003) Os biomarcadores e sua aplicação na avaliação da exposição aos agentes químicos ambientais. Rev Bras Epidemiol 6:158–170 [in Portuguese]

    Article  Google Scholar 

  • Azqueta A, SAS L, Boutet-Robinet E, Duthie S, Ladeira C, Møller P, Collins AR, Godschalk RWL, Working Group 5 of the hCOMET project (CA15132) (2019) DNA repair as a human biomonitoring tool: comet assay approaches. Mutat Res 781:71–87

    Article  CAS  Google Scholar 

  • Banci KR, Mori GM, Oliveira MA et al (2017) Can environmental pollution by metals change genetic diversity? Ucides cordatus (Linnaeus, 1763) as a study case in Southeastern Brazilian mangroves. Mar Pollut Bull 116(1–2):440–447

    Article  CAS  Google Scholar 

  • Belien JA, Copper MP, Braakhuis BJ et al (1995) Standardization of counting micronuclei: definition of a protocol to measure genotoxic damage in human exfoliated cells. Carcinogenesis 16(10):2395–2400

    Article  CAS  Google Scholar 

  • Beyersmann D, Hartwig A (2008) Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxicol 82(8):493–512

    Article  CAS  Google Scholar 

  • Biswas D, Sen G, Sarkar A et al (2011) Atorvastatin acts synergistically with N-acetyl cysteine to provide therapeutic advantage against Fas-activated erythrocyte apoptosis during chronic arsenic exposure in rats. Toxicol Appl Pharmcol 250(1):39–53

    Article  CAS  Google Scholar 

  • Bolognesi C, Knasmueller S, Nersesyan A, Thomas P, Fenech M (2013) The HUMNxl scoring criteria for different cell types and nuclear anomalies in the buccal micronucleus cytome assay - an update and expanded photogallery. Mutat Res 753:100–113

    Article  CAS  Google Scholar 

  • Cesar A, Pereira CDS, Santos AR, Others. (2006) Ecotoxicological assessment of sediments from the Santos and S{ã}o Vicente estuarine system-Brazil. Braz J Ocean 54(1):55–63

  • CETESB. Companhia de Tecnologia de Saneamento Ambiental. Relatório de águas costeiras, (2016) 181p. Available em:http://cetesb.sp.gov.br/praias/wp-content/uploads/sites/31/2013/11/relatorio-praias-2016.pdf[in Portuguese]

  • Choueri RB, Cesar A, Abessa DMS, Torres RJ, Morais RD, Riba I, Pereira CD, Nascimento MR, Mozeto AA, DelValls T (2009) (2009). Development of site-specific sediment quality guidelines for North and South Atlantic littoral zones: comparison against national and international sediment quality benchmarks. J Hazard Mater 170(1):320–331

    Article  CAS  Google Scholar 

  • Cordeiro CA, Costa TM (2010) Evaluation of solid residues removed from a mangrove swamp in the São Vicente estuary, SP, Brazil. Mar Pollut Bull 60(10):1762–1767

    Article  CAS  Google Scholar 

  • Costa C, Teixeira JP, Silva S, Roma-Torres J, Coelho P, Gaspar J, Alves M, Laffon B, Rueff J, Mayan O (2006) Cytogenetic and molecular biomonitoring of a Portuguese population exposed to pesticides. Mutagenesis 21(5):343–350

    Article  CAS  Google Scholar 

  • de Oliveira BF, Chacra AP, Frauches TS, Vallochi A, Hacon S (2014) A curated review of recent literature of biomarkers used for assessing air pollution exposures and effects in humans. J Toxicol Environ Health B Crit Rev 17(7–8):369–410

    Article  CAS  Google Scholar 

  • De Vizcaya-Ruiz A, Barbier O, Ruiz-Ramos R et al (2009) Biomarkers of oxidative stress and damage in human populations exposed to arsenic. Mutat Res 674(1–2):85–92

    Article  CAS  Google Scholar 

  • Dingemans MM, Baken KA, van der Oost R, Schriks M, van Wezel AP (2019) Risk-based approach in the revised European Union drinking water legislation: opportunities for bioanalytical tools. Integr Environ Assess Manag 15(1):126–134

    Article  Google Scholar 

  • Dorne JL, Kass GE, Bordajandi LR et al (2011) Human risk assessment of heavy metals: principles and applications. Metals Ions Life Sci 8:27–60

    CAS  Google Scholar 

  • Fernandes BS (2003) Nova abordagem para o grave problema da desnutrição infantil. Estudos Avançados 17(48):77–92 [in Portuguese]

    Article  Google Scholar 

  • Gimiliani GT, Fontes RFC, de Souza Abessa DM (2016) Modeling the dispersion of endocrine disruptors in the Santos Estuarine System (Sao Paulo State, Brazil). Brazilian J Oceanogr 64(1):1–8

    Article  Google Scholar 

  • Groten JP, Feron VJ, Sühnel J (2001) Toxicology of simple and complex mixtures. Trends Pharmacol Sci 22(6):316–322

    Article  CAS  Google Scholar 

  • Hernández AF, Tsatsakis AM (2017) Human exposure to chemical mixtures: challenges for the integration of toxicology with epidemiology data in risk assessment. Food Chem Toxicol 103:188–193

    Article  CAS  Google Scholar 

  • Hou H, Yu Y, Shen Z, Liu S, Wu B (2017) Hepatic transcriptomic responses in mice exposed to arsenic and different fat diet. Environ Sci Pollut Res 24(11):10621–10629

    Article  CAS  Google Scholar 

  • IBGE. Instituto Brasileiro de Geografia e Estatística. Censo (2010) Disponível em: [www.ibge.gov.br/home/estatistica/populacao/censo2010/]. Assessed em 05/09/2018 [in Portuguese]

  • Jiang X, Chen C, Zhao W, Zhang Z (2013) Sodium arsenite and arsenic trioxide differently affect the oxidative stress, genotoxicity and apoptosis in A549 cells: an implication for the paradoxical mechanism. Environ Toxicol Pharmacol 36(3):891–902

    Article  CAS  Google Scholar 

  • Kennish MJ (2002) Environmental threats and environmental future of estuaries. Enviorn Conserv 29(1):78–107

    Article  Google Scholar 

  • Kim BSM, Salaroli AB, de Lima Ferreira PA, Sartoretto JR, de Mahiques MM, Figueira RCL (2016) Spatial distribution and enrichment assessment of heavy metals in surface sediments from Baixada Santista, Southeastern Brazil. Mar Pollut Bull 103(1–2):333–338

    Article  CAS  Google Scholar 

  • Krämer O, Klausing S, Noll T (2010) Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches. Appl Microbiol Biotechnol 88(2):425–436

    Article  CAS  Google Scholar 

  • Neumann B, Vafeidis AT, Zimmermann J, Nicholls RJ (2015) Future coastal population growth and exposure to sea-level rise and coastal flooding-a global assessment. PLoS One 10(3):e0118571

    Article  CAS  Google Scholar 

  • Perina FC, Torres RJ, Mozeto AA, Nascimento MRL, Abessa DMS (2018) Sediment quality assessment of the tributaries of the Santos-São Vicente Estuarine System – Brazil. Ecotoxicol Environ Contam [Internet] 13(2):25–38 Available from: http://ecotoxbrasil.org.br/upload/a44358a45e8efe76c53b4398f1cfa78c-005-eec-12196-25-38.pdf

    Google Scholar 

  • Sampaio A, Mateus M, Ribeiro R (2008) Assessing the impact of several development scenarios on the water quality in Santos Estuary. In: Neves R, Baretta J, Mateus M (eds) Perspectives on integrated coastal zone management in South America. IST Press, Lisboa, pp 435–444

    Google Scholar 

  • Souza ACF, Yujra VQ, Pisani LP, De Barros VM, De Castro GM, Ribeiro DA (2019) The use of single-cell comet assay on oral cells: a critical review. Anticancer Res 39(8):4011–4017

    Article  Google Scholar 

  • Thomas P, Harvey S, Gruner T, Fenech M (2008) The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls. Mutat Res 638(1):37–47

    Article  CAS  Google Scholar 

  • Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu J.-C Sasaki YF (2000) Single cell gel/comet assay: guideline for in vitro and in vivo genetic toxicology testing. Environ Mol Mutag 35 (3): 206–221

  • Tolbert PE, Shy CM, Allen JW (1992) Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res 271(1):69–77

    Article  CAS  Google Scholar 

  • Torres RJ, Cesar A, Pastor VA, Pereira CD, Choueri RB, Cortez FS, Morais RD, Abessa DM, do Nascimento MR, Morais CR, Fadini PS (2015) A critical comparison of different approaches to sediment-quality assessments in the Santos estuarine system in Brazil. Arch Environ Contamin Toxicol 68(1):132–147

    Article  CAS  Google Scholar 

  • Umbuzeiro GA, Kummrow F, Roubicek DA, Tominaga MY (2006) Evaluation of the water genotoxicity from Santos Estuary (Brazil) in relation to the sediment contamination and effluent discharges. Environ Int 32(3):359–364

    Article  CAS  Google Scholar 

  • Vuyyuri SB, Ishaq M, Kuppala D, Grover P, Ahuja YR (2006) Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic. Environ Mol Mutag 47(7):562–570

    Article  CAS  Google Scholar 

  • Yamaguchi Y, Madhyastha H, Madhyastha R, Choijookhuu N, Hishikawa Y, Pengjam Y, Nakajima Y, Maruyama M (2016) Arsenic acid inhibits proliferation of skin fibroblasts, and increases cellular senescence through ROS mediated MST1-FOXO signaling pathway. J Toxicol Sci 41(1):105–113

    Article  CAS  Google Scholar 

Download references

Acknowledgments

EAC, DMFS, CDSP, CMC, and DAR are recipients of the CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) fellowships.

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Author notes

  1. Maria Esther Suarez Alpire and Elaine Aparecida de Camargo contributed equally to this work.

Authors and Affiliations

  1. Departamento de Biociências, Universidade Federal de São Paulo, UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos, SP, 11060-001, Brazil

    Maria Esther Suarez Alpire, Caroline Margonato Cardoso & Daniel Araki Ribeiro

  2. Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, Botucatu, SP, Brazil

    Elaine Aparecida de Camargo & Daisy Maria Favero Salvadori

  3. Department of Sea Sciences, Federal University of São Paulo, UNIFESP, Campus Baixada Santista, Santos, SP, Brazil

    Camilo Dias Seabra Pereira

Authors
  1. Maria Esther Suarez Alpire
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  2. Elaine Aparecida de Camargo
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  3. Caroline Margonato Cardoso
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  4. Daisy Maria Favero Salvadori
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  5. Camilo Dias Seabra Pereira
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  6. Daniel Araki Ribeiro
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Correspondence to Daniel Araki Ribeiro.

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This study was approved by Human Ethics Committee from Federal University of São Paulo, UNIFESP, Number # 1.955.311.

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The authors declare that they have no conflict of interest.

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Responsible editor: Philippe Garrigues

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Alpire, M.E.S., de Camargo, E.A., Cardoso, C.M. et al. In vivo and in vitro analysis of cytogenotoxicity in populations living in abnormal conditions from Santos-Sao Vicente estuary. Environ Sci Pollut Res 27, 12039–12046 (2020). https://doi.org/10.1007/s11356-020-07602-0

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